SU1356289A1 - Method for separating staphylococcus enterotoxin a - Google Patents

Method for separating staphylococcus enterotoxin a

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Publication number
SU1356289A1
SU1356289A1 SU3942203/13A SU3942203A SU1356289A1 SU 1356289 A1 SU1356289 A1 SU 1356289A1 SU 3942203/13 A SU3942203/13 A SU 3942203/13A SU 3942203 A SU3942203 A SU 3942203A SU 1356289 A1 SU1356289 A1 SU 1356289A1
Authority
SU
USSR - Soviet Union
Prior art keywords
column
toxin
treated
buffer solution
phosphate buffer
Prior art date
Application number
SU3942203/13A
Other languages
Russian (ru)
Inventor
Н.В. Мельников
Е.В. Бобкова
В.В. Сорочкина
Ф.С. Флуер
Original Assignee
Уфимский научно-исследовательский институт вакцин и сывороток им.И.И.Мечникова
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Уфимский научно-исследовательский институт вакцин и сывороток им.И.И.Мечникова filed Critical Уфимский научно-исследовательский институт вакцин и сывороток им.И.И.Мечникова
Priority to SU3942203/13A priority Critical patent/SU1356289A1/en
Application granted granted Critical
Publication of SU1356289A1 publication Critical patent/SU1356289A1/en

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Abstract

FIELD: medicine. SUBSTANCE: method involves concentrating culturing liquid containing the toxin on the membrane-type filters UPA-100 at 0.7-1.0 atm and flow rate of 1 l/min•cm. The so obtained concentrate is brought to pH 7.0 and treated in column having DEAE-cellulose set in equilibrium by means of 0.1 M sodium phosphate buffer solution of 7.0 pH. Then liquid containing the toxin is treated in column having KM-cellulose to separate active fractions and in column having granulated hydroxyapatite. The end product is eluted in linear gradient of 0.2-0.4 M sodium phosphate buffer solution. EFFECT: enhanced effectiveness of separation. 2 tbl
SU3942203/13A 1985-05-07 1985-05-07 Method for separating staphylococcus enterotoxin a SU1356289A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU3942203/13A SU1356289A1 (en) 1985-05-07 1985-05-07 Method for separating staphylococcus enterotoxin a

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU3942203/13A SU1356289A1 (en) 1985-05-07 1985-05-07 Method for separating staphylococcus enterotoxin a

Publications (1)

Publication Number Publication Date
SU1356289A1 true SU1356289A1 (en) 1996-03-27

Family

ID=60533523

Family Applications (1)

Application Number Title Priority Date Filing Date
SU3942203/13A SU1356289A1 (en) 1985-05-07 1985-05-07 Method for separating staphylococcus enterotoxin a

Country Status (1)

Country Link
SU (1) SU1356289A1 (en)

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