KR900000384A - Method for Purifying Human γ-Interferon Expressed in Yeast - Google Patents

Method for Purifying Human γ-Interferon Expressed in Yeast Download PDF

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Publication number
KR900000384A
KR900000384A KR1019880007647A KR880007647A KR900000384A KR 900000384 A KR900000384 A KR 900000384A KR 1019880007647 A KR1019880007647 A KR 1019880007647A KR 880007647 A KR880007647 A KR 880007647A KR 900000384 A KR900000384 A KR 900000384A
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South Korea
Prior art keywords
interferon
human
yeast
purifying
cys
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KR1019880007647A
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Korean (ko)
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KR900008571B1 (en
Inventor
임관열
송영호
한규범
정현호
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허신구
주식회사 럭키
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Priority to KR1019880007647A priority Critical patent/KR900008571B1/en
Publication of KR900000384A publication Critical patent/KR900000384A/en
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Publication of KR900008571B1 publication Critical patent/KR900008571B1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음No content

Description

효모에서 발현된 인체 γ-인터페론의 정제방법Method for Purifying Human γ-Interferon Expressed in Yeast

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 143개의 아미노산으로 구성된 γ-인터페론의 아미노산 서열을 나타내고,1 shows the amino acid sequence of γ-interferon consisting of 143 amino acids,

제2도는 본 발명의 정제 과정에 따른 단계별 중간생성물 및 최종생성물을 15% 소디움도데실설페이트-폴리아크릴아마이드 겔 전기영동(SDS-PAGE)한 결과를 나타내며,2 shows the results of 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of intermediate and final products according to the purification process of the present invention.

제3도는 제2도의 정제과정에 의해 정제된 최종 γ-인터페론을 덴시토미터 스케닝(Densitometer Scanning)한 결과를 나타낸다.FIG. 3 shows the results of Densitometer Scanning of the final γ-interferon purified by the purification process of FIG. 2.

Claims (2)

효모로부터 인체 γ-인터페론을 분리 정제하는데 있어서, 인체 γ-인터페론이 발현되어 있는 효모를 PMSF(phenylmethyl sulfony fluoride)가 함유된 완충용액에 현탁 및 분쇄시킨 후 세포잔해물 및 가용성물질을 제거하고 난 불용성 단백질을 요소가 함유된 완충용액에 용해시키고 이로부터 불용성 물질을 제거한 다음, 그 상등액인 단백질 용액을 양이온 교환 크로마토그라피하여 γ-인터페론을 조정제시키고, 조정제된 인체 γ-인터페론을 겔 여과 크로마토그라피하여 95%이상의 순도로 더욱 정제시킨후, 정제된 인체γ-인터페론을 트리에틸아민/초산 혼합물에 혼합교반시키고 아세톤으로 침전시킨후 침전물을 생리포도당에 용해시키고 이를 용매투석시키는 것을 특징으로 하는 효모에서 발현된 인체 γ-인터페론의 정제방법.In separating and purifying the human γ-interferon from yeast, the yeast expressing the human γ-interferon is suspended and pulverized in a buffer solution containing phenylmethyl sulfony fluoride (PMSF), and then the cellular debris and soluble substances are removed. Was dissolved in a buffer solution containing urea and insoluble matter was removed therefrom, and the supernatant protein solution was subjected to cation exchange chromatography to adjust γ-interferon, and the adjusted human γ-interferon was gel filtered by 95%. After further purification with the above purity, the purified human γ-interferon was mixed and stirred in triethylamine / acetic acid mixture and precipitated with acetone, and then the precipitate was dissolved in physiological glucose and solvent dialysis. Method for Purifying γ-Interferon. 제1항에 있어서, 인체 γ-인터페론의 아미노산 서열은 그 N-말단이 시스테인-티로신-시스테인(Cys-Tyr-Cys)이 없는 글루타민(Gln)으로 시작되는 것을 특징으로 하는 방법.The method of claim 1 wherein the amino acid sequence of the human γ-interferon is characterized in that its N-terminus begins with glutamine (Gln) free of cysteine-tyrosine-cysteine (Cys-Tyr-Cys). ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019880007647A 1988-06-24 1988-06-24 Method for purification of ???-interferon KR900008571B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019880007647A KR900008571B1 (en) 1988-06-24 1988-06-24 Method for purification of ???-interferon

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019880007647A KR900008571B1 (en) 1988-06-24 1988-06-24 Method for purification of ???-interferon

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KR900000384A true KR900000384A (en) 1990-01-30
KR900008571B1 KR900008571B1 (en) 1990-11-24

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100481976B1 (en) * 2002-02-21 2005-04-13 박석균 Composite material of high tenacity and non-retraction grouting material which including powder of wasted tire and powder of plastic

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101984104B1 (en) * 2016-08-24 2019-05-30 국방과학연구소 Dpdt relay based pyro driving circuit

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100481976B1 (en) * 2002-02-21 2005-04-13 박석균 Composite material of high tenacity and non-retraction grouting material which including powder of wasted tire and powder of plastic

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Publication number Publication date
KR900008571B1 (en) 1990-11-24

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