SU1364608A1 - Method of obtaining microbe cenosis for biological cleaning of waste water of coal-chemical production - Google Patents

Abstract

This invention relates to the biological treatment of industrial waste. waters of coal chemical production and concerns the biodegradation of a set of organic compounds of the same or similar classes. The aim of the invention is to enhance the functionality of the cenosis. The method consists in introducing the S composition of the activated sludge of mutant microorganisms and increasing the biomass of the microbial cenosis in two stages. At the first stage, the build-up is carried out in continuous mode on the wastewater, into which additional contaminating compounds are additionally introduced in an amount that ensures their dominance in the wastewater. The process is carried out until their oxidation is stable. At the second stage, the build-up is carried out in a periodic mode on wastewater that has undergone a primary purification from the main pollutant. They additionally introduce concomitant contaminants at a concentration corresponding to the maximum for the wastewater being treated. I il. 6

Description

The invention relates to the biological treatment of industrial wastewater from coal chemical production and concerns the biodegradation of a set of organic compounds of the same or similar classes.

The purpose of the invention is to expand the functionality of the cenosis,

Bacterial mutants, active With respect to concomitant 1F1K-organic compounds, are accumulated in the composition of the microbial cenosis, which oxidizes organic compounds of this drain.

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purification from organic matter, but additionally, the accompanying organic compound to be disposed of is introduced into it.

The drawing shows a block diagram of an installation for carrying out the method.

The installation contains a tank 1 for the nutrient medium, a dosing unit 2 for the medium, an apparatus 3 for cultivating microorganisms, a tank 4 for harvesting the crop, taken out of the apparatus by the flow of the medium, the process G4ROZODYAT in two stages. In the first - 15 mixing device 5, the mutant cells are compressed into the microcenosis of the compressing stage and are expanded by means of non-fermented cultivation on a round, simulating the waste to be cleaned, with the quality of carbon and energy sources. In this case, the concomitant compound predominates. The process is conducted until stable oxidation of the latter is obtained. At the second stage, the obtained culture in the composition of microbial cenosis is accumulated in an aeration tank in a periodic mode on the waste water 5 that has undergone primary purification from the main organic compound of the effluent being cleaned, with the additional introduction of a concurrent SE in a concentration equal to the primary load for these sewage treatment plants,

For the implementation of the method, ber. the microbial cenosis from the sewage treatment plants operating at this or similar industrial effluent, bacterial mutants into him, are capable of oxidizing certain compounds from the number accompanying the main Hot-sy and belong to the same or a close class of chemical compounds, The mixture of microorganisms is cultivated in a continuous mode.

The nutrient medium simulates the prod- uct of the waste water to be treated. It is composed of tap water with the addition of nitrogen sources, such as phosphorus and other biogenic elements. As the carbon and energy sources, the main contaminant that is discharged with waste water and the concomitant is introduced, the latter dominating in concentration. The nutrient medium can be prepared from waste water that has passed

purification from organic matter, but additionally, the accompanying organic compound to be utilized is introduced into it.

The drawing shows a block diagram of an installation for implementing the method.,.

The installation contains a tank 1 for a nutrient medium, a dosing device 2 for a medium, an apparatus 3 for cultivating microorganisms, a tank 4 for harvesting a crop, taken out of the apparatus by a flow of medium, pesor 6 air.

The installation works as follows.

The microorganisms are inoculated into a, pp. 3. With the help of a dispenser 2, nutrient medium is fed from the tank 1 into the culture apparatus 3. In tank 4, microbial biomass accumulates. In the process of continuous 25 cultivation, accumulation of mutant cells and the formation of microbial cenosis occur, stably occurring in the nutrient medium organic matter, submitted in a certain (indicated.) Practice. . .

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After the stable oxidation state of the carbon source specified in the medium is established, the mixture of microorganisms collected in the tank 4 (harvest) will be transferred to the progressed aerotank. The aeration tank is disconnected from the continuous cleaning of the waste water. The sewage water in the aeration tank undergoes primary purification from organic matter, mainly from the main pollutant of this drain. The mixture of microorganisms collected at the first stage is brought into the aeration tank, organic compounds are additionally introduced in relation to which selection and accumulation of the microorganism biomass occurs at the first stage. The concentration of this compound is set equal to the maximum concentration that is fed to the treatment plant at fluctuations and the failure of the technological regime of the main production. In the aeration tank there is an increase in the introduced mixture of microorganisms in a periodic mode. After oxidation of the fed compound, the aeration tank is switched off under load, i.e. introduced into

ordinary continuous sewage treatment

According to the proposed method, the initial conditions of operation of the microbial cenosis v under industrial conditions are such that the introduced culture, which oxidizes the accompanying compound, is maximally attainable, achieved for it in at least one aerothenic or, in the entire technological unit wastewater treatment chains, i.e. the best conditions for securing the introduced culture in these structures are provided .. 15

An example. Formation of cenosis in conditions of increased concentrations of all pollutants with the usual for the production of phenol. 20

From the wastewater of the coke-chemical production, which has been purified from phenols, there is a microbial coenosis, which purifies the effluent. Microorganisms are treated with a muta-25 gene (nitrosoguanidine). Mutated-Hbie cells and microbial cenosis make an apparatus for the continuous cultivation of microorganisms with a working volume of 0.35 liters. The nutrient medium of the DL cultivation is made up of wastewater, which is preliminarily purified from phenols, and unfinished water, taken before its dilution and containing, in a high concentration, the entire set of organic compounds that pollute the runoff.

The concentration of phenol in such a nutrient medium is 100-220 mg / l, naphthalic 50-100 mg / l. 40

After reaching the stationary state, the culture is transferred to a separately operating aeration tank, where the build-up is continued in a batch mode. Then, the azotec is loaded with 45 reduced flow of water, but with an increased concentration of all organic compounds (without dilution). The concentration of phenol at this stage is 60 mg / l, naphthalene eg 150 mg / l.

As a result, an increase in the utilization depth of phenol by 30% is noted through the month (residual concentration of volatile phenol before the introduction of the g-1.25 mg / l culture, after 0.87 mg / l). No changes in the purification of the effluent from related compounds (naphthalene, benzene, and pyridine) were noted. Cleaning process

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disrupted with an increase in the load of concomitant substances, in particular, with an increase in the concentration of naphthalene to 100 mg / ln more.

And p 2. Forked cenosis in conditions of pre & al. concomitant substances related to the compound fy - phenol.

In order to purify the effluent from the phenol compounds connected to the naphthalene series, bacteria that oxidize naphthalene are isolated from the wastewater of the coking process, and it builds them up. By treating utagene (nitrosoguanidine), mutant cells are obtained. Microbial cenosis, which is in these cleansing plants, has been transferred and the mutated cells are transferred to a laboratory for continuous growth of microorganism, as described in the example.

The build-up is carried out in two stages. At the first stage, the nutrient medium for continuous cultivation is composed of tap water, in which naphthalene in a concentration of up to 300 mg / l and phenol up to 20 mg / l are introduced. Other sources of carbon and energy are missing. Biogenic elements are introduced into the environment: nitrogen, phosphorus, alum, magnesium, sulfur in the form of salts.

After the establishment of the stationary state of oxidation of naphthalene, the second stage of the growth of microbial cenosis begins in periodic recovery; first in a 200 liter machine, then in an industrial aeration tank with work

rank of 150 m. As a nutrient medium, waste water is used, which was first purified from phenol. This water contains total phenol in concentrations up to 10 mg / l, and naphthalene in concentrations of 200 mg / l is added to it. After naphthalene has been oxidized, crystalline naphtha is introduced in the aerotank. LINE two pertsn, only 800 mg / l. The maximum concentrations of naphthalene in undiluted wastewater entering the treatment reach 800–900 mg / l.

After the oxidation of the entire naphthalene feed, the aeration tank is turned on under a nag. As a result, the level of purification with volatile phenol increases by 40% (from 26 to 0.76 mg / l), the concentration of naphthalene in the cleaned

water decreases 2.8 times (from 12.1 to 4j3 mg / l). Failures of the cleaning process in the chain have been eliminated due to load jumps on naphthalene. The latter is confirmed by the fact that, prior to the application of the proposed method for obtaining honocenosis, critical to the normal course of cleaning is the destabilizing conditions of the industrial process for the purification of phenolic wastewater.

Claims (1)

Invention Formula The method of obtaining microbial cenosis for the biological treatment of waste naphthalene concentration 100 mg / l. By increasing the waters of coal chemical production, the bath of microorganisms according to the proposed method allows to obtain microbial cenosis, which stabilizes the work of cleaning facilities during naphthalene overloads up to 800-900 mg / l. 15 The stability obtained by the predla-. The recommended mixture of microorganisms is confirmed by the stable operation of aerotanks for 20 May. In control microbiological examinations of the treatment facilities, it was established that the naphthalene utilizing culture retains its activity in the microbial cenosis of the industrial purification process. In addition, 25 culture is active against anthracene, phenanthrene, acenaphthene. providing for the introduction of mutant microorganisms into the composition of active sludge and the subsequent formation of prices by increasing the biomass of the treated wastewater, which is also distinguished by the fact that, in order to expand the functional capabilities, the price is lower. The biomass build-up was carried out in two stages, at the same time, at the first stage, the process was carried out in a continuous mode, additional accompanying compounds were introduced into the waste water in a quantity that provided their dominant concentrations, and the process was carried out until their stable oxidation, and at the second stage carried out in batch mode on the wastewaters that have undergone primary purification of the main contaminant by the active clutch, and in addition, they accompany the concentration from the concentration in the concentration corresponding to the maximum for the aetyh hundred GOVERNMENTAL water .. Thus, according to the proposed method, a microbial price is obtained, having an increased activity in relation to phenol-related organic compounds of a similar class. The price is highly stable waters of coal chemical production, 5 0 5 0 involving the introduction of mutant microorganisms into the composition of active sludge and the subsequent formation of cenoses by increasing the biomass in the treated wastewater, which is also different in that with the aim of expanding the functionality of cenosis. The biomass build-up is carried out in two stages, with the process at the first stage being carried out in a continuous mode, additionally accompanying compounds are introduced into the waste water in an amount that ensures their dominant concentrations, and the process is carried out; Until their oxidation is stable, and at the second stage, the build-up is carried out periodically on wastewater that has undergone primary purification of the main pollutant with an active clutch, while additionally introducing accompanying compounds in a concentration corresponding to the maximum for the treated wastewater. ..