SU1258367A1 - Method of indicating bacteria - Google Patents

Description

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The invention relates to medicine, primarily microbiology, and can be used in the indication of bacteria.

The purpose of the invention is to increase the sensitivity of the method by introducing into the culture bacteriophage mixed with lysozyme.

Example. Any of the used polyvalent bacteriophages, whole or developed. 10 (Salmonella, Proteus, Pseudomuscular, Staphylococcal) mixed in a ratio of 1: 1

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with the preparation of egg lysozyme in concentration of 100 μg / ml. An official preparation of lysozyme from chicken egg protein is used.,

As can be seen from the table, the use of polyvalent bacteriophages in combination with lysozyme for diagnostic purposes allows an almost 3-fold improvement in the interpretation of atypical strains of microorganisms used from patients.

This method eliminates the need to carry out long, multi-day passages of atypical cultures, which require a large consumption of nutrient media, to achieve reversion to the original typical forms and allows you to order in 50% of cases 5050/2

Pro.v.polygres pr-tie, Uzhgorod, st. Project, 4

and

2583672

The selected atypical strains of microorganisms (proteins in 0-form or non-agglutinable; sine-neurosis-stick in bottomless {alternatively, etc.) are sown with a continuous lawn daily broth culture on the surface of the mi-peptone agar. Excess culture is drained, the cup is dried in a thermostat for 20 minutes. Then, 10 a mixture of lysozyme with a bacteriophage is applied to it, with respect to which the culture is identified.

The cup is placed in a thermostat at 18 o'clock. Records are kept in zones 15 of microbial culture lysis.

The results of the diagnostic application of the combination of polyvalent bacteriophage with lysozyme are presented in the table.

to determine the species identity of the aforementioned already after 18 h from the moment of obtaining a pure culture.

The use of lysozyme in combination with a bacteriophage increased the percentage of protyped salmonella cultures by 11.0%, prote - by 16.3%, blue pus - by 23.8%, and staphylococcus - by 28.6%.

The application of the proposed method will improve the quality of diagnosis of infectious diseases, to achieve savings in nutrient media and working time.

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Claims (1)

METHOD FOR INDICATING BACTERIA by introducing a specific bacteriophage and biologically active substance into the bacterial culture, followed by taking into account the culture lysis zones, characterized in that, in order to increase the sensitivity of the method, lysozyme is used as a biologically active substance at a concentration of 100 μg / ml and higher at a ratio a solution of lysozyme and a suspension of bacteriophage 1: 1. Joint venture G0 O) m>