SU92222A1 - The method of manufacture of histological preparations - Google Patents
The method of manufacture of histological preparationsInfo
- Publication number
- SU92222A1 SU92222A1 SU434341A SU434341A SU92222A1 SU 92222 A1 SU92222 A1 SU 92222A1 SU 434341 A SU434341 A SU 434341A SU 434341 A SU434341 A SU 434341A SU 92222 A1 SU92222 A1 SU 92222A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- manufacture
- histological preparations
- preparations
- stained
- polyvinyl alcohol
- Prior art date
Links
- 238000002360 preparation method Methods 0.000 title description 7
- 238000004519 manufacturing process Methods 0.000 title description 4
- 239000004372 Polyvinyl alcohol Substances 0.000 description 6
- 229920002451 polyvinyl alcohol Polymers 0.000 description 6
- 239000006059 cover glass Substances 0.000 description 3
- 229920002037 poly(vinyl butyral) polymer Polymers 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- CTQNGGLPUBDAKN-UHFFFAOYSA-N o-xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- 239000008096 xylene Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drugs Drugs 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229920000591 gum Polymers 0.000 description 1
- 210000000056 organs Anatomy 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 210000001519 tissues Anatomy 0.000 description 1
Description
Предметом изобретени вл етс способ приготовлени проб живых тканей дл микроскопического исследовани , т. е. гистологических препаратов без применени покровных стекол.The subject of the invention is a method of sampling living tissue for microscopic examination, i.e., histological preparations without the use of a cover glass.
Дл заключени гистологических срезов, окрашенных обычным способом, в прозрачные среды примен ют гумми-сироп, глицеринжелатину , глицерин-левулезу и т. д., причем во всех случа х требуетс применение покровных стекол. Однако эти вещества имеют недостатки: в глицерин-желатине препараты быстро выцветают, а в гуммисиропе , глицерин-левулезе могут развиватьс процессы брожени , гниени и т. д.Gum syrup, glyceroline-gelatin, glycerol-leukemia, etc., are used in transparent media to conclude histological sections stained in the usual way, and in all cases the use of a cover glass is required. However, these substances have drawbacks: in glycerin-gelatin, preparations quickly fade, and in gumsyrope, glycerin-levulosis, fermentation, rotting, etc. can occur.
Предлагаемый способ устран ет указанные недостатки. При нриготовлении гистологических препаратов, окрашенных на жир, примен ют раствор поливинилового спирта, а в остальных случа х - поливинилбутираль , причем в обоих случа х не требуетс применени покровных стекол. Кроме того, предлагаемым способом удаетс изготовл ть большие гистотопографические препараты, т. е. срезы через весь орган, например какую-либо из стенок сердца.The proposed method eliminates the indicated disadvantages. In the preparation of histological preparations stained with fat, a solution of polyvinyl alcohol is used, and in other cases polyvinyl butyral is used, and in both cases no cover glasses are required. In addition, the proposed method makes it possible to manufacture large histotopographic preparations, i.e. sections through the entire organ, for example, one of the walls of the heart.
Способ заключаетс в следующем- Предварительно приготовл ют 10-12%-ный раствор поливинилового спирта, дл чего порощок поливинилового спирта промывают в 96° спирте, затем в эфире и подсушивают . После этого поливиниловый спирт раствор ют в дистиллированной воде. Далее, срез, окрашенный на жир, помещают на предметное стекло и нанос т на него несколько капель приготовленного раствора поливииилового спирта, который через 6-8 час. высыхает, образу прочную гладкую пленку, в которой заключен срез. Дл заключени срезов, окрашенных другими способами, приготовл ют 12-15%-ный раствор поливинилбутирал в карболксилоле или спиртксилоле . В дальнейшем при изготовлении препарата поступают так же, как и при заключении срезов, окрашенных на жир.The method consists in the following: A 10–12% solution of polyvinyl alcohol is preliminarily prepared, for which the powder of polyvinyl alcohol is washed in 96 ° alcohol, then in ether and dried. After that, polyvinyl alcohol is dissolved in distilled water. Next, a cut stained for fat is placed on a glass slide and a few drops of a prepared solution of polyvinyl alcohol are applied to it, which after 6-8 hours. dries to form a durable smooth film, which encloses the cut. For the conclusion of sections stained by other methods, a 12-15% solution of polyvinyl butyral in carbol xylene or alcohol xylene is prepared. In the future, in the manufacture of the drug come the same way as in the conclusion of sections stained for fat.
Л 92222- 2 -L 92222-2 -
П р е д м с т и 3 о б р е т е и и PREDIMT AND 3 ABOUT THEM and and
Способ изготовлени гистологических препаратов с заключением срезов в прозрачные застывающие массы, отличающийс тем, что окращенные и обработанные обычным путем срезы заливают поливинилбутиралем, а при окраске на жир - поливиниловым спиртом.A method of making histological preparations with the conclusion of sections in transparent solidifying masses, characterized in that the sections which have been cut and processed in the usual way are filled with polyvinyl butyral, and when stained for fat, with polyvinyl alcohol.
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU92222A1 true SU92222A1 (en) | 1951-10-31 |
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