SU1546084A1 - Method of manufacturing artificial lens of the eye - Google Patents

Abstract

The invention relates to medicine, and more specifically to ophthalmology, and can be used in the implantation of an artificial eye lens. The purpose of the invention is to reduce postoperative complications. The artificial lens of the eye contains the optical part and supporting elements, covered with several layers of the proteolytic enzyme. The method of manufacturing the lens involves pressing the optical part and fixing the support elements in the latter. The lens is immersed 3–7 times for 10–30 minutes in a 0.5–10% solution of the proteo- logical enzyme at a temperature of 0–2 ° C, followed by drying the lens at 2–37 ° C. 1 hp f-ly.

Description

The invention relates to medicine, and more specifically to ophthalmology, and can be used in the implantation of an artificial eye lens.

The aim of the invention is to reduce postoperative complications.

The proposed method is carried out as follows.

After pressing the lens and attaching the supporting elements and treating with 10% sodium hydroxide solution for 15-30 minutes (to remove mechanical impurities from the surface), the artificial lens of the eye is washed with distilled water and dried in air. Then the lens is placed in a 0.5-10% solution of the enzyme at a temperature of 0-2 ° C (ice bath) for 10-30 minutes. The lens is removed and dried in air at a temperature of 2-37 ° C for 15-30 minutes (until complete drying). Then the soaking-drying procedure is repeated 2-6 times more. In the control sample of the lens

(one of a series of 10) determine the content and activity of the enzyme. Enzyme-treated lenses are stored in containers at room temperature in opaque shells.

The temperature of the enzyme solution cannot be selected below 0 ° C, otherwise the landing of the enzyme is difficult. At temperatures above 2CC, the activity of planting the enzyme decreases. The concentration of the enzyme solution cannot be less than 0.5%, since otherwise the coating layer may be uneven (intermittent). Over 10% saturation occurs, the activity does not increase. The time of immersion of the lens in the enzyme can not be selected less than 10 minutes, since the landing is insufficient. Over 30 minutes further landing does not increase the activity of the layer. The drying temperature cannot be less than 2CC, because the drying process is too slow. Over 37 ° C denaturation of the enzyme occurs. Drying time is not

cl

-U

ABOUT)

Ltd

Ј

less than 15 minutes can be chosen, since otherwise no complete drying is achieved. Over 30 minutes further drying does not make sense. The number of dives should be at least 3., as the enzyme is rapidly washed out. The artificial lens of the eye is grasped with Binchorst modified tweezers at the equator of the optical zone, the surgeon lifts the cornea with his left hand, and turns the right back alternate for the iris 3 with the back handles. 6–7 corneal scleral sutures are applied to the wound, the anterior chamber is reconstituted with saline to normal depth, a continuous suture is applied to the conjunctiva: Example I.

Temperature pan hole, 0 1 Copetition of thief pact,% o Time of holding in solution, min20 Drying temperature, ° С20 Food unloading time, min 25 Number of dives 5 Accessible activity 52 µg of converted protein per 1 minute per lens. Clinical sedation of the investigated lase came on the fifth day two times faster than the control (tenth day). The maximum response of the eye corresponds to II cieiieiiii (in control 11–111 degrees). Example 2 Solution temperature, ° С 2

0

0

Concentration of the solution,% 10 Soak time in solution, min30 Drying temperature, ° С37 Drying time, min30 Number of dives7 Activity reached 70 µg

transformed protein for 1 min per 1 lens. Clinical sedation of the studied eye occurred on the fifth day 2 times faster than the control one (tenth day). The maximum reaction of the eye corresponds to the I - I1 degree (in the control II - III degree).

Example 3

Solution temperature, ° С О Solution concentration,% 0.5 Soaking time in solution, min10 Drying temperature, ° С2 Time dried, min 15 Number of dives3 Dark protein activity was achieved for 1 min

1.8 mcg transform one lens.

Clinical sedation of the eye after implantation of the lens coated with the enzyme occurred on the sixth day 1.67 times faster than the control head (the tenth day). The maximum reaction of the eye corresponds to the II degree (in the control eye AND III degree).

Claims (2)

1. Artificial lens of the eye, containing the optical part and supporting elements, characterized in that, in order to reduce postoperative complications, the optical part and the supporting elements are covered with several layers of the proteotic enzyme. 2. A method of manufacturing an artificial lens of the eye, which involves pressing the optical part and fixing support elements in the latter, characterized in that, in order to reduce postoperative complications, the artificial lens is dipped 3–7 times for 10–30 minutes in 0.5–10% - a proteolytic enzyme solution was temperature 0-2 ^C, followed drying the lens with 2- 37 ° C.