SU1448235A1 - Method of diagnosis of ureaplasmosis - Google Patents

Method of diagnosis of ureaplasmosis Download PDF

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Publication number
SU1448235A1
SU1448235A1 SU853923521A SU3923521A SU1448235A1 SU 1448235 A1 SU1448235 A1 SU 1448235A1 SU 853923521 A SU853923521 A SU 853923521A SU 3923521 A SU3923521 A SU 3923521A SU 1448235 A1 SU1448235 A1 SU 1448235A1
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SU
USSR - Soviet Union
Prior art keywords
ureaplasmosis
olivomycin
minutes
coloring
accuracy
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Application number
SU853923521A
Other languages
Russian (ru)
Inventor
Иван Иванович Мавров
Александр Евгеньевич Карпенко
Галина Романовна Михайлова
Original Assignee
Харьковский научно-исследовательский институт дерматологии и венерологии
Научно-исследовательский институт вирусологии им.Д.И.Ивановского
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Application filed by Харьковский научно-исследовательский институт дерматологии и венерологии, Научно-исследовательский институт вирусологии им.Д.И.Ивановского filed Critical Харьковский научно-исследовательский институт дерматологии и венерологии
Priority to SU853923521A priority Critical patent/SU1448235A1/en
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Publication of SU1448235A1 publication Critical patent/SU1448235A1/en

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Abstract

Изобретение относитс  к медицн- не. Дл  повьшени  точности диагностики каплю спермы фиксируют, окрашивают оливомицином в течение 50- 60 мин и микроскопируют. Уреаплазмоз определ ют по свечению ДОК уреаппазм в сперматозоидах.The invention relates to medicaments. In order to improve the accuracy of diagnosis, a drop of sperm is fixed, stained with olivomycin for 50-60 minutes and microscopically examined. Ureaplasmosis is determined by the luminescence of DOC ureappasm in sperm.

Description

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Изобретение относитс  к медицине, а именно к микробиологии, и может быть использовано дл  вы влени  микроплазменного поражени  спермы.The invention relates to medicine, namely microbiology, and can be used to detect microplasma lesions of sperm.

Цель изобретени  - повышение точности способа.The purpose of the invention is to improve the accuracy of the method.

Способ осуществл ют следующим образом .The method is carried out as follows.

Каплю спермы нанос т на предметное стекло и довод т до размеров покровного стекла. После высушивани  на воздухе мазок фиксируют в охлажкаплю забуференного глицерина и накрывают покровным стеклом, обвод т парафином и микроскопируют в падаю- щем свете люминисцентного микроскопа . Уреаплазмоз определ ют по  рко- зеленому свечению ДНК уреаплазм в сперматозоидах.A drop of sperm is applied to a glass slide and adjusted to the size of a cover glass. After air drying, the smear is fixed in a cooling unit of buffered glycerol and covered with a cover glass, coated with paraffin and microscoped in the falling light of a fluorescent microscope. Ureaplasmosis is determined by the bright green luminescence of ureaplasma spermatozoa.

Claims (1)

JQ Формула изобретениJQ claims Способ диагностики уреаплазмоза путем приготовлени  мазков из семен ной жидкости, их фиксации и окраскиA method for diagnosing ureaplasmosis by preparing smears from seminal fluid, fixing and coloring them денном до +4°С этиловом (70 ) спирте 15 отличающийс  тем, что,Date to + 4 ° C ethyl (70) alcohol 15 characterized in that каплю забуференного глицерина и накрывают покровным стеклом, обвод т парафином и микроскопируют в падаю- щем свете люминисцентного микроскопа . Уреаплазмоз определ ют по  рко- зеленому свечению ДНК уреаплазм в сперматозоидах.A drop of buffered glycerol is covered with a cover glass, coated with paraffin and microscoped in the falling light of a fluorescent microscope. Ureaplasmosis is determined by the bright green luminescence of ureaplasma spermatozoa. Формула изобретени Invention Formula Способ диагностики уреаплазмоза путем приготовлени  мазков из семенной жидкости, их фиксации и окраски,A method for diagnosing ureaplasmosis by preparing smears from seminal fluid, their fixation and staining, в течение 1 ч. Затем на препарат нанос т рабочий ра.створ оливомицина и помещают его в темную камеру на 60 мин.for 1 hour. Then a working solution of olivomycin is applied to the preparation and placed in a dark chamber for 60 minutes. После быстрой промывки в дистил- 20 редел ют по свечению ДНК уреаплазм в лированной воде на препарат нанос тсперматозоидах.After rapid washing in distilled 20, the luminescence of ureaplasma DNA in molded water is determined on the preparation by means of spermatozoa. с целью повьшани  точности способа, окраску провод т в течение 50-60 мин при этом в качестве красител  исполь зуют оливомицин, а уреаплаэмоз опс целью повьшани  точности способа, окраску провод т в течение 50-60 мин, при этом в качестве красител  используют оливомицин, а уреаплаэмоз опin order to improve the accuracy of the method, the coloring is carried out for 50-60 minutes, while olivomycin is used as a dye, and ureaplaemosis is used to increase the accuracy of the method, the coloring is carried out for 50-60 minutes, while olivomycin is used as a dye, and ureaplaemosis op
SU853923521A 1985-05-30 1985-05-30 Method of diagnosis of ureaplasmosis SU1448235A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU853923521A SU1448235A1 (en) 1985-05-30 1985-05-30 Method of diagnosis of ureaplasmosis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU853923521A SU1448235A1 (en) 1985-05-30 1985-05-30 Method of diagnosis of ureaplasmosis

Publications (1)

Publication Number Publication Date
SU1448235A1 true SU1448235A1 (en) 1988-12-30

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Family Applications (1)

Application Number Title Priority Date Filing Date
SU853923521A SU1448235A1 (en) 1985-05-30 1985-05-30 Method of diagnosis of ureaplasmosis

Country Status (1)

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Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Делекторский В.В. и др. Уреа- плаэма. Лабораторна диагностика, Тбилиси, 1983, 13. *

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