SU1218927A3 - Method of processing vegetable raw material for producing biomass of fodder yeast and/or ethyl alcohol - Google Patents

Method of processing vegetable raw material for producing biomass of fodder yeast and/or ethyl alcohol Download PDF

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SU1218927A3
SU1218927A3 SU813373695A SU3373695A SU1218927A3 SU 1218927 A3 SU1218927 A3 SU 1218927A3 SU 813373695 A SU813373695 A SU 813373695A SU 3373695 A SU3373695 A SU 3373695A SU 1218927 A3 SU1218927 A3 SU 1218927A3
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yeast
ethyl alcohol
raw material
fodder yeast
biomass
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SU813373695A
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Russian (ru)
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Надь Дьюла
Керекеш Роберт
Шомодьи Пал
Резешши Юдит
Болдижар Вайда
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Йозеф Аттила Мг Тсз,Внр (Инопредприятие)
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/22Processes using, or culture media containing, cellulose or hydrolysates thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/08Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Animal Husbandry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)

Abstract

A cellulose-containing starting substance is pre-treated with a dilute mineral acid and/or a dilute base, and then fermented under aerobic or anaerobic conditions in the presence of a Candida utilis var. cellulolytica strain deposited at the Hungarian National Collection of Microorganisms of the National Institute for Public Health on 23rd September, 1980 under No. CU 28 00199. When fermentation is performed under aerobic conditions, fodder yeast or a mixture of fodder yeast and ethanol is obtained, depending on the aeration rate, whereas when fermentation is performed under anaerobic conditions, ethanol is obtained.

Description

«121"121

Изобретение относитс  к биотехнологии ,- а именно к способу переработки растительного сырь  дл  получени  биомассы кормовьк дрожжей и/или этилового спирта.The invention relates to biotechnology, namely to a method for processing plant raw materials to obtain biomass of feed yeast and / or ethyl alcohol.

Цель изобретени  - упрощение способа, расширение сырьевой базы и уменьшение энергозатрат.The purpose of the invention is to simplify the method, expanding the raw material base and reducing energy consumption.

Способ осуществл ют следующим образом.The method is carried out as follows.

Растительные отходы предварительно обрабатывают разбавленными растворами кислоты или щелочи, а затем их используют в качестве субстрата дл  выращивани  кормовых дрожжей Candida utilis var. oellulolyti- ca № CU 2800199.Plant waste is pretreated with dilute solutions of acid or alkali, and then used as a substrate for growing fodder yeast Candida utilis var. oellulolyti- ca № CU 2800199.

Штамм хранитс  в Венгерской национальной коллекции штаммов и имеет следующую характеристику.The strain is stored in the Hungarian National Collection of Strains and has the following characteristic.

Клетки эллипсоидной формы, меньший диаметр 2,5-4,5 мкм, больший диаметр 4,5-7,5 мкм. Образует белые, выпуклые колонии с блест щей поверхностью и гладкими кра ми.Cells of ellipsoidal shape, smaller diameter 2.5-4.5 microns, larger diameter 4.5-7.5 microns. It forms white, convex colonies with a shiny surface and smooth edges.

Штамм усва.ивает глюкозу, сахарозу , мальтозу, целлобиозу, целлодек- стрин, рафинозу, инулин.The strain assimilates glucose, sucrose, maltose, cellobiose, cellodextrin, raffinose, inulin.

Штамм расщепл ет глюкозу, сахарозу , рафинозу, ц ёллобиозу, целло- декстрин.The strain decomposes glucose, sucrose, raffinose, cellobiosis, cellodextrin.

Штамм  вл етс  сапрофитом, токсинов не вырабатьшает. Оптимальна  температура роста 37 С. При выращивании штамма в анаэробных услови х получают этиловый спирт, при подаче 1,0-4,0 мг Oj /л получают биомассу дрожжей , а при подаче 0,1-0,8 мг 02/л - биомассу и этиловый спирт одновременно .The strain is a saprophyte, toxins do not work out. The optimal growth temperature is 37 ° C. Ethanol is obtained when the strain is grown under anaerobic conditions, yeast biomass is obtained when feeding 1.0-4.0 mg Oj / l, and biomass is fed at 0.1-0.8 mg 02 / l and ethyl alcohol at the same time.

Пример 1. 1000 вес.ч. кенафа размалывают на молотковой мельнице до размера частиц 1-2 мм. К размолотому сырью добавл ют 9000 вес.ч. 3,2%-ной серной кислоты и подвергают тепловой обработке в кислотоупорном резервуаре в течение 120 мин при 96 С. В полученной смеси раствор ют 7,2 вес.ч. суперфосфата и 4,0 вес.ч. сульфата аммони , после чего осадок отдел ют центрифугированием . В раствор добавл ют гидроокись натри  до значени  рН 6,5, охлаждают до З7 с и в соотношении 1:10 инокулируют раствором, содержащим 10 клеток/мл культуры Candida utilis var. cellulolytica № CU 2800199.Example 1. 1000 weight.h. kenaf milled in a hammer mill to a particle size of 1-2 mm. 9000 parts by weight are added to the milled feed. 3.2% sulfuric acid and subjected to heat treatment in an acid-resistant tank for 120 minutes at 96 ° C. In the resulting mixture, 7.2 parts by weight are dissolved. superphosphate and 4.0 wt.h. ammonium sulfate, after which the precipitate is separated by centrifugation. Sodium hydroxide is added to the solution to a pH value of 6.5, cooled to 37 ° C and inoculated at a ratio of 1:10 with a solution containing 10 cells / ml of the culture of Candida utilis var. cellulolytica No. CU 2800199.

Культивирование осуществл ют при концентрации кислорода 1 мг/л. ,The cultivation is carried out at an oxygen concentration of 1 mg / l. ,

7272

После окончани  культивировани  дрожжи отдел ют и сушат. Из 100 кг кенафа получают I6 кг сухих кормовых дрожжей.After completion of the culture, the yeast is separated and dried. From 100 kg of kenaf, I6 kg of dry fodder yeast is obtained.

Пример2. Способ осуществл ют согласно примеру 1, но в качестве сырь  используют, отходы бумаги из сульфатной целлюлозы. Предварительную обработку провод тExample2. The method is carried out according to Example 1, but as a raw material, waste paper from Kraft pulp is used. Pre-processing is carried out

10%-ной серной кислотой при 80 с в течение 40 мин. Суспензию фильтруют , твердое вещество обрабатывают 0,5%-ным раствором гидроокиси натри  при 100 С в течение 25 мин.10% sulfuric acid at 80 s for 40 min. The suspension is filtered, the solid is treated with 0.5% sodium hydroxide solution at 100 ° C. for 25 minutes.

После фильтрации обе жидкие фазы смешивают. Культивирование осуществл ют при концентрации кислорода О,1 мг/л. Перегонкой культураль- ной жидкости получают этиловый, спирт.After filtration, both liquid phases are mixed. The cultivation is carried out at an oxygen concentration of 0 mg / l. Ethyl alcohol is obtained by distillation of the culture fluid.

Из 100 кг бумаги получают 15,5 л абсолютного спирта и 1,5 кг дрожжей.From 100 kg of paper receive 15.5 liters of absolute alcohol and 1.5 kg of yeast.

П р и м е р 3. Способ осуществл ют согласно примеру 1, но в качестве сырь  используют листву сахарной свеклы. Предварительную обработку ведут 0,5%-ной серной кислотой при 100°С в течение 35 мин. Культивирование осуществл ют при рН 6,3 в течение 42 ч при концентрации кислорода 4 мг/л. Из 100 кг истьев получают I3 кг абсолютно сухих дрожжей.EXAMPLE 3. The method is carried out according to Example 1, but sugar beet leaves are used as raw materials. Pre-treatment is conducted with 0.5% sulfuric acid at 100 ° C for 35 minutes. The cultivation is carried out at a pH of 6.3 for 42 hours at an oxygen concentration of 4 mg / l. Out of 100 kg of waste, I3 kg of absolutely dry yeast is obtained.

П р и м е р 4. Способ осуществл ют согласно примеру 1, но в качестве сырь  используют опилки, которые обрабатьшают 11,0%-ным раствором гидроокиси натри  при 80°С в течение 45 мин. После культивировани  дрожжей в анаэробных услови х в течение 52 ч и перегонки культуральной жидкости из 100 кг опилок получают 15,6 л абсолютного спирта.EXAMPLE 4. The method is carried out according to Example 1, but sawdust is used as raw material, which is treated with an 11.0% sodium hydroxide solution at 80 ° C for 45 minutes. After culturing the yeast under anaerobic conditions for 52 hours and distilling the culture fluid from 100 kg of sawdust, 15.6 liters of absolute alcohol are obtained.

Пример 5. Способ осуществл ют согласно примеру 2, но куль-, тивирование ведут при концентрации кислорода 0,8 мг/л в течение 42 ч из 100 кг листьев сахарной свеклыExample 5. The method is carried out according to example 2, but the culture is carried out at an oxygen concentration of 0.8 mg / l for 42 hours from 100 kg of sugar beet leaves.

получают 12,0 кг сухих дрожжей и 2 л абсолютного спирта.get 12.0 kg of dry yeast and 2 liters of absolute alcohol.

Таким образом, предлагаемый способ переработки растительного сырь  позвол ет с помощью нового штамма икроорганизмов упростить процесс и сни зить энергозатраты за счет проведени  процесса предварительнойThus, the proposed method of processing plant raw materials allows using the new strain of microorganisms to simplify the process and reduce energy consumption by conducting a preliminary process.

31218927. 31218927.

обработки в более м гких услови х.ты различные виды растительного сыКроме того, переработке предпага-рь , в том числе промьшшенИые отхоемым способом могут быть подвергну-ды.processing in milder conditions. Various types of vegetable syAlso, the processing of presumptions, including industrial waste, can be subjected to the process.

Claims (4)

(57 )'1 .СПОСОБ ПЕРЕРАБОТКИ РАСТИТЕЛЬНОГО СЫРЬЯ ДЛЯ ПОЛУЧЕНИЯ БИОМАССЫ КОРМОВЫХ ДРОЖЖЕЙ И/ИЛИ ЭТИЛОВОГО СПИРТА, предусматривающий его предварительную обработку и последующее выращивание дрожжей, отличающийся тем, что, с целью упрощения способа, рас ширения сырьевой базы и уменьшения энергозатрат, предварительную обработку ведут путем воздействия на сырье при 80-100°C 0,5-10%-ным раствором кислоты или щелочи, а из дрожжей используют штамм Candida utilis № CU 2800199.(57) '1. METHOD FOR PROCESSING VEGETABLE RAW MATERIALS FOR PRODUCING FODDER YEAST BIOMASS AND / OR ETHYL ALCOHOL, providing for its preliminary processing and subsequent cultivation of yeast, characterized in that, in order to simplify the method, expand the raw material base and reduce energy lead by exposure to raw materials at 80-100 ° C with a 0.5-10% solution of acid or alkali, and from yeast use the strain Candida utilis No. CU 2800199. 2. Способ поп. 1, отличающийся тем, что для получения этилового спирта выращивание дрожжей ведут в анаэробных условиях.2. The method of pop. 1, characterized in that to obtain ethyl alcohol, yeast is grown under anaerobic conditions. 3. Способ по п. 1, отличающийся тем, что для получения биомассы, выращивание дрожжей ведут при концентрации кислорода3. The method according to p. 1, characterized in that to obtain biomass, the cultivation of yeast is carried out at an oxygen concentration 1,0-4,0 мг/л.1.0-4.0 mg / l. 4. Способ по π. 1, отличающийся тем, что для одновременного получения биомассы и этилового спирта, выращивание дрожжей . ведут при концентрации кислорода4. The method according to π. 1, characterized in that for the simultaneous production of biomass and ethyl alcohol, the cultivation of yeast. lead at an oxygen concentration 0,1-0,8 мг/л.0.1-0.8 mg / l. ,SU .„.1218927, SU. „. 1218927
SU813373695A 1980-12-23 1981-12-22 Method of processing vegetable raw material for producing biomass of fodder yeast and/or ethyl alcohol SU1218927A3 (en)

Applications Claiming Priority (1)

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HU803095A HU181771B (en) 1980-12-23 1980-12-23 Process for preparing fodder yeast and/or ethanol from plant wastes

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SU1218927A3 true SU1218927A3 (en) 1986-03-15

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CA (1) CA1178221A (en)
CS (1) CS241494B2 (en)
CU (1) CU21613A3 (en)
DD (1) DD201694A5 (en)
DE (1) DE3151176A1 (en)
FR (1) FR2496690A1 (en)
GB (1) GB2090514B (en)
HU (1) HU181771B (en)
PL (1) PL129919B1 (en)
SU (1) SU1218927A3 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
YU25697A (en) * 1997-06-18 1999-07-28 Dušan Ćirić Process of obtaining ethyl aclohol from cellulose
EP1865048A1 (en) 2006-06-09 2007-12-12 Cognis IP Management GmbH Process for the production of fatty acid alkyl esters by integrating fermentation and esterification
GB2530987B (en) * 2014-10-03 2017-06-21 Nafici Env Res (Ner) Ltd A method for processing straw

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3937845A (en) * 1975-01-08 1976-02-10 The United States Of America As Represented By The Secretary Of Agriculture Semi-solid fermentation of straw
ZA801703B (en) * 1979-07-02 1981-05-27 American Can Co Fermented acid hydrolyzates and fermentation process

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Авторское свидетельство СССР № 570642, кл. С 12 N 1/22, 1976. Авторское свидетельство СССР № 882511, кл. А 23 J 1/12, 1979. *

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FR2496690B1 (en) 1984-05-11
CS974881A2 (en) 1985-06-13
DE3151176A1 (en) 1982-09-02
PL234381A1 (en) 1982-08-16
DD201694A5 (en) 1983-08-03
FR2496690A1 (en) 1982-06-25
CU21613A3 (en) 1987-10-12
CS241494B2 (en) 1986-03-13
CA1178221A (en) 1984-11-20
GB2090514A (en) 1982-07-14
GB2090514B (en) 1984-04-11
PL129919B1 (en) 1984-06-30
HU181771B (en) 1983-11-28

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