CN109266515A - A kind of closed aerosol generating device - Google Patents

A kind of closed aerosol generating device Download PDF

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CN109266515A
CN109266515A CN201811124337.8A CN201811124337A CN109266515A CN 109266515 A CN109266515 A CN 109266515A CN 201811124337 A CN201811124337 A CN 201811124337A CN 109266515 A CN109266515 A CN 109266515A
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cylinder
gas
aeration
generating device
aerosol generating
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CN109266515B (en
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韩云平
杨唐
刘俊新
李琳
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Research Center for Eco Environmental Sciences of CAS
University of Chinese Academy of Sciences
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University of Chinese Academy of Sciences
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    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J13/00Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
    • B01J13/0095Preparation of aerosols
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers

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Abstract

The present invention proposes a kind of closed aerosol generating device, belongs to atmosphere environment supervision technical field.The diffusion mechanism that generating device includes generation mechanism, is connected above generation mechanism and with generation mechanism;Generation mechanism includes waste liquid cylinder and the aeration cylinder positioned at waste liquid cylinder top, the overflow launder of annular is formed between the side wall and gear liquid wall of aeration cylinder, the bottom of overflow launder is equipped with the overflow port for flowing to waste liquid cylinder, the inner bottom of aeration cylinder is equipped with bubble dish, water inlet pipe is equipped in aeration cylinder and for the air inlet pipe one to bubble dish conveying gas;Diffusion mechanism includes gas mixer chamber, boring aeration circle and multiple gas expanded cylinders, and the side wall of gas mixer chamber is connected with gear liquid wall, and the gas expanded cylinder on top is equipped with reserved thief hatch and detection mouth.The present invention can stablize, efficiently complete laboratory scale the microbial aerosol based on sewage (mud) generation.

Description

A kind of closed aerosol generating device
Technical field
The present invention relates to atmosphere environment supervision technical fields, and in particular to a kind of closed aerosol generating device.
Background technique
Comprising a variety of materialization ingredients, microorganism and its metabolic components etc. in sewage (mud), in driving effects such as aeration, stirrings Under, it is spilt into air in the form of microbial aerosol.Virulence factor wherein included, such as pathogenic bacteria, virus, toxin Deng to sewage treatment plant worker and nearby residents, there are health threats.Therefore, sufficiently verify microbial aerosol component and Existing forms, generting machanism and loss feature, so that the control for sewage treatment plant's microbial aerosol provides reference, while right The hygiene and health of sewage treatment plant worker and its nearby residents also have great importance.
Currently, the research about sewage treatment plant's microbial aerosol loss feature focuses primarily upon sewage treatment plant scene Investigation, but the limitation of needs for stable operation under specific operating condition and sewage plant processing unit construction, generting machanism and The controllability of loss characteristic aspect research is not strong.
In addition, traditional micro-organism aerosol generating apparatus based on bubbles burst, exist occur liquid be repeated shearing with And disturbance of the enclosure space interior air-flow to not aerosolized generation liquid, so that the aerosol particle physical property matter finally measured is by shadow It rings.
Therefore, in order to avoid the field research of traditional wastewater treatment plant and traditional microbiological aerosol generating device the drawbacks of, A kind of stabilization, efficient Wastewater Microbial Aerosols generating device are developed, microbial aerosol component, existing forms, life are carried out Research at mechanism and loss characteristic aspect has important practical value and realistic meaning.
Summary of the invention
For above-mentioned current research and problems of the prior art, the present invention provides a kind of stabilizations, efficient base In the closed aerosol generating device of the double-deck blast aeration, can overcome by can existing for sewage treatment plant's field research Control property is not strong, the problem of being easily affected by other factors;It can avoid that liquid repetition shearing occurs to greatest extent again and reduce closing Disturbance of the space interior air-flow to not aerosolized generation liquid.
A kind of closed aerosol generating device, including generation mechanism, be located at generation mechanism above and with generation mechanism phase The diffusion mechanism of connection;
Generation mechanism includes closing the waste liquid cylinder of setting, the aeration cylinder of top opening setting and on the outside of aeration cylinder Liquid wall is kept off, aeration cylinder and gear liquid wall are respectively positioned on the top of waste liquid cylinder, form overflowing for annular between the side wall and gear liquid wall of aeration cylinder Chute, the bottom of overflow launder are equipped with the overflow port for flowing to waste liquid cylinder;Bottom is equipped with bubble dish in aeration cylinder, is additionally provided in aeration cylinder For the water inlet pipe of conveying generation liquid into aeration cylinder and for the air inlet pipe one to bubble dish conveying gas;
Diffusion mechanism includes the gas mixer chamber set gradually from bottom to top and the gas expanded of at least one vertical stack The side wall of cylinder, gas mixer chamber is connected with gear liquid wall;The bottom of gas mixer chamber is equipped with for conveying gas to gas mixer chamber The boring aeration circle of body is connected by connecting plate with the side wall of gas mixer chamber, be communicated on boring aeration circle for its Convey the air inlet pipe two of gas;Gas expanded cylinder positioned at top is equipped with reserved thief hatch and detection mouth.
Further, the number of overflow port is 4-6.
Further, the lower part of gas mixer chamber is reverse frustoconic, and middle part is cylindrical body, and top is truncated cone-shaped.
Further, the upper end outlet of the diameter of boring aeration circle, the diameter of section of aeration cylinder and gas mixer chamber is straight Diameter is equal.
Further, the top of boring aeration circle offers stomata.
Further, pass through Sealing Method between gas mixer chamber and gas expanded cylinder and between each gas expanded cylinder Orchid is connected.
Further, the top positioned at the gas expanded cylinder at top is equipped with sealing flange cover, and sealing flange, which covers, to be equipped with Reserved thief hatch and detection mouth.
Micro-organism aerosol generating apparatus proposed by the present invention has the advantage that
Micro-organism aerosol generating apparatus proposed by the present invention includes generation mechanism and diffusion mechanism, reasonable in design, Can stablize, efficiently complete laboratory scale the microbial aerosol based on sewage (mud) generation.Whole device uses Enclosed generating device breaches in the research of field research aerosol the limitations such as influence factor is complicated, controllability is not strong;It generates The setting of aeration cylinder, bubble dish, gear liquid wall and water inlet pipe structure and positional relationship in mechanism cooperates the double-deck drum in diffusion mechanism The setting of wind aeration structure is conducive to aerosol and generates and mix diffusion, avoids conventional aerosol to greatest extent and fill It sets and the problem of liquid is repeated shearing occurs, reduce disturbance of the enclosure space interior air-flow to not aerosolized generation liquid.
Detailed description of the invention
With reference to the accompanying drawing and specific embodiment the invention will be further described.
Fig. 1 is the generating device structural schematic diagram of the embodiment of the present invention;
Fig. 2 is the generation mechanism structural schematic diagram of the embodiment of the present invention;
Fig. 3 is the generation mechanism Section A-A structural schematic diagram of the embodiment of the present invention;
Fig. 4 is the generation mechanism section B-B structural schematic diagram of the embodiment of the present invention;
Fig. 5 is the diffusion mechanism structural schematic diagram of the embodiment of the present invention;
Fig. 6 is the overlooking structure diagram of the boring aeration circle of the embodiment of the present invention and the connection relationship of gas mixer chamber;
Fig. 7 is each partial size bacterial concentration schematic diagram of the embodiment of the present invention three.
Description of symbols:
1- generation mechanism, 11- water inlet pipe, 12- air inlet pipe one, 13- bubble dish, 14- aeration cylinder, 15- overflow launder, 16- overflow Head piece, 17- waste liquid cylinder, 18- gear liquid wall, 2- diffusion mechanism, 21- air inlet pipe two, 22- boring aeration circle, 23- gas mixer chamber, 24- sealing flange, 25- gas expanded cylinder, 26- reserve thief hatch, 27- detection mouth, 28- connecting plate.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, referring to the drawings and in conjunction with specific real Example is applied, invention is further described in detail.
- Fig. 6 refering to fig. 1, the embodiment of the present invention propose a kind of closed aerosol generating device, including generation mechanism 1, position The diffusion mechanism 2 being connected above generation mechanism and with generation mechanism 1;
Generation mechanism 1 includes closing the waste liquid cylinder 17 of setting, the aeration cylinder 14 of top opening setting and positioned at aeration cylinder The gear liquid wall 18 in 14 outsides, aeration cylinder 14 and gear liquid wall 18 are respectively positioned on the top of waste liquid cylinder 17, the side wall and gear liquid of aeration cylinder 14 The overflow launder 15 of annular is formed between wall 18, the bottom of overflow launder 15 is equipped with the overflow port 16 for flowing to waste liquid cylinder 17;Aeration cylinder 14 Interior bottom is equipped with bubble dish 13, is additionally provided in aeration cylinder 14 for the water inlet pipe 11 of conveying generation liquid into aeration cylinder 14 and is used for The air inlet pipe 1 of gas is conveyed to bubble dish 13;
Diffusion mechanism 2 includes that the gas of gas mixer chamber 23 and at least one vertical stack set gradually from bottom to top expands Cylinder 25 is opened up, the side wall of gas mixer chamber 23 is connected with gear liquid wall 18;The bottom of gas mixer chamber 23 is equipped with for mixed to gas The boring aeration circle 22 that room 23 conveys gas is closed, is connected by connecting plate 28 with the side wall of gas mixer chamber 23, boring aeration The air inlet pipe 2 21 for conveying gas to it is communicated on circle 22;Gas expanded cylinder 25 positioned at top is equipped with reserved sampling Mouth 26 and detection mouth 27.
In the prior art, firstly, the research about sewage treatment plant's microbial aerosol loss feature focuses primarily upon dirt The investigation at water treatment plant scene, but for the needs of stable operation under specific operating condition, carry out generting machanism and loss characteristic aspect The controllability of research is not strong.Secondly, surrounding enviroment factor (such as temperature, humidity, wind speed, illumination etc.), sewage treatment plant itself Feature (influent quality, method of operation etc.) also has large effect to the generation of microbial aerosol and loss.It is subsequent to this Aerosol generating device, such as aerosol generating device based on bubbles burst principle are had developed, for stably generating aerosol, just In experimental study.But there is hair due to the limitation of its structure in traditional micro-organism aerosol generating apparatus based on bubbles burst The problem of raw liquid is repeated shearing, and enclosure space interior air-flow generates disturbance to not aerosolized generation liquid, influences to fill The stability set.
Based on this, the drawbacks of to overcome the field research of traditional wastewater treatment plant and traditional microbiological aerosol generating device, Microorganism is carried out suitable for the stabilization of simulated experiment, efficient Wastewater Microbial Aerosols generating device the invention discloses a kind of The research of aerosol components and existing forms, generting machanism and loss feature, can caused by avoiding field research operating condition unstable The problems such as control property is low.It is molten to generate gas by the double-deck blast aeration for closed aerosol generating device provided in an embodiment of the present invention Glue avoids or reduces generation liquid and is repeated shearing.
In embodiments of the present invention, a kind of micro-organism aerosol generating apparatus is provided.The generation that the present embodiment is previously mentioned Liquid refers to the liquid of aerosol to be generated, such as the sewage containing microorganism generated in sewage or Treatment of Sludge.That is, the present invention is real It applies in example, is that the microorganism occurred in liquid (liquid containing microorganism) is distributed in gas to generate aerosol.
Refering to fig. 1, waste liquid cylinder 17 is closing setting, and aeration cylinder 14 is located at 17 top of waste liquid cylinder.Liquid wall 18 is kept off around aeration 14 outside of cylinder, the two form annular overflow trough 15.Isopipe root be equipped with overflow port 16, aeration cylinder 14 by overflow port 16 with Waste liquid cylinder 17 communicates.From water inlet pipe 11 into aeration cylinder 14 feed liquor, make liquid occurs in aeration cylinder 14 to be in and be almost full with aeration cylinder 14 state, from air inlet pipe 1 and the air inlet into aeration cylinder via bubble dish 13.Water inlet pipe is passed through always hair into aeration cylinder Raw liquid, such as the not aerosolized extra generation liquid of liquid that occurs generate overflow, flow out aeration cylinder 14 by overflow launder 15.In addition, The liquid that entire generating device side wall stays can also be entered in waste liquid cylinder 17 by the overflow port of 15 bottom end of overflow launder.Meanwhile The bubble that boring aeration circle 22 then generates in place provides secondary driving force, further promotes gas and occurs in liquid The mixing and diffusion of microorganism generate aerosol.
In this way, it is molten to generate gas using the power that bubble dish and boring aeration circle provide the double-deck aeration in the embodiment of the present invention Glue, meanwhile, the waste liquid of generation flows to the waste liquid cylinder with aeration cylinder isolation, closing setting, and it avoids generation liquid and is repeated shearing, with And disturbance of the enclosure space interior air-flow to not aerosolized generation liquid, in this way, ensure that the stability of closed generating device.Into The aerosol of one step, generation is gradually diffused into reserved thief hatch 26 by gas expanded cylinder 25 and detection mouth 27 is detected, To complete the generation of aerosol.
In entire aerosol generating process, device is in air-tight state, overcomes existing for sewage treatment plant's field research Controllability is not strong, the problem of being easily affected by other factors, with good stability.Meanwhile it solving and being repeated because liquid occurs Shearing and enclosure space interior air-flow influence the aerosol particle object finally measured to the disturbance of not aerosolized generation liquid The problem of property, is conducive to the research to microbial aerosol component and existing forms, generting machanism and loss feature.The present invention Mainly it is adapted to the generation of microbial aerosol in sewage or sludge.
Refering to Fig. 2-Fig. 4, in generation mechanism 1, water inlet pipe 11 passes through gear liquid wall 18 and extends in aeration cylinder 14, water inlet pipe 11 Bottom end and there are gaps the bottom end of aeration cylinder 14.It is conveyed with constant flow rate to aeration cylinder 14 by water inlet pipe 11 and liquid occurs, made It obtains generation liquid in aeration cylinder 14 and is constantly in the state for being almost full with aeration cylinder 14, liquid occurs to ensure that greatest extent State and property are stablized.
Refering to Fig. 2-Fig. 3, air inlet pipe 1 passes through gear liquid wall 18, is connected by hose with bubble dish 13.Hose be convenient for into Tracheae 1 is connected with bubble dish 13, and being sent directly into aerosol generation in bubble dish 13 from the gas conveyed in air inlet pipe one makes With.
In an embodiment of the present invention, the number of overflow port 16 can be 4-6.It is specifically as follows 4,5 or 6 It is a.As shown in fig.3, the number of overflow port 16 can be 4, it can preferably avoid the generation liquid for flowing into waste liquid cylinder 17 to gas Stream has an impact.The diameter of overflow port 16 can be 15mm.
In further embodiment of this invention, 17 bottom end of waste liquid cylinder is equipped with leakage fluid dram.Leakage fluid dram is for waste liquid to be discharged.
Refering to Fig. 5, in diffusion mechanism 2,23 lower part of gas mixer chamber is reverse frustoconic, and middle part is cylindrical body, and top is circle Platform shape.The structure design of gas mixer chamber 23 may make air-flow in flow process, and sectional area generates variation, and then flow velocity generates Variation, gas flow section first become larger, and flow velocity gradually decreases, and are cylindrical body in the middle part of gas mixer chamber 23, and gas is further Mixing, 23 top of gas mixer chamber are truncated cone-shaped, and gas flow section gradually becomes smaller again, and flow velocity gradually increases, the variation of flow velocity It can promote gas adequately to mix, be conducive to the mixing and diffusion of aerosol particle.
In order to further promote to occur liquid mixing and diffusion, secondary power is provided for the generation of aerosol.In the present invention one In embodiment, boring aeration circle 22 is located at 23 bottom of gas mixer chamber, is connected with the side wall of gas mixer chamber 23 by connecting plate, The top of boring aeration circle 22 offers stomata.For example, being provided with the aperture that 6 apertures are 1mm, air-flow side on boring aeration circle 22 To be upper, dry air promotes the diffusion and mixing of aerosol particle from small hole injection.
In a preferred embodiment, the diameter of section and gas of the diameter of boring aeration circle 22, aeration cylinder 14 The upper end outlet diameter of mixing chamber 23 is equal.Aeration cylinder is tubular structure, from the point of view of fluid-mechanics point of view, more favorably Microorganism in gas and sewage is adequately mixed and is spread in this generating device, also adjustable humidity, is promoted The diffusion of gas and the microorganism in sewage in generating device.Each diameter, which is equal, is conducive to being sufficiently formed and expanding for aerosol It dissipates.
Diffusion mechanism 2 further includes air inlet pipe 2 21, and air inlet pipe 2 21 enters from 23 side wall of gas mixer chamber, with boring aeration Circle 22 is connected.Air inlet pipe 2 21 is mainly that boring aeration circle 22 provides gas source.
In an embodiment of the present invention, diffusion mechanism 2 further includes sealing flange 24, gas expanded cylinder 25 and gas mixing It is connected between room 23 and by sealing flange 24 between each gas expanded cylinder 25.
Preferably, the number of gas expanded cylinder 25 can be 1-5.Specifically, can for 1,2,3,4 or Person 5.Gas expanded cylinder 25 is mainly used for the conveying of gas in aerosol forming process.
More specifically, when gas expanded cylinder 25 is two or more, the gas expanded cylinder 25 on top is set There is sealing flange cover, sealing flange, which covers, is equipped with reserved thief hatch 26 and detection mouth 27.Reserved thief hatch 26 and detection mouth 27 are total There are five, connection pressure gauge, Hygrothermograph, gas analysis instrument etc. can be set as needed.
Below for the progress specific experiment test of generating device of the embodiment of the present invention.
Closed aerosol generating device used in following experiments can refer to Fig. 1-Fig. 6, including generation mechanism 1, is located at and generates The diffusion mechanism 2 being connected above mechanism and with generation mechanism 1;
Generation mechanism 1 includes closing the waste liquid cylinder 17 of setting, the aeration cylinder 14 of top opening setting and positioned at aeration cylinder The gear liquid wall 18 in 14 outsides, aeration cylinder 14 and gear liquid wall 18 are respectively positioned on the top of waste liquid cylinder 17, the side wall and gear liquid of aeration cylinder 14 The overflow launder 15 of annular is formed between wall 18, the bottom of overflow launder 15 is equipped with 4 overflow ports 16 for flowing to waste liquid cylinder 17;Aeration cylinder Bottom is equipped with bubble dish 13 in 14, is additionally provided in aeration cylinder 14 for the water inlet pipe 11 of conveying generation liquid into aeration cylinder 14 and is used In the air inlet pipe 1 for conveying gas to bubble dish 13;
Diffusion mechanism 2 includes the gas expanded cylinder of gas mixer chamber 23 and 3 vertical stack set gradually from bottom to top The side wall of body 25, gas mixer chamber 23 is connected with gear liquid wall 18;23 lower part of gas mixer chamber is reverse frustoconic, and middle part is cylinder Body, top are truncated cone-shaped;The bottom of gas mixer chamber 23 is equipped with the boring aeration circle for conveying gas to gas mixer chamber 23 22, it is connected by connecting plate 28 with the side wall of gas mixer chamber 23, it is the small of 1mm that 6 apertures are provided on boring aeration circle 22 Hole, airflow direction be it is upper, be communicated on boring aeration circle 22 for its convey gas air inlet pipe 2 21;Boring aeration circle 22 Diameter, the diameter of section of aeration cylinder 14, the diameter of bubble dish 13 and gas mixer chamber 23 upper end outlet diameter be equal; Gas expanded cylinder 25 positioned at top is equipped with reserved thief hatch 26 and detection mouth 27.
Embodiment one
The generation that microbial aerosol is carried out using inventive closure formula aerosol generating device selects certain using A2/O work The sewage treatment plant aeration tank sewage supernatant of skill is 200ml/min, the generation of selection as liquid, generation liquid flow of inlet water occurs Liquid liquid phase bacterium, fungi, enterobacteriaceae concentration are 7.9 × 105、5.4×104、2.1×104CFU/ml。
Generation mechanism and diffusion mechanism are passed through surrounding air, and one charge flow rate of air inlet pipe is 8.3L/min, air inlet pipe two 21 charge flow rates are 20L/min, and air inlet pipe one and 2 21 total gas flow rate of air inlet pipe are 28.3L/min, connect gas expanded cylinder Body post-sampling height is 0.5m.
The microorganism concn and grain of aerosol particle object are measured using the sampling head of the six grades of impact samplers in Anderson Diameter distribution, thief hatch flow are 28.3L/min, meet its demanding kinetics, sampling time 2min, aerosol particle object difference It strikes LB culture medium, rose bengal medium, Mai Kangkai culture medium and carries out educable bacterium, fungi, enterobacteriaceae and adopt Sample.
Experimental result is specific as follows:
Bacterial concentration at different levels is level-one (7 μm of >) 265CFU/m in the microbial aerosol of plate count measurement3, second level (4.7-7μm)71CFU/m3, three-level (4.3-4.7 μm) 88CFU/m3, level Four (2.1-3.3 μm) 141CFU/m3, Pyatyi (1.1- 2.1μm)106CFU/m3, six grades of (0.65-1.1 μm) 71CFU/m3
Fungi concentration at different levels is level-one (7 μm of >) 124CFU/m in the microbial aerosol of measurement3, second level (4.7-7 μm) 53CFU/m3, three-level (4.3-4.7 μm) 17CFU/m3, level Four (2.1-3.3 μm) 17CFU/m3, Pyatyi (1.1-2.1 μm) 35CFU/ m3, six grades of (0.65-1.1 μm) 106CFU/m3
Enterobacteriaceae concentration at different levels is level-one (7 μm of >) 0CFU/m in the microbial aerosol of measurement3, second level (4.7-7 μ m)0CFU/m3, three-level (4.3-4.7 μm) 17CFU/m3, level Four (2.1-3.3 μm) 17CFU/m3, Pyatyi (1.1-2.1 μm) 17CFU/m3, six grades of (0.65-1.1 μm) 0CFU/m3
Embodiment two
The generation of microbial aerosol is carried out using inventive closure formula aerosol generating device, selects Beijing's cell Sanitary sewage is that liquid occurs, and generation liquid flow of inlet water is 300ml/min, the generation liquid liquid phase bacterium of selection, fungi concentration 1.1 ×106、3.1×104CFU/ml。
Generation mechanism and diffusion mechanism are passed through surrounding air, and one charge flow rate of air inlet pipe is 10.3L/min, air inlet pipe two Charge flow rate is 18L/min, and air inlet pipe one and two total gas flow rate of air inlet pipe are 28.3L/min, after connecting gas expanded cylinder Height of sampling is 1.5m.
LB training is placed using the 6th grade of the sampling head (i.e. 0.65 μm of partial size >) of the six grades of impact samplers in Anderson respectively Base, rose bengal medium are supported to measure cultivable bacteria and fungi concentration in aerosol particle object, sampling time 2min, often 1h is once sampled, and carries out 10 samplings altogether.
Experimental result is specific as follows:
By plate count, after carrying out 10 samplings, bacterial concentration is respectively (unit: CFU/m in aerosol3): 602, 738,780,828,803,745,704,636,700,884;Fungi concentration is respectively (unit: CFU/m in aerosol3): 353, 309,324,306,308,387,382,353,403,405, larger change does not occur for bacterial concentration, fungi concentration, illustrates this Device has the advantages that efficient, stable.
Embodiment three
The generation of microbial aerosol is carried out using inventive closure formula aerosol generating device, selects Beijing's cell Sanitary sewage is that liquid occurs, and generation liquid flow of inlet water is 300ml/min, the generation liquid liquid phase bacterium 1.6 × 10 of selection6CFU/ ml。
Generation mechanism and diffusion mechanism are passed through surrounding air, and one charge flow rate of air inlet pipe is 10.3L/min, air inlet pipe two Total gas flow rate is 18L/min, and air inlet pipe one and two total gas flow rate of air inlet pipe are 28.3L/min, connects gas expanded cylinder Post-sampling height is 1.5m.
The microorganism concn and grain of aerosol particle object are measured using the sampling head of the six grades of impact samplers in Anderson Diameter distribution, thief hatch flow are 28.3L/min, meet its demanding kinetics, sampling time 2min, every 1h is once adopted Sample, continuous sampling is for 24 hours.Aerosol particle object strikes LB culture medium respectively, carries out the bacterium acquisition of educable each partial size.
Refering to Fig. 7, in microbial aerosol caused by sampling period generating device, wherein > 7 μm, 4.7~7 μm, 3.3 ~4.7 μm, 2.1~3.3 μm, 1.11~2.1 μm, bacterial concentration is respectively 262 ± 5CFU/ in 0.65~1.1 μm of particulate matter m3、61±4CFU/m3、90±2CFU/m3、138±6CFU/m3、102±4CFU/m3、51±4CFU/m3.It can be seen that the gas Each partial size bacterial concentration is stablized in the aerosol that colloidal sol generating device generates.
Specific embodiments of the present invention are described in detail above.Obviously, specific embodiment described herein and Attached drawing be it is exemplary and not restrictive, the scope of the present invention should not be limited by exemplary implementation, and should only by right Claim and its equivalency range are limited, and based on the case study on implementation in the present invention, those of ordinary skill in the art are not being made Under the premise of creative work, all other embodiment obtained belongs to the scope of protection of the invention.It is all according to the present patent application All the changes and improvements made by range etc., should still be within the scope of the patent of the present invention.
The above is only preferred embodiments disclosed by the invention, are not intended to limit the invention disclosure, all in the present invention Within disclosed spirit and principle, any modification, equivalent replacement, improvement and so on should be included in guarantor disclosed by the invention Within the scope of shield.

Claims (7)

1. a kind of closed aerosol generating device, it is characterised in that: including generation mechanism (1), be located at generation mechanism above and The diffusion mechanism (2) being connected with generation mechanism (1);
The generation mechanism (1) includes the waste liquid cylinder (17) for closing setting, the aeration cylinder (14) of top opening setting and is located at Gear liquid wall (18) on the outside of aeration cylinder (14), the aeration cylinder (14) and gear liquid wall (18) are respectively positioned on the top of waste liquid cylinder (17), The overflow launder (15) of annular is formed between the side wall and gear liquid wall (18) of aeration cylinder (14), the bottom of the overflow launder (15) is equipped with Flow to the overflow port (16) of waste liquid cylinder (17);Aeration cylinder (14) interior bottom is equipped with bubble dish (13), is additionally provided in aeration cylinder (14) For the water inlet pipe (11) of conveying generation liquid into aeration cylinder (14) and for the air inlet pipe one to bubble dish (13) conveying gas (12);
The diffusion mechanism (2) includes the gas of the gas mixer chamber (23) and at least one vertical stack that set gradually from bottom to top Body extends cylinder (25), and the side wall of the gas mixer chamber (23) is connected with gear liquid wall (18);The gas mixer chamber (23) Bottom is equipped with for the boring aeration circle (22) to gas mixer chamber (23) conveying gas, mixed by connecting plate (28) and gas The side wall for closing room (23) is connected, and the air inlet pipe two (21) for conveying gas to it is communicated on boring aeration circle (22);It is located at The gas expanded cylinder (25) on top is equipped with reserved thief hatch (26) and detection mouth (27).
2. closed aerosol generating device according to claim 1, it is characterised in that: the number of the overflow port (16) It is 4-6.
3. closed aerosol generating device according to claim 1, it is characterised in that: the gas mixer chamber (23) Lower part is reverse frustoconic, and middle part is cylindrical body, and top is truncated cone-shaped.
4. closed aerosol generating device according to claim 3, it is characterised in that: the boring aeration circle (22) The upper end outlet diameter of diameter, the diameter of section of aeration cylinder (14) and gas mixer chamber (23) is equal.
5. closed aerosol generating device according to claim 1, it is characterised in that: the boring aeration circle (22) Top offers stomata.
6. closed aerosol generating device according to claim 1-5, it is characterised in that: the gas mixing It is connected between room (23) and gas expanded cylinder (25) and between each gas expanded cylinder (25) by sealing flange.
7. closed aerosol generating device according to claim 6, it is characterised in that: the gas expanded cylinder positioned at top The top of body (25) is equipped with sealing flange cover, and the sealing flange, which covers, is equipped with reserved thief hatch (26) and detection mouth (27).
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CN206262506U (en) * 2016-12-13 2017-06-20 宁波工程学院 There is transmission system in a kind of fine solid particle aerosol
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