SU1319800A1 - Method of accelerated propagation of meristem potato tubors - Google Patents

Abstract

The invention relates to agriculture, in particular to virus-free potato seed farming. The purpose of the invention is to reduce the growing period of the original, healthy potato material and increase the number of meris-dark tubers per test-tube plant. Potato varieties of different ripeness, improved by the method of culture of the meristem in combination with thermotherapy, are propagated by sequential cutting. Plants are freely grown in test tubes. To do this, 4–7 days prior to planting in the ground, test tubes are removed from the test tubes for a certain number of days according to the options for plant height under the plug. Plants are nourished with a Knop nutrient solution at the rate of 1-2 ml per plant. Plants are planted in a seedling manner in the field to a depth of 15-20 cm. 1 C.p. f-ly, 1 tab. (L

Description

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The invention relates to agriculture, in particular, to seed farming of potatoes on a surplus-free basis.

The aim of the invention is to reduce the growing time of the original, healthy potato material and to increase the number of meristem tubers per test-tube plant.

Example. Five varieties of potatoes of different maturity, improved by the method of culture of the meristem in combination with thermotherapy, were used in the experiment. All varieties were propagated by sequential cutting. Received 6311 plants. The plants were freely grown in test tubes in three variants. The free growing of plants consisted in the fact that tubes were removed from the tubes for a certain number of days, according to the variants, with the height of the plants under the plug. The plants were fed with a Knop nutrient solution at a rate of 1-2 ml per plant.

Experiment No. 1. For 3/4 of one day plants grew above the test tube to 3 cm.

Test No. 2. In 4-7 days, the plants grew above the test tube to 5 cm.

Test no. 3. In 10–15 days, plants grew above the test tube to 7–10 cm.

Option # 2 turned out to be optimal. The plants were strong, well developed, easily pulled out of test tubes, 35 did not break.

All plants planted 27-31 ma in field conditions (at an isotope) on an area of 0.17 ha. Landing pattern

The plant capacity was 87.3-96.5%. The whole vegetative period of the plant remained healthy (serological analysis for X, Y, S, M viruses, electron microscopic - control). After 1.5 months plants have reached a height of 20-30 cm, they began to cut, the phase of the beginning of budding; after 2 months - phase of bloom. After 3.5 months, the potatoes were harvested clones.

20

25

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The table presents the results on the quality indicators of meristem tuberous material obtained at an isotope from sample of unrooted plants in 1985.

Claims (2)

1. Method of accelerated reproduction of potato meristem tubers, including thermotherapy of tuberous material, isolation of the apical meristem from the sprouts of the original tubers, cultivation of the meristem for plant regeneration and reproduction of plants on the Murashiga-Skuga medium, characterized in that, in order to shorten the period of cultivation of the original potato material, and an increase in the number of meristem tubers per test-tube plant, well-prepared in test tubes, plants are planted in a rassadny way in the field to a depth of 15-20 cm. 2. The method according to claim 1, characterized in that the quality preparation of the plants consists in  cm. At plant height (taking into account that 4–7 days before planting in the root system) 23–25 cm of ECU, the soil of the test tubes is opened for sub-depth by 18–19 cm so that 5 6 cm. Planting plants and feeding them with nutrient solutions. ten 319800.2 The plant capacity was 87.3-96.5%. The whole vegetative period of the plant remained healthy (serological analysis for X, Y, S, M viruses, electron microscopic - control). After 1.5 months plants have reached a height of 20-30 cm, they began to cut, the phase of the beginning of budding; after 2 months - phase of bloom. After 3.5 months potatoes were harvested clones. five five 0 five 0 The table presents the results on the quality indicators of meristem tuberous material obtained at an isotope from sample of unrooted plants in 1985. Invention Formula 1. Method of accelerated reproduction of potato meristem tubers, including thermotherapy of tuberous material, isolation of the apical meristem from the sprouts of the original tubers, cultivation of the meristem for plant regeneration, and reproduction of plants on the Murahiga-Skuga medium, characterized in that, in order to shorten the period of cultivation of the original healthy potato material and increasing the number of meristem tubers per test-tube plant, well-prepared in test tubes, the plants are planted in a rassadny way in the field to a depth of 15-20 cm. 2. The method according to claim 1, characterized in that the quality preparation of the plants consists in the fact that 4-7 days prior to the landing in the ground the test tubes are opened for shchitani plants and feeding them with nutrient solutions.