SU1362478A1 - Method of obtaining blood preparation - Google Patents

Abstract

The invention relates to medicine, more specifically to the production of drugs from human blood containing albumin, ius-globulins. The purpose of the invention is to shorten the time of the process by reducing the number of technological operations. To do this, a ballast precipitate is used as a raw material, which is formed during the production of albumin from hemolyzed plasma or scatter, is treated with 15-20% ethanol, pH 4.5-5.5. Suspended precipitate after destruction of hempigments by hydrogen peroxide, the precipitate is removed The precipitated target product is ethanol at a concentration of 30-40%, pH 3.8-4.5. The drug is inactivated, subjected to clarifying and sterilizing filtration, and poured into vials. The process is shortened to 3 stages, 1.5-2 days against 5-7 days. SL

Description

The invention relates to medicine specifically to the production of drugs from human blood containing albumin, "t-and 4 /) -globulins,

The aim of the invention is to reduce the time of the process by reducing the number of technological operations.

The goal is achieved by using ballast sludge as the starting material, which is formed when albumin is obtained from hemolyzed plasma or blood serum, and treated with 15-25% ethanol at pH 4.5-5.5 suspended sediment after destruction gempigments with hydrogen peroxide, removing the precipitate by centrifuging and precipitating the target product with ethanol at a concentration of 30-40% and a pH of 3.8-4.5.

The drawing shows a diagram of the process of obtaining protein and blood product.

PRI me R 1. The ballast sediment of proteins formed during the production of albumin by the alcohol-caprilate method, in an amount of 1 kg, is poured over 6 liters of distilled water, homogenized. To the resulting suspension was added 2n. NaOH solution to pH 7.210.1, while the solution becomes dark red color. It is uniformly heated to 60 ° C and in small portions 10% hydrogen peroxide is added to an amber-colored solution, analogous to stage 3 of a known device. The solution is kept under for 2 h, cooled to and reduced to pH 5.5 1N. HCl solution. With continuous stirring, 2.5 L of 96% ethanol (to a concentration of 25%) is introduced. The temperature of the mixture is reduced to and set at these conditions for 3-4 hours. The mixture is centrifuged and the precipitate is removed. The supernatant in the amount of 9.5 l is placed in the reactor, cooled to -2 ° C and with stirring the cold ethanol is introduced in the amount of 2.4 l to a concentration of 40% and the pH of the solution is reduced to 4.5. After 3-4 hours of standing, the solution is centrifuged at a temperature not injected. The precipitate in the amount of 0.21 kg (target product) is collected, subjected to sublimation to remove residual alcohol, dissolved in distilled water to a protein concentration of 4.3–4.8%, adjusted to pH 7,, 5, inactiviC

five

0

thirty

45 50 55

Hepatitis virus is subjected to clarifying, sterilizing filtration and poured into vials.

PRI mme R 2. Ballast precipitate to proteins in an amount of 1 kg was filled with 5 liters of distilled water, homogenized and adjusted to pH 7, 1. The suspension is uniformly heated until, by the addition of hydrogen peroxide, the solution is clarified and, after 2 hours, the temperature is reduced to. Set the pH to 4.5 1N. HCl solution and ethanol are slowly added to a concentration of 15% (1.15 l total). The temperature of the mixture at the end of the precipitation reaches -3 s. The pH is corrected to 4.5 and the mixture is allowed to stand for 3-4 hours. The resulting precipitate is removed by centrifugation. The supernatant of 7.2 l is transferred to the reactor, cooled to -2 ° C, the concentration of ethanol is adjusted to 30% (1.3 l) with stirring, the pH of the solution should be 3.8. After maturing, the desired product 25 (0.16 kg) is obtained by centrifugation.

Example 5 kg of sediment is poured over 30 liters of distilled water and homogenized for 30 minutes. Without stopping stirring, increase the pH to 7.2 iO, l by the introduction of 2N. NaOH. The suspension takes on the appearance of a dark red opa- ging solution. It is uniformly heated to 60 ° C and, after reaching the desired temperature, 10% hydrogen peroxide is added in portions until it is amber. After 2 h of warming up, the solution is cooled to 1 ° C, the pH is lowered to 4.7 by the addition of 1N. HC1. 9.5 liters of chilled ethanol are introduced slowly with stirring to a final concentration of 20%,

the temperature by the end of the precipitation is reduced to support the pH of 4.7. The solution is stirred for 1 hour and after 2–3 hours, the sediment is removed by centrifugation. Next, the supernatant (42 L) is cooled to, the pH is reduced to 4.1 0.1 and 7.4 liters of cooled ethanol are slowly introduced to a concentration of 35. By the end of the precipitation, the temperature is reduced to. Checked at. Correct pH. The solution is stirred for 2-3 hours. It is possible for the mixture to settle for 10-12 hours at a temperature of -3 ° C. The precipitate is collected by centrifugation. The residual alcohol is removed by sublimation, the dry mass is dissolved in water to a protein content.

4.3–4.8% inactivate the hepatitis B virus by heating, the solution is subjected to sterilizing filtration, poured into vials and used. ,

According to the proposed method, a blood product is obtained, which is close in its properties to protein, during electrophoresis and immunophoresis, the distribution of protein zones and precipitation lines in acidic conditions is about 100% and similar to that of protein. The blood product does not contain HB antigen, bacterial impurities and pyrogenic substances, the final product contains at least 75% albumin. When using 15 scientific research institutes of extreme concentrations of alcohol, pH indicators, the final product contains albumin less than 75%.

As a result of the proposed technological scheme, the process is reduced to 20 three stages; instead of six centrifugations using a known method, only two centrifugations are necessary; the total time spent on

due to the fact that, in order to reduce the time of the process by reducing the number of technological operations, production waste is used as a source of raw material — balluminous sediment of proteins formed during the production of albumin from hemolyzed plasma or serum, while processing with ethanol is carried out the concentration of ethanol is 15–25% at a pH of 4.5–5.5 after the precipitate is suspended in distilled water and the pigments are destroyed by hydrogen peroxide;

the process is known - 25 is removed by centrifugation, and the target method is 5-7 days, and the product is precipitated with ethanol in a concentrated offered - 1.5-2 times 30-40% and a pH of 3.8 4.5.

Claims (1)

Invention Formula The method of obtaining a blood product by treating with ethanol at low temperatures the destruction of gempigments with hydrogen peroxide in the presence of sodium caprylate, precipitating the target product with ethanol at low temperatures, in order to reduce the time of the process by reducing the number of technological operations as a source of raw materials production wastes are used - ballast precipitate of proteins formed during the production of albumin from hemolyzed plasma or serum, while processing this nolom carried out in an ethanol concentration of 15-25% at pH 4.5-5.5 after suspending the precipitate in distilled water and hydrogen peroxide degradation pigments, precipitate