SU1388419A1 - Laboratory installation for cultivating microorganisms - Google Patents

Abstract

The invention relates to laboratory installations for injecting microorganisms. The goal is to reduce the loss of nutrient components and increase the accuracy of dosing. The installation contains a fermenter 1, a system for metering components of the nutrient medium, including dispensers 2 and 3 and outlet pipes 4 and 5 connected to them, a cone-shaped chamber

Description

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6 with a nozzle 7 installed inside it, a pipeline 9 with a valve 10, valves 11,12,13,14,15,16 and 17 connected to the control unit 18, pressure sensors 19,20, gas cylinder 21, receiver 22 , collector 23, agitator 24, aerator 25 with valve 26, defoamer 27, condenser with throttle 29 and filter 30. In the proposed installation, the entire dosed nutrient medium is supplied to the culture fluid with preliminary mixing of nutrients and their aeration about 1 sludge.

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The invention relates to laboratory plants for growing microorganisms.

The aim of the invention is to reduce the loss of nutrient components and to improve the accuracy of determining the effect of these components on the growth of microorganisms.

The drawing shows a plant for cultivating microorganisms, a longitudinal section.

The installation contains a fermenter 1 and a system for metering components of the nutrient medium, including dispensers 2 and 3 and outlet pipes 4 and 5 connected to them for individual components of the nutrient medium, a hermetic cone-shaped chamber 6 for pre-mixing the components of the nutrient medium provided with a nozzle 7 located inside it for supplying gas. a, while the outlet nozzles 4 and 5 are placed inside the chamber 6 in such a way that their outlet openings are located under the nozzle 7 and wipe each other to form an ejector 8, and The chamber 7 is connected to the body of the fermenter 1 by means of a pipeline 9 with a valve 10. In addition, the installation includes valves 11-17, sensors and pressure sensors 19 and 20 connected to the control unit 18, a gas cylinder 21 and a reservoir 22 connected to it 23, mounted in the lower part of chamber 6, agitator 24 and aerator 25 with a check valve 26, placed in the lower part of the fermenter 1, distiller 27, condenser 28 with throttle 29 and filter 30 placed in the upper part of the fermenter 1 o

The installation works as follows.

On the control signal of the unit 18, the valve 10 is closed and the valve 17 is closed.

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opens up. The valve 13 is opened and the gas from the cylinder 21 through the receiver 22 is fed to the nozzle 7, which forms a gas jet. Dispensers 2 and 3 feed the nutrient components through check valves 11 and 12 into the pipes 4 and 5 o. From the pipes 4 and 5, the withdrawn liquid enters the chamber 6c. Under the action of the gas jet formed by the nozzle 7, a drop of liquid nutrients is completely removed. the outlet openings of pipes 4 and 5. A gas jet disperses and mixes drops of liquid nutrients. Moving in a stream of gas, the liquid droplets are saturated with gas. Upon entering the conical wall of chamber 6, the droplets become larger and flow to the outlet of chamber 6 in the region of the location of the collector 23. When the chamber 6 is filled with a mixture of liquid nutrients, the air from chamber 6 is forced out through the valve 17 to the atmosphere. After dispensers 2 and 3 supply the required dose of nutrients to chamber 6, block 18 turns off dispensers 2 and 3, closes valves 13 and 17 and opens valve 14 "Gas through valve 14 enters collector 23 and through holes in the wall chambers 6 bubbled into the mixture of nutrients, additionally airing it and mixing holes in the wall of chamber 6 are made with such a diameter that the liquid mixture does not enter the collector 23. Pressure in chamber 6 begins to increase. The overpressure in chamber 6 closes the check valves 11 and 12, which eliminates the effect of overpressure on the dispensers 2 and 3. When the pressure in chamber 6, determined by the signal from sensor 19, reaches, the value is greater than the pressure in

the fermenter, determined by the signal from sensor 20, block 18 opens valve 13 and valve 10. Under the action of overpressure in chamber 6 and a gas jet from nozzle 7, the dose of the mixture of nutrients through pipe 9 is completely displaced into the culture fluid T & into the zone of action of the mixing device 24. The gas jet coming out of the pipe 9 together with the dose of the mixture of nutrients mixes the culture liquid and aerates it with the gas flow coming from the aerator 25 o

Entering the fermenter 1 with a pressure lower than the pressure in chamber 6, at which the mixture of liquid nutrients saturates with gas, the mixture of liquid components boils and gas, which provides additional aeration and mixing of the culture liquid with the mixture of nutrients. In this mode of operation of the plant, the dosing of liquid nutrients is performed by dispensers operating at normal pressure, and the dose of the mixture of nutrients can be supplied to the fermenter operating at elevated pressure,

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For the continuous supply of a mixture of nutrients to the fermenter 1, block 18 closes valve 17 and opens valve 10 and 13. Dispersed gas-liquid mixture through pipes 9 enters the fermenter 1 ". Necessary pressure drop of gas between chamber 6 and fermenter 1 via sensor signals 19 and 20 is provided by a block 18, which regulates the flow area of the throttles 29 and switches the valves 13 and 17.

The gas flow to the aeration is fed through the valve 15 and the reverse valve 26 to the aerator 25, the exhaust gas through the condenser 28, adjustable

choke 29 and filter 30 goes to atmosphere

Drainage of the culture fluid to maintain the desired level is provided through the valve 16, the foam is quenched by the defoamer 27,

In this laboratory setup, the loss of nutrient mixture components is reduced by supplying the entire dosed fluid to the culture medium with a gas stream with preliminary mixing of nutrients and their aeration.

The supply of lossless nutrient components to the culture-free medium without loss increases the accuracy of determining the influence of these components on the growth of microorganisms, especially when dosing.

Claims (1)

Invention Formula A laboratory plant for the cultivation of microorganisms, containing a fermenter and a system for metering nutrient medium components, including metering units and esshechny pipes connected to them for individual components of the nutrient medium, characterized in that, in order to reduce losses of nutrient medium components and the possibility of increasing the accuracy of determining the effects of these components on the growth of microorganisms, the installation contains a sealed cone-shaped chamber for pre-mixing the components of the nutrient medium dyes equipped with a gas supply nozzle located inside it, while the outlet nozzles for the individual components of the nutrient medium are placed inside this chamber so that their outlet openings are located under the nozzle opposite each other to form an ejector, the chamber being connected to the body of the fermenter through a pipeline with valve.