SU1275008A1 - Method of biochemical removal of ethylenediamine from waste water - Google Patents

Abstract

The invention relates to microbiological purification methods and can be used in chemical industrial environment for the purification of industrial wastewater from ethylenediamine. The bacterium Bacillus sphaericus I, Micrococcus luteus 2, Bacillus circulans 3 complex is used in an equal ratio at a temperature of 18-20 ° C. The invention allows to intensify the purification process by increasing the concentration of ethylenediamine and reducing the time of the shadow oxide, as well as reducing energy waste). The invention uses new strains of bacteria. Bacillus circulans is an optional anaerobic, mobile, catalane-negative, oxidative-positive, restores nitrates to nitrites, does not form indole, starch I hydrolyzes, casein and tyrosine do not break down, gelatin does not liquefy. The optimum growth temperature is 28 ° C, pH 7.2. Ferments glucose, xylose, arabinose and mannitol without gas formation. Does not need vitamins (L. growth, not pathogenic. Micrococcus with luteus - azrob, immobile, catalase positive, does not reduce nitrates to nitrites, does not form indole, starch and gelatin does not hydrolyze. Optimum temperature of growth is 28-30 ° C, pH 7.2. Does not ferment glucose, xium, vine, arabinose, and mannitol, not pathogenic: l nen. 1 z.i, f-ly, table 2

Description

The invention relates to methods for wastewater treatment, in particular to microbiological methods, and can be used in the chemical industry for purification of industrial effluents from ethylene diamine formed, for example, in the production of ion exchange resins.

In the process of producing AN-22 brand anion exchange resin as a result of chloromethylation of ethylene diamine copolymer, wastewater containing up to 3 g / l ethylenediamine is formed at the washing stage of anion exchange resin.

The purpose of the invention is the intensification of the process with an increase in the concentration of ethylenediamine and a decrease in oxidation time, as well as a reduction in energy costs.

The method is as follows.

Wastewater with an ethylene diamine content of up to 3 g / l is preliminarily neutralized with hydrochloric acid to a pH of 6.87.3, and then treated with a complex of bacteria Bacillus sphaericus 1, Bacillus circulans 3, Micrococcus luteus 2 in a ratio of 1: 1 And at the rate of 2.5 g / l of crude biomass (0.9 g / l by dry weight) o These strains are isolated from activated sludge and adapted to ethylenediamine concentrations of up to 3 g / l, using it as the sole source of carbon and nitrogen. For normal life, potassium phosphate od- is introduced into the drain. substituted at the rate of 0.002-0.003 g / l of phosphorus.

Strain Bacillus circulans 3 ,.

Morphological signs. Tram sticks with round ends. The cell size is 2-5x0.5-0.7 microns. The spores are oval, 1.5-1.0 microns in size, located terminally, sporangia distinctly swollen.

Cultural signs. On the meat-peptone agar forms characteristic movable 'Colony ^1, large and small, flat, transparent, with a convex center, smooth, with a clearly defined edge extending from it branches new colonies.

Physiological and biochemical characteristics = Optional anaerobic, mobile, catalase-negative, oxide-positive, restores nitrates to nitrites, indole not. forms, starch. hydrolyzes, casein and tyrosine does not break down, does not dilute gelatin. The optimum temperature for growth is 28 ° C .; optimal pH of 7.2.

It ferments glucose, xylose, arabinose and mannitol without gas formation. Does not need vitamins for growth.

Not pathogenic. A geneticist was deposited at the All-Russian Research Institute under the number TsMPM V-2988.

Strain Micrococcus luteus 2.

Morphological signs. The cells are spherical 0.5-3., 5 microns in diameter, which, as a result of characteristic division, form tetrads and cubic packets, gram-positive, do not form spores.

Cultural signs. On meat and peptone agar forms colonies with a diameter of · 20 1-2 mm, round, with smooth edges, convex, pasty consistency, matte, yellow. On the meat-peptone broth forms a wall ring, flocculent ²⁵ sediment. the environment remains transparent.

Physiological and biochemical characteristics. Aerobic, motionless, catalase-positive, does not reduce nitrates to nitrites, does not form indole, starch30 is small and gelatin does not hydrolyze. The optimum temperature for growth is 28-30 C, the optimal pH of the medium is 7.2.

Does not ferment glucose, xylose, arabinose and mannitol.

Not pathogenic. A geneticist was deposited at the All-Russian Research Institute under the number TsMPM B-2989.

PRI me R 1 Wastewater with an ethylene diamine content of up to 3 g / l is neutralized with hydrochloric acid, up to 40 pH is adjusted to 6.8-7.3.

Wastewater is supplied at a rate of 1 l / day to a laboratory model wastewater treatment plant, which is an aerotank mixture45 filled with a fiberglass load, which is treated with a complex of bacteria Bacillus sphaericus I, Bacillus circulans 3 and Micrococcus luteus 2 in an equal ratio of 50 based on 2.5 g / l of crude biomass (O, 9 g / l by dry weight). As a biogenic element, potassium phosphate monosubstituted is introduced at the rate of 0.002-0.003 g / l of phosphorus.

The process is carried out at 18-20 ° C. The residence time of water in the installation for the complete decomposition of eth.pgdiamine is 36 hours, the degree of purification is 100%.

1275008 4

The determination of ethylene diamine is carried out by the photometric method.

The test results are given in table. 1.

Example 2 o The process is 5 as in example 1, but at 23 ° C.

Comparative results of examples and 2 are given in table. 2.

As can be seen from the table. 2, the degree of purification from ethylene diamine does not decrease — ¹⁰ with decreasing temperature and in both cases is 100%,

Example 3. In the conditions of example 1, wastewater is treated with a complex of bacteria in a ratio of 2: 1: 1. ^ The degree of purification of wastewater from ethylenediamine is 100%.

When processing the complex of the proposed bacteria in a ratio of 1: 1: 2 20, incomplete destruction of ethylenediamine is observed, its biodegradation occurs at 32%.

When treated with a complex of bacteria in a ratio of 1: 1: 0.5, ethylene diamine is destroyed by 48%.

Claims (2)

t The invention relates to wastewater treatment methods, in particular to microbiological methods, and can be used in high quality industrial wastewater treatment from ethylenediamine 5 formed, for example, with -hpO-KSBOACTBe ion exchange resins. In the process of obtaining the Acionite of the mark AN-22 as a result of chloromethylation of an ethylenediamine / mine copolymer at the stage of washing the anion exchanger, wastewater containing up to 3 g / l of ethylenediamine. costs. The method is carried out in the following way. Wastewater containing ethylene diamine, up to 3 g / l is preliminarily neutralized with hydrochloric acid to pH 6.8 7.3, and then treated with the bacterial complex Bacillus sphaerlcus I, Bacillus circulans 3f Mlcrococcus luteus 2 in the ratio of 1s and 1 based on 2.5 g / l raw biomass (0.9 g / l dry weight) Said gotamm is derived from activated sludge and adapted to an ethylenediamine concentration of up to 3 g / l using it as the sole carbon and nitrogen source. For normal livelihood potassium phosphate of monosubstituted is added to the stock at the rate of 0.002 0, 003 g / l of phosphorus. Strain Bacillus circulans 3, Morphological features, Gramvariant sticks with round ends. The cell size is 2-5x0.5-0.7 microns. Ssora oval sizes of 1.3-0.0 microns are terminally located, the sporangium is distinctly bloated. Cultural signs. On a metro agar forms characteristic mobile colonies (large, small, flat, transparent, about a convex center, a smooth, clearly enveloped edge with new branches of branches, Physiological and biochemical features, Optional anaerobic catalo-negative, with a negative nodule), Physiological and biochemical signs, Optional anaerobes, catalo-negative, suitable for catalysis positive, reduces nitrates to nitrites, indole does not form starch, hydrolyzes, casein and tyrosine ke 8 splits, gelatin does not liquefy.The optimum temperature for growth is 28 ° C, a) optimum pH, ferments glucose, xylose, arab foreignness and mannitol without gas formation. Not, aaetsl in vitamins for growth o Not pathogenic. It is deposited in the All-Russian Scientific-Research Institute of Genetics under the number CMSH B-2988, Strain Micrococciis luteus 2, Morphological features. Spherical cells Oe 5–3 ,, 5 µm in diameter, which as a result of the characteristic division of the etrade order and cubic packets of gram-positive, do not form a spore, Cultural characteristics. On m, septon agar forms colonies with a diameter of 1-2 mm, round with smooth edges, convex, pasty consistency, matt, yellow. On mi-peptone broth forms a wall ring, flocculent sediment. the medium remains transparent. Physiological and Bioch :: - 1 physical signs. Aerobe, immobile, catalase-positive, does not reduce nitrates to nitrites, I1-SCHOL: n: e forms, starch and gelatin do not hydrolyze. The optimum temperature for growth is 28–30 ° C and the optimum pH of the medium is 7.2. Does not ferment glucose, xylose, arabinose n mannitol. Not pathogenic. It is deposited in the All-Russian Scientific-Research Institute of Genetics under the nome: rum CMPM B-2989. P i d e R 1 Waste water with an ethylenediamine content of up to 3 g / l neutralized hydrochloric acid to a pH of up to 6, 8-7.3. Sewage water is fed at a rate of 1 l / day to a laboratory model installation DO7Y г Sewage whistles, is an aeration tank, filled with fiberglass loading by KOTopyjn, treated with IBacillus sphaericus 15 bacterium Bacillus eirculans 3 and Micrococcus lutsus 2 with a ratio of 3 2 ,, 5 g / l raw biomass 0j9 g / l on a dry1 "g. weight). Phosphorous potassium monosubstituted at the rate of 05002-0-0.003 g / l of phosphorus is introduced as a booster. The process is carried out at 18-20 seconds. The residence time of the water in the unit for the decomposition of E1 pendiamine is 36 h, the degree of purification I) 0l. The determination of ethylene diamine is carried out by photometric method. The test results are shown in Table. 1. Example 2 o The process is carried out analogously to example 1, but with. The comparative results of examples 1 and 2 are given in Table 2. As can be seen from Table 2, the degree of purification from ethylene diamine does not decrease with decreasing temperature and in both cases is 100%. Example 3. Under the conditions of Example 1, wastewater is treated with a complex of bacteria in a ratio of 2: 1: 1. The degree of purification of ethylenediamine from wastewater is 100%. When processing the proposed bacteria in the 1: 1: 2 ratio, ethylenediamine destruction is incomplete, its biodegradation occurs by 32%. When treated with a bacterial complex in the ratio of 1: 1: 0.5, the destruction of ethylene diamine occurs by 48%. Claim 1. The method of biochemical treatment of wastewater from ethylene namin, including the use of a strain of Bacillus sphaericus I, characterized in that, in order to enhance the process with increasing Concentrations of ethylene diamine and reducing the time of oxidation, as well as reducing energy costs, are additionally used by bacteria - destructors of ethylenediamine Micrococcus luteus 2 and Bacillus circulans 3 in equal proportions, 2. A method according to claim 1, characterized in that the process is conducted at 18--20 ° C. Table 1 Offered way 0.7-0.9 3.0O 0.7-0.9 3.0O 0.98 2.2824 1.1 1.5648 , 26 O100 Isayst way 1.25 0.7575 table 2