SU1388429A1 - Method of controlling process of preparation of culture medium salt solution - Google Patents

Description

i (2I) 4136917 / 28-13 1 (22) 10.17.86 (46) 04.15.88.BYL. Number 14

(71) Grozny Scientific Production Association Promavtomatika

(72) N.S.Yaybin, V.P.Pimenny, A.A. Kukharenko and O.P. Serchenko (53) 653.G. (088.8)

(56) Intensification of technological processes for the production of PVC of purified liquid paraffins on production lines with a capacity of 35 and 60 thousand tons per year. - Report of the All-Russian Research Institute of Synthesis of Proteins. vol. IV, Automation of production processes, state registration number 76063995, inv. No. 667731. M .: VNTI-Center, 1978, UDC 582.228: 663.15: 661, 716.1.

(54) METHOD OF MANAGING THE PROCESS OF PREPARATION OF SALT NUTRIENT MEDIUM SOLUTION

(57) The invention relates to methods for controlling the process of preparing nutrient media and is aimed at improving the quality of the nutrient media. The required salt consumption in the saline solution of the nutrient medium is set and a saturated solution of this salt is prepared. The temperature of the solution used is measured, and the required water consumption is calculated in a computing device at a given salt consumption and the temperature of the saturated solution. The current value of the water flow in the mixer is measured and, depending on the mismatch between the current and calculated water flow, the flow rate is affected. I il.

with "

(L

one

The invention relates to methods for controlling the process of preparing nutrient media and can be used in microbiological, pharmaceutical and medical industry.

The purpose of the invention is to improve the quality of the nutrient medium by stabilizing the flow of salt supplied with a saline solution.,

The drawing shows a diagram of the device that implements the proposed control method.

The control unit includes a water flow sensor 1 connected to a controller 2 acting on a valve 3 installed on the water supply line to the mixer 4, a temperature sensor 5 whose output signal goes to a computing device 6 containing a multiplication unit 7 that is connected to the summation unit 8, and the latter is connected to the division unit 9, the second input of which is connected to the setting device 10, and the output is connected to the controller 2 ..

The device works as follows.

Sufficient dry salt is poured into the mixer 4 in order to prepare a saturated solution for a long period of time (shift, day or more). The concentration of the saturated solution is determined only by its temperature. A vessel with overflow is used as a mixer 4 and the flow rate of the solution at the outlet of the mixer is determined by the expression

de q

R

 K-Q. and -j

(1) 40

TG 1 ..

volumetric costs, respectively, of the solution discharged from the mixer, and the water supplied to the mixer, l / g;

constant coefficient.

45

The amount of salt dissolved per unit time can be expressed in terms of the flow rate and concentration of the solution withdrawn from the mixer.

GC Q,

with

R)

(2)

where Ср is the concentration of the solution withdrawn from the mixer,

Substituting Qp from (1) (2) gives:

Gc Kcp

(3)

If a mixed solution is obtained in the mixer, then. its concentration Cp is determined by the temperature in the mixer (T).

When the temperature T varies within 5-10 ° C, as happens in production, the dependence of the concentration of the saturated solution on temperature is quite accurately described by a linear equation

a + w

(four)

where a, b are constant coefficients

proportions, which are determined by the least squares method from reference data on the concentration and temperature of the saturated salt solution. Substituted the value of Cf: from form (4) and

Q.

(3), receive: Gt.

C + dT

(five)

30 5

40

45

50

55

where with to, and; d to-in

When the temperature in the mixer rises, the output signal of the temperature sensor 5 increases and enters the multiplication unit 7 of the computing device 6, where the temperature T is multiplied by. the constant d, 4To leads to an increase in the product dT, and hence the output signal of block 7. By increasing the product dT supplied from the output of multiplication unit 7 to the input of summation unit 8, performing summation of the product dT and constant value C. The output signal of block 8 corresponding to the sum of C + dT increases and enters the input of block 9, where the value of G coming from setpoint 10 is divided by C + dT in accordance with formula (5) that. leads to a decrease in the output signal of the dividing unit 9, which is supplied as a reference to the water flow regulator 2.

The reduction of the task to the controller 2 leads to the formation of an effect on the valve 3, which reduces the flow rate of 5

ten

15

31388429

As a result of reducing the flow of water into the mixer, the preset value of the salt fed to the nutrient medium is restored using the control system.

When the temperature T in the mixer decreases, the output signal of the temperature sensor 5 decreases, which leads to an increase in the value of p5 calculated by blocks 7–9 of the computing device 6 using formula (5). Consequently, the output-one signal of dividing unit 9 increases, which leads to an increase in the setting of the flow controller 2, which forms a control action on the regulating valve 3, which increases the flow of water to the mixer.

Since the concentration of the saturated solution decreases with decreasing temperature 5T4, the amount of salt supplied to the nutrient medium decreases while maintaining the flow rate constant. As a result of the increase in the flow of water to the mixer, the desired amount of salt fed to the nutrient medium is restored using the control system.

If necessary, increase

20

25

thirty

supplying salt G to the nutrient medium using sensor O increases the reference signal supplied to block 9 of the computing device 6, where calculated by the formula

О .L- 4 C + dT

Yes, water 2 is increased, so that bringing the controller to the flow rate

calculated by the formula Q "

If it is necessary to reduce the salt supply G (. To the nutrient medium by the sensor 10, the reference signal is reduced, which is supplied to the dividing unit 9 of the computing device 6, where

- CH-df

setting the water flow controller 2 is reduced, resulting in a decrease in the flow of water supplied to the mixer.

The implementation of the proposed control method allows one to maintain the concentration of salts in the nutrient medium strictly at a given level, which improves the quality of the nutrient medium and, consequently, the yield of the target product and the fermentation rate -.

Claims (1)

Invention Formula The method of controlling the preparation of a salt solution of a nutrient medium, which involves obtaining a salt solution, selecting it from the mixer, and regulating the flow of water to the mixer, characterized in that, to increase the quality of the nutrient medium, the required flow rate of the salt solution is measured. in the mixer and calculate the required water flow, measure the current value of water flow in the mixer, and the regulation of the flow of water into the mixer is carried out by Dit is to open valve 3 on the line-40 mismatch between the current and Giving water to the mixer calculated water flow rates.