Уфимский научно-исследовательский институт вакцин и сывороток им.И.И.Мечникова
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Preparation Of Compounds By Using Micro-Organisms
(AREA)
Abstract
FIELD: medicine. SUBSTANCE: method involves concentrating culturing liquid containing the toxin on the membrane-type filters UPA-100 at 0.7-1.0 atm and flow rate of 1 l/min•cm. The so obtained concentrate is brought to pH 7.0 and treated in column having DEAE-cellulose set in equilibrium by means of 0.1 M sodium phosphate buffer solution of 7.0 pH. Then liquid containing the toxin is treated in column having KM-cellulose to separate active fractions and in column having granulated hydroxyapatite. The end product is eluted in linear gradient of 0.2-0.4 M sodium phosphate buffer solution. EFFECT: enhanced effectiveness of separation. 2 tbl
SU3942203/13A1985-05-071985-05-07Method for separating staphylococcus enterotoxin a
SU1356289A1
(en)
PROCESS FOR THE PURIFICATION OF FERMENTATION LIQUIDS CONTAINING VITAMIN B12 AND OTHER CORRINOIDS FOR PREPARING CONCENTRATES OF VITAMIN B12 OR CRYSTALLINE VITAMIN B12
Photosynthetic ATP formation from added ADP in the absence of inorganic phosphate by chromatophores from Rhodopseudomonas palustris, Rhodopseudomonas spheroides and Rhodospirillum rubrum.