SU1409886A1 - Method of preparing biological material for histological investigation - Google Patents
Method of preparing biological material for histological investigation Download PDFInfo
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- SU1409886A1 SU1409886A1 SU864048703A SU4048703A SU1409886A1 SU 1409886 A1 SU1409886 A1 SU 1409886A1 SU 864048703 A SU864048703 A SU 864048703A SU 4048703 A SU4048703 A SU 4048703A SU 1409886 A1 SU1409886 A1 SU 1409886A1
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Abstract
Изобретение относитс к гистологии . Цель изобретени - повышение качества срезов. Биологический материал фиксируют в формалине, обезвоживают в возрастакхцих концентраци х этилового спирта с последующим пропитыванием в среде, содержащей кси- лол, кедровый бальзам и касторовое масло. На основе указанной смеси готов т смесь А и смесь Б. Смесь А содержит 80% химически чистого ацетона и 20% исходной смеси. Смесь Б состоит из 20% ацетона и 80% исходной смеси. Обрабатывают ткани в этиловом спирте в течение 1-5 ч. Перенос т материал в 2 порции смеси Айв 2 пор ции смеси Б. Затем осуществл ют пропитку в 6 порци х парафина 56- 60°С по 25-30 мин в каждой. Способ позвол ет сократить врем , необходимое дл получени парафиновых блоков. (ЛThis invention relates to histology. The purpose of the invention is to improve the quality of cuts. The biological material is fixed in formalin, dehydrated in increasing concentrations of ethyl alcohol, followed by impregnation in a medium containing xylene, cedar balsam and castor oil. Based on this mixture, a mixture of A and B is prepared. Blend A contains 80% of chemically pure acetone and 20% of the initial mixture. Mixture B consists of 20% acetone and 80% of the initial mixture. Treat the tissues in ethyl alcohol for 1-5 hours. Transfer the material in 2 portions of the mixture Aive 2 portions of the mixture B. Then infiltrate in 6 portions of paraffin 56-60 ° C for 25-30 minutes each. The method allows to reduce the time required to obtain paraffin blocks. (L
Description
Изобретение относитс к медицине и биологии.The invention relates to medicine and biology.
Целью изобретени вл етс повышение качества срезов за счет исклю- чени деформирующего воздействи ксилола.The aim of the invention is to improve the quality of the cuts by eliminating the deforming effect of xylene.
Способ осуществл ют следующим образом .The method is carried out as follows.
Биологический материал фиксируют в i acTBOpe формалина $ далее обезвоживают в возрастающих концентраци х этилового спирта с последующим пропи- тьгоанием в среде, содержащей ксилол, кедровый или пихтовый бальзам и кас- торовое масло в соотношении 90-110, 9-11, 14-16 соответственно. На основе указанной смеси готов т смеси А и Б . Смесь А содержит 80% химически чистого ацетона и 20% исходной смеси Смесь Б состоит из 20% ацетона и 80% исходной смеси. Обрабатывают кусочки тканей в этиловых спиртах 96 , использу две порции последнего, общей продолжительностью 1-5 ч. Перенос т материал в 2 порции смеси А (по 1,5- 3 ч в каждой) и в 2 порции смеси Б jCno 1,5-2 ч в каждой). Затем осуществл ют пропитку в 6 порци х на при 56-60 С по 25-30 мин в каждой The biological material is fixed in i acTBOpe formalin $, then dehydrated in increasing concentrations of ethyl alcohol, followed by feeding in a medium containing xylene, cedar or fir balsam and caster oil in a ratio of 90-110, 9-11, 14-16, respectively . Based on this mixture, mixtures A and B are prepared. Mixture A contains 80% chemically pure acetone and 20% of the initial mixture. Mixture B consists of 20% acetone and 80% of the initial mixture. Treat the tissue pieces in ethyl alcohols 96, using two portions of the latter, with a total duration of 1-5 hours. Transfer the material in 2 portions of mixture A (1.5 to 3 hours each) and in 2 portions of mixture B jCno 1.5- 2 hours each). Then impregnation is carried out in 6 portions at 56-60 ° C for 25-30 minutes in each
П р и м.е р.,Кусочки биопсийного, операционного и аутопсийного материала (по площади от 2x2 мм до половины предметного стекла) фиксируют вPr and m.e. p., Pieces of biopsy, surgical and autopsy material (in area from 2x2 mm to half of a glass slide) are fixed in
10%-ном растворе формалина, промывают10% formalin solution, washed
д 5 0 5 0d 5 0 5 0
5five
в проточной воде и обсушивают, после чего последов ательно обрабатывают в 96 -ном этиловом спирте в 2-х порци х по 30 мин в каждой, в смеси А - в 2-х порци х по 3 ч в каждой, в смеси Б - в 2-х порци х по 2 ч в каждой , в парафине - при 56-60 С в 6 порци х по 30 мин в каждой, после чего заливают кусочки в парафин.in running water and dried, after which they are sequentially treated in 96% ethanol in 2 portions for 30 minutes each, in mixture A - in 2 portions for 3 hours each, in mixture B - in 2 portions of 2 hours each, in paraffin - at 56-60 ° C in 6 portions of 30 minutes each, then pour the pieces in paraffin.
Среды, обработанные предложенным способом, не содержат деформации ткани , не пересушены, хорошо восприни- . мают красители.Environments treated by the proposed method do not contain tissue deformation, not overdried, well perceived. dye dyes.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU864048703A SU1409886A1 (en) | 1986-01-23 | 1986-01-23 | Method of preparing biological material for histological investigation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU864048703A SU1409886A1 (en) | 1986-01-23 | 1986-01-23 | Method of preparing biological material for histological investigation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1409886A1 true SU1409886A1 (en) | 1988-07-15 |
Family
ID=21230732
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU864048703A SU1409886A1 (en) | 1986-01-23 | 1986-01-23 | Method of preparing biological material for histological investigation |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1409886A1 (en) |
-
1986
- 1986-01-23 SU SU864048703A patent/SU1409886A1/en active
Non-Patent Citations (1)
| Title |
|---|
| Лилли Р. Патогистологическа техника и практическа гистохими , М., 1969. * |
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