SU1209100A1 - Method of making preparations of clarified and painted leaves - Google Patents

Description

The invention relates to botany and can be used to prepare serial preparations of leaf plates of various textures fB phylogenetic, systematic, 5 ecological and other studies that attract signs of leaf veining, as well as creating atlas of venation and morphotype collections.

The purpose of the invention is to preserve the veins of all orders and their configuration while maintaining the integrity of the sheet,.

Example 1. Fresh, fixed or dried leaves, for example, leaves of linden, birch, maple, bushwood and other leaves of a similar consistency — paper sheets are placed in 15% NaOH solution for 72 hours so that the leaves are completely immersed in 20 solution. Then the leaves are washed in running water for 1 hour and transferred to a solution of Javeel water prepared according to Furst: 1.20 g of bleach, 100 ml of distilled water, 1I, GBs of potassium carbonate, 100 ml of distilled water. Chlorine lime is stirred and allowed to stand in distilled water, then both solutions are mixed and left to stand for 4 days in a dark place, then filtered. The prepared lava water is stored in a dark place in a well-sealed bottle. Leaves are kept in a solution of lava water until the color completely disappears (1-2 hours). Bleaching is carried out in the light. After bleaching, the leaves are washed thoroughly with water and then with a 1% solution of acetic acid 40

25

Compiled by I. Marchenko. Editor L. Zaitseva Tehred Z. Kastelevich CorrectorM, Maksimishinets

Order 329/3 Circulation 679 Subscription

VNIIPI USSR State Committee

for inventions and discoveries 113035, -Moscow, Zh-35, Raushsk nab, d. 4/5

Filial PPP Patent, g, Uzhgorod, st. Project, 4

and again with water. To clear, the leaves are placed for 14 hours in a solution of chloral hydrate prepared according to Furst (S parts of chloral hydrate, 2 parts of distilled water), followed by washing with distilled water. To prepare for coloring, the leaves are dehydrated with 50% ethanol for 0.5 hours and transferred to an alcoholic 10% solution of safranin for 3 hours. The coloring time usually depends on the thoroughness of the whole previous treatment, in particular from washing with water and water. dye concentration. The color intensity is monitored by visual observation under a binocular loupe. For weak staining, a 20% safranin solution is used. Dyed leaves are placed in a 96% ethanol solution to differentiate. This procedure is repeated 2-3 times to remove excess dye. After differentiation, the leaves are placed for 3 days, in water, with daily replacements of stained water with fresh. Then the leaves are removed from the water and dried for 3 days. between the layers of nylon in filter paper under the press.

Example 2. Hard leaves, for example, some representatives of myrtle are treated similarly to example 1, but the material is placed in 30% NaOH solution for 120 h,

Example 3 In the case of very hard, leathery leaves, such as callophylum leaves, the treatment is carried out as in Example 1, but the material is placed in 30% NaOH solution at 60 ° C for 5 hours.

Claims (1)

_ METHOD FOR PREPARATION OF THE PRODUCTS OF LIGHTENED AND PAINTED LEAVES, including the processing of the starting material with alkali, decolorization with javelic acid, washing with acetic acid, staining with safranin and drying, characterized in that, in order to preserve the veins of all orders and their configuration in integrity as alkali, a 15-30% NaOH solution is used, after washing with acetic acid, the material is treated with chloral hydrate, then dehydrated with ethanol, after staining, differentiation is carried out with ethanol and maintained in water,