SU1223913A1 - Method of obtaining galactosidase - Google Patents

Method of obtaining galactosidase Download PDF

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Publication number
SU1223913A1
SU1223913A1 SU833677888A SU3677888A SU1223913A1 SU 1223913 A1 SU1223913 A1 SU 1223913A1 SU 833677888 A SU833677888 A SU 833677888A SU 3677888 A SU3677888 A SU 3677888A SU 1223913 A1 SU1223913 A1 SU 1223913A1
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SU
USSR - Soviet Union
Prior art keywords
galactosidase
obtaining
homogenization
elution
leaves
Prior art date
Application number
SU833677888A
Other languages
Russian (ru)
Inventor
Бектас Гафурович Мухамеджанов
Рауза Минлиахмедовна Кунаева
Наталья Салимовна Магазова
Константин Васильевич Лопатин
Original Assignee
Институт молекулярной биологии и биохимии АН КазССР
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Application filed by Институт молекулярной биологии и биохимии АН КазССР filed Critical Институт молекулярной биологии и биохимии АН КазССР
Priority to SU833677888A priority Critical patent/SU1223913A1/en
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Publication of SU1223913A1 publication Critical patent/SU1223913A1/en

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Description

Изобретение относитс  к органической химии, в частности к биохимии, и может быть использовано дл  лабораторного и промышленного получени  jb-галактозидазы и перок- сидазы из полыни.The invention relates to organic chemistry, in particular biochemistry, and can be used for laboratory and industrial production of jb-galactosidase and peroxidase from wormwood.

Цель изобретени  - дополнительное получение пероксидазы и повышение стабильности целевого продукта.The purpose of the invention is the additional production of peroxidase and an increase in the stability of the target product.

Пример. 10 г стеблей, листьев и соцветий полыни гомогенизируют в дистиллированной воде с 0,6 М NaCl и отжимают черезExample. 10 g of stems, leaves and inflorescences of wormwood are homogenized in distilled water with 0.6 M NaCl and squeezed through

ткань. Балластные белки осаждают сульфатом аммони  до 20% насыщени . Осадок отбрасывают, а в надосадочную жидкость добавл ют сульфат аммони  до насыщени . Осадок отцентрифугируют, перераствор ют в дистиллированной воде и пропускают через колонку с 6-50. Получают белковый экстракт 24 мг, характеризующийс  пероксидазной активностью 560 мкмоль/ /мин на 1 мг белка и i -галактозидазной 20,6 мкмоль/мин на 1 мг белка.the cloth. Ballast proteins are precipitated with ammonium sulfate to 20% saturation. The precipitate is discarded, and ammonium sulfate is added to the supernatant until saturation. The precipitate is centrifuged, redissolved in distilled water and passed through a column of 6-50. A 24 mg protein extract is obtained, characterized by a peroxidase activity of 560 µmol / min for 1 mg of protein and i β-galactosidase 20.6 µmol / min for 1 mg of protein.

Claims (1)

СПОСОБ ПОЛУЧЕНИЯ ^-ГАЛАКТОЗИДАЗЫ путем гомогенизации листьев растений, экстракции, фракционного высаливания сульфатом аммония и гельфильтрации на сефадоксе G-50 с последующей элюцией, отличающийся тем, что, с целью дополнительного получения пероксидазы и повышения стабильности целевого продукта, в качестве источника сырья используют листья полыни, а для гомогенизации и элюции — 0,58—0,62 М раствор хлористого натрия.METHOD FOR PRODUCING ^ -GALACTOSIDASE by homogenization of plant leaves, extraction, fractional salting out with ammonium sulfate and gel filtration on Sephadox G-50 followed by elution, characterized in that, in order to obtain additional peroxidase and increase the stability of the target product, wormwood leaves are used as a source of raw materials , and for homogenization and elution - 0.58-0.62 M sodium chloride solution. (Л G ю ю ос с©(L G y s os s © ОС >OS>
SU833677888A 1983-12-23 1983-12-23 Method of obtaining galactosidase SU1223913A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU833677888A SU1223913A1 (en) 1983-12-23 1983-12-23 Method of obtaining galactosidase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU833677888A SU1223913A1 (en) 1983-12-23 1983-12-23 Method of obtaining galactosidase

Publications (1)

Publication Number Publication Date
SU1223913A1 true SU1223913A1 (en) 1986-04-15

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
SU833677888A SU1223913A1 (en) 1983-12-23 1983-12-23 Method of obtaining galactosidase

Country Status (1)

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SU (1) SU1223913A1 (en)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Белецкий Ю. Д. Генетика, 1978, т. 14, с. 1387-1392. *

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