SU1223913A1 - Method of obtaining galactosidase - Google Patents
Method of obtaining galactosidase Download PDFInfo
- Publication number
- SU1223913A1 SU1223913A1 SU833677888A SU3677888A SU1223913A1 SU 1223913 A1 SU1223913 A1 SU 1223913A1 SU 833677888 A SU833677888 A SU 833677888A SU 3677888 A SU3677888 A SU 3677888A SU 1223913 A1 SU1223913 A1 SU 1223913A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- galactosidase
- obtaining
- homogenization
- elution
- leaves
- Prior art date
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Description
Изобретение относитс к органической химии, в частности к биохимии, и может быть использовано дл лабораторного и промышленного получени jb-галактозидазы и перок- сидазы из полыни.The invention relates to organic chemistry, in particular biochemistry, and can be used for laboratory and industrial production of jb-galactosidase and peroxidase from wormwood.
Цель изобретени - дополнительное получение пероксидазы и повышение стабильности целевого продукта.The purpose of the invention is the additional production of peroxidase and an increase in the stability of the target product.
Пример. 10 г стеблей, листьев и соцветий полыни гомогенизируют в дистиллированной воде с 0,6 М NaCl и отжимают черезExample. 10 g of stems, leaves and inflorescences of wormwood are homogenized in distilled water with 0.6 M NaCl and squeezed through
ткань. Балластные белки осаждают сульфатом аммони до 20% насыщени . Осадок отбрасывают, а в надосадочную жидкость добавл ют сульфат аммони до насыщени . Осадок отцентрифугируют, перераствор ют в дистиллированной воде и пропускают через колонку с 6-50. Получают белковый экстракт 24 мг, характеризующийс пероксидазной активностью 560 мкмоль/ /мин на 1 мг белка и i -галактозидазной 20,6 мкмоль/мин на 1 мг белка.the cloth. Ballast proteins are precipitated with ammonium sulfate to 20% saturation. The precipitate is discarded, and ammonium sulfate is added to the supernatant until saturation. The precipitate is centrifuged, redissolved in distilled water and passed through a column of 6-50. A 24 mg protein extract is obtained, characterized by a peroxidase activity of 560 µmol / min for 1 mg of protein and i β-galactosidase 20.6 µmol / min for 1 mg of protein.
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU833677888A SU1223913A1 (en) | 1983-12-23 | 1983-12-23 | Method of obtaining galactosidase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU833677888A SU1223913A1 (en) | 1983-12-23 | 1983-12-23 | Method of obtaining galactosidase |
Publications (1)
Publication Number | Publication Date |
---|---|
SU1223913A1 true SU1223913A1 (en) | 1986-04-15 |
Family
ID=21094831
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU833677888A SU1223913A1 (en) | 1983-12-23 | 1983-12-23 | Method of obtaining galactosidase |
Country Status (1)
Country | Link |
---|---|
SU (1) | SU1223913A1 (en) |
-
1983
- 1983-12-23 SU SU833677888A patent/SU1223913A1/en active
Non-Patent Citations (1)
Title |
---|
Белецкий Ю. Д. Генетика, 1978, т. 14, с. 1387-1392. * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE195148T1 (en) | PROCESS FOR THE RECOMBINANT PRODUCTION OF HIV-2 PROTEINS AND CELLS THAT EXPRESS HIV-2 PROTEINS | |
ATE110782T1 (en) | RECOMBINANT HUMAN ENDOTHELIAL CELL GROWTH FACTOR. | |
PT93910A (en) | A product for the preparation of a blood protein by means of a blood cell | |
ES8104412A1 (en) | Process for the preparation of a purified protein hydrolysate | |
ATE65250T1 (en) | PROCESSES FOR THE MANUFACTURE OF VERY PURE DERMATAN SULFATE AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM. | |
ES8503238A1 (en) | Process for the preparation of protein concentrates and nutriments by processing animal blood | |
DE3230151A1 (en) | NEW POLYPEPTIDE WITH EFFECT ON THE IMMUNE SYSTEM, METHOD FOR ITS INSULATION AND CLEANING, ITS USE, ITS CONTAINER, AND ITS DIFFERENT PRODUCTS, THEIR USE AND ITEMS | |
SU1223913A1 (en) | Method of obtaining galactosidase | |
DK164916C (en) | PROCEDURE FOR CLEANING OR ENHANCING BIOLOGICAL ACTIVE PROTEINS AND SUITABLE AGENTS | |
RU97115965A (en) | METHOD FOR COMPREHENSIVE PROCESSING OF CHITIN-CONTAINING RAW MATERIALS | |
SU535912A3 (en) | Method of producing orgotein | |
SU1108102A1 (en) | Method of obtaining peroxidase | |
DE3786870T2 (en) | Method of making proteins. | |
SU411867A1 (en) | ||
SU575353A1 (en) | Method of obtaining carboxypepiidase | |
RU1836957C (en) | Method of trophoblastic beta-1-glycoprotein preparing | |
RU94043698A (en) | METHOD OF PREPARING ENZYME DESTABILASE SHOWING FIBRINOLYTIC, THROMBOLYTIC, ENDO- AND EXO-ε (γ-glu)-LYS-ISOPEPTIDASE AND AMIDOLYTIC ACTIVITY | |
SU1280546A1 (en) | Method of producing leukocytic beta sub 1 - glycoproteide | |
RU97121168A (en) | METHOD FOR CHROMATOGRAPHIC ISOLATION AND CLEANING OF PROTEINS, PEPTIDES AND THEIR COMPLEXES | |
SU459475A2 (en) | Purification Method - Asparaginase | |
KR900000384A (en) | Method for Purifying Human γ-Interferon Expressed in Yeast | |
RU1836954C (en) | Method of preparing immunomodulating peptides | |
Ruscha et al. | A simple procedure for the isolation of brain actin | |
GB1367534A (en) | Process for the treatment of waters containing proteins | |
RU2077537C1 (en) | Method for production of ovomucoid |