SU1561939A1 - Method of treating hydrobionts - Google Patents

Abstract

The invention relates to agriculture, in particular to methods for processing hydrobionts to produce a feed product. The purpose of the invention is to improve the quality of the target product. In the method of processing hydrobionts, by grinding the raw material, adding acid to the homogenate, followed by exposure and separation of the target product, the acid is added to a pH of 4.5-5.5 at a ratio of sulfur: acid 1: (0.01-0.1), and the exposure Spend 10 to 120 minutes. The method ensures the preservation of the biological value of the target product.

Description

The invention relates to agriculture, in particular to methods for processing hydrobionts to produce a feed product and lipids.

The aim of the invention is to improve the quality of the target product.

The method is carried out as follows.

Raw materials, mainly of lower marketable value (uncut), are ground on a top for subsequent even distribution of acid I in the bulk of the raw material. Acid (any food, mineral or organic) is added in small portions with stirring to pH 4.5-5.5, and the ratio of the crushed raw material: acid solution is 1: 0.01-1: 0.1.

Mixing the acidified mass is carried out for 10-120 minutes at 15-40 ° C until complete separation of the lipid fraction.

The resulting suspension is centrifuged on a three-phase super-decanter to isolate individual fractions differing in density: fat, a dense part and a liquid part (separation factor is not lower than 4000 g). The liquid portion, which is an aqueous enzyme solution and a lipoprotein fraction, is sent for separation. The enzyme solution is concentrated (under vacuum or using ultrasound) to a content of dry matter of 25-30% and sent for final drying (in a spray dryer), obtaining a complex enzyme preparation with a proteolytic activity of 2-4 units / g.

The lipoprotein fraction is a stable emulsion containing 4-6% protein, 40-50% lipids, of which 30-50% are phospholipids. This fraction can be used.

uh

five

for preparing starter feed in fish farming. The dense portion can be used as a feedstuff directly or in dried or canned form. The dense residue, dried under vacuum at 40-80 ° C, is a high-protein (65-75%) feed product with a lipid content of 2-8% of which 30-40% are phospholipids. The proposed method allows to obtain fat in the amount of 60-80% of all lipids of the raw material, and this fraction consists mainly of triglyceri-J5 - (2 months, storage) thawed, from JQ - 5619394

the same fraction (4%), 87 g of fat (7.8%, and 78% of all lipid raw materials) and 707 g (63.1%) of a dense residue. The proteolytic activity of the enzyme preparation is 1 u / g (pH 9), the dried solid residue contains 75% protein and 7% lipids (of which 40% are phospholipids). The content of lipids in the lipoprotein fraction of 39% s protein is 5%, EPA and DHA in the lipid fraction 30%, the acid number of fat is 8S9.

Example 3. 1 kg of undivided Black Sea hamsa frozen

Gov5 fatty acid composition of which is characterized by a high content of lacapentasnova (EPA) and docozagzk-I aenoic (DHA) acids (20-40%).

Example 1. 1 kg of undivided $ (0.015 kg) is centrifuged (factor

3

Black Sea Khamsa is crushed on a top, add 0 „01 kg 6 and hydrochloric acid to rP 5 and mix for 120 minutes at 15 ° C. The resulting suspension (1.01 kg) is centrifuged (separation factor 4,000 g), and the liquid aqueous portion is then separated. Get 234 g of the liquid enzyme fraction (23.1%), 46 g of the lipoprotein fraction (3.6%), 122 g of fat (12.1% and 60% of all lipids in the feed) and 608 g (60.1%) of dense the remainder. The liquid part is spray dried, to obtain an enzyme preparation with a total proteolytic activity of 4 units / g (pH 10.5). The solid residue after drying under vacuum contains 75% protein and 5% lipids (of which 30% are phospholipids). The lipid content in the lipoprotein fraction is 45%, the protein is 4%, the sum of EPA and DHA in lipid fraction is 22%, the acid number of fat is 8.

Thus, the dense residue is enriched in phospholipids, and the lipid fraction

Example 4, The method according to the techniques and operations is identical to the method described in example 3, except that as raw materials use waste from cutting mackerel

fractions (24.1%) s 3.9 g lipoprotein fraction (358%), 114.7 g fat (11.3% and 80E1% of all lipids in the feed)

Chi - polyunsaturated fatty acids, which increases their biological 45 Atlantic (head and inside) value and quality. In addition, 244.6 g of liquid enzyme are obtained.

Two additional products were obtained: a lipoprotein fraction enriched in phospholipids and an enzyme preparation.

Example 2. The method according to the techniques of 50 and 651.8 g (60.8%) of a dense residue. and the operations are identical to the method, the opi-Proteolytic activity of the enzyme in Example 1, except for the preparation of 6.5 U / g (pH 9.2), you use 1 kg of fine Atlantic sardines as raw materials, add 1 N Acetic acid in the amount of 0-10 kg to pH 4.5, stirring is carried out for 10 minutes at 40 ° C. Obtain 281 g of the liquid enzyme fraction (25.1%), 45 g of lipoprotein dense residue contains 65% protein and 10% lipids (of which 40% are phospholipids). The lipid content in the lipoprotein fraction of 36.9% (40% of their amount is phospholipids) of the protein 18% EPA and DHA in the lipid fraction 5%, acid number of fat, 4

crushed on the top, 0S015 kg of 6N hydrochloric acid was added to pH 5.0 and stirred for 60 minutes at 20 ° C. The resulting suspension

0

4000 g), and the liquid aqueous portion is then separated. Get 234 g of the liquid enzyme fraction (23%), 36 g of lipoprotein fraction (3.5%), 122 g of fat (12% and 60% of the total lipids of the raw material) and 623 g (61.5%) of a dense residue. The liquid part is spray dried, to obtain an enzyme preparation with a total proteolytic activity of 4 units / g (pH 10.5). The solid residue after drying under vacuum contains 75% protein and 5% lipids (of which 30% are phospholipids). The lipid content in the lipoprotein fraction is 45% (of which one third is phospholipids), protein is 4%, the sum of EPA and DHA in the lipid fraction is 22%, the acid number of fat is 8 (in the starting material, the acid number of fat is 7).

Example 4, The method according to the techniques and operations is identical to the method described in example 3, except that as raw materials use waste from cutting mackerel

5 Atlantic (heads and entrails) 244.6 g of liquid enzyme are obtained.

fractions (24.1%) s 3.9 g lipoprotein fraction (358%), 114.7 g fat (11.3% and 80E1% of all lipids in the feed)

 Atlantic (heads and entrails) 244.6 g of liquid enzyme are obtained.

 and 651.8 g (60.8%) of a dense residue. The proteolytic activity of the enzyme preparation is 6.5 units / g (pH 9.2), the dried, dense residue contains 65% protein and 10% lipids (of which 40% are phospholipids). The content of lipids in the lipoprotein fraction is 36.9% (40% of their amount is phospholipids), protein 18% EPA and DHA in the lipid fraction 5%, acid number of fat, 4

5136

in the feedstock, the acid number of / -i is 3.2),

EXAMPLE 5. The method is similar to that described in Example 3, only as a raw material, frozen Antarctic krill is used (4 months of storage). 446.6 g of the liquid enzyme fraction (44%), 17.2 g of the lipoprotein fraction are obtained ( 1.7%), 15 g of fat (1.5% by weight of the raw material and 70% of the total lipids of the raw material) and 536.2 g (52.8%) of the dense residue. The proteolytic activity of the enzyme preparation is 30 units / g (pH 9.2) and the dried, dense residue contains 65% protein and 1.5% lipids (of which 40% are phospholipids). The content of lipids in the lipoprotein fraction is 40% (80% of their amount consists of phospholipids), protein 8%, the amount of EPA and DHA in fat 40%, the acid number of fat 3 (in the feedstock the acid number of fat is 2.5).

Compiled by E. Maistrenko Editor A. Shandor Tehred M. Khodanych Corrector S. Shevkun

Order 1017

Circulation 518

VNIIPI of the State Committee on iso-theorems and discoveries at the State Committee on Science and Technology of the USSR 113035, Moscow, Ж-35, Raushsk nab., D. V5

Production and Publishing Combine Patent, Uzhgorod, st. Gagarin, 101

396

Claims (1)

The method allows to obtain 4 fractions in one treatment, each of which can be used as a feedstuff, the liquid part being a complex enzyme preparation, possessing caseinolytic, amino and carboxypeptidase, elastase, and lipolytic activities. The method ensures the safety of biological price-. the target product. Invention Formula The method of processing hydrobionts 5 by grinding the raw material, adding acid to the homogenate, followed by exposure and separation of the target product, characterized in that, in order to improve the quality of the target product, the acid is added to a pH of 4.5-5.5 with a ratio of raw materials: acid 1 : 0.01-0.1, and the exposure is carried out for 10-120 minutes. Subscription