SU149185A1 - Method for studying intestinal activity - Google Patents

Description

Methods for studying intestinal activity by imposing a fistula from living tissue with the removal of a loop of the intestine into a skin flap are known.

It is proposed to impose several fistulas depending on the intestinal section under study. This will allow to study the secretion and motor activity of the intestines in different areas, the rate of mixing of intestinal contents, enter stimuli into the intestinal lumen, as well as measure the temperature in the intestines.

FIG. 1 shows a diagram of the incisions on the skin of an experimental animal; in fig. 2 a, b - the formation of fistula; in fig. 3 shows the layout of the fistula and intestinal loop.

The formation of fistulas and intestinal loops is carried out in the following manner.

A skin incision 1, 3-5 cm long, is made on the skin of the experimental animal from the urinary process in the midline (Fig. 1). In addition, two lateral incisions are made, each 7-10 seconds long: one cut 2 is the outer nipple and the other 3 is medial to them. the distance between them is 6-7 cm. In addition, the median and nearby lateral incisions / and 5 are connected by an additional transverse incision 4. The skin between the lateral incisions is removed from the adjacent tissues.

The skin flap formed in this way is retracted into CTOpOj, the abdominal cavity is opened, and, depending on the goal, a certain loop of the intestine is removed. Consider a specific case of fistula formation from the duodenum.

The part of the duodenum, located distal from the site of attachment of the pancreas to it for 1-2 cm, is mobilized. Along the anterior margin of the mobilized area, the gut intersects. A fistula is formed from its peripheral end (Fig. 2 a, b, GV Orlov method). For this 2.5-4 cm peripheral

No. 149185- 2 of the segment of the intestine from the side opposite to the breeching, is sutured by a continuous two-stitched seam 5 and three rows of single seams, o. The base diameter of the cone 7 formed in this way should be 2-2.5 times smaller than the diameter of the intestine 8. The cone formed from the intestine 8 (7–2 cm from the base of it) invaginates into the intestinal lumen (Figs. 2 b and 3).

The restoration of the intestinal permeability is achieved by ligation of the central segment 9 of the "cut" intestine into the peripheral side, 3-5 cm from the fistula (Fig. 4a).

Then, a loop of 12 duodenal or small intestine (10-12 cm long) is selected to bring it into the skin flap (Fig. 3). An intestinal fistula is fixed by several sutures 11 to the peritoneum. An indentation of 2-4 cm from the fistula is removed outside the abdominal cavity of the intestinal loop W.

After suturing, an aneurosis forms a skin flap. To do this, the sutures are first applied at the cross-section 4 (Fig. 1), and then on the skin flap, with the intestinal loop 10 inserted into it. The final stage is suturing, tightening the skin of the anterior abdominal wall. For this, in the incision area 2 (Fig. 1), the muscular layer is also captured in order to attract the skin from the opposite side. Since the lateral incisions 2 and 3 were made at different distances from the midline, the sutures on the skin graft on the anterior abdominal wall do not match, which contributes to a better formation of the postoperative scar.

If necessary, a fistula can be formed from the central end of a cut intestine or several fistulas, depending on the radiated part of the intestine, with the removal of several intestinal loops into skin grafts.

A rubber tube with a diameter of 0.3–0.8 cm is easily inserted into the fistula to introduce irritants into the intestine, to take intestinal contents, etc. The clamping of the intestinal loop 10 with a rubber cuff makes it possible to make a proper study of the intestinal motor activity, the speed of moving its contents, etc.

Subject invention

A method for studying intestinal activity by applying a fistula from living tissues with removing a loop of intestine into a skin flap, characterized in that, in order to study the secretion and motility of the intestine in different areas, the speed of movement of the intestinal contents, the introduction of irritants into the intestinal lumen, measure the temperature in it , impose several fistulas depending on the area of the intestine.

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