JPH06316874A - Dyeing of cotton - Google Patents

Abstract

PURPOSE:To dye a cotton fiber in various colors and color tones without using synthetic dye by treating a cotton fiber with an enzyme. CONSTITUTION:Cotton fiber such as raw cotton having greenish yellow color or brown color is immersed in a solution containing an oxidation-reduction enzyme such as peroxidase, polyphenol oxidase or bilirubin oxidase at room temperature to easily dye the fiber in various colors without causing the problems of the conventional dyeing process using synthetic dyes, e.g. problems of waste liquid treatment and industrial hygiene.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a cotton dyeing method using an enzyme.

[0002]

2. Description of the Related Art In the field of cotton dyeing, as an alternative to natural dyes, which are expensive and have a limited supply source, from the viewpoints of mass processing and dyeing cost reduction methods with the recent expansion of industrial production, Synthetic dyes are becoming popular. However, in the case of the dyeing method using a synthetic dye, not only a large amount of equipment and expense are required for waste liquid treatment, but also a synthetic dye which is not preferable in terms of work hygiene must be used in many cases.

As one of the solutions to such a problem, attempts have been made to develop colored raw cotton (colored cotton) by improving the varieties, but it is limited to cotton with a simple color such as green or brown. , Cotton of various varieties and shades has not been obtained.

[0004]

SUMMARY OF THE INVENTION In view of such circumstances in the art, the present invention is a simple method without the problems of waste liquid treatment and work hygiene as in the case of a dyeing method using a synthetic dye. Is to provide a cotton dyeing method capable of dyeing cotton in various colors and shades.

[0005]

That is, the present invention relates to a cotton dyeing method characterized by treating cotton with an enzyme.

In this specification, "cotton" includes raw cotton, cotton yarn, cotton cloth and various cotton products.

As enzymes for treating cotton, various enzymes derived from plants, animals or microorganisms can be used.
Particularly suitable enzymes are redox enzymes such as peroxidase, polyphenol oxidase, bilirubin oxidase, laccase, ascorbate oxidase,
Catalase and the like.

The enzyme is usually 0.001 in an aqueous medium, preferably a buffer having a pH of 4-9, eg a phosphate buffer.
100 mg / ml, preferably 0.1-10 mg / ml
It is used by dissolving it at the concentration of.

The cotton to be treated is dipped in the above enzyme-containing medium and usually treated at 4 ° C to 60 ° C, preferably 20 ° C to 50 ° C. The treatment time may be appropriately selected depending on the kind and concentration of the enzyme, the treatment temperature, the pH of the treatment bath, etc., but usually 1 to 24 hours can provide a sufficient dyeing effect.

In the above enzyme treatment, cotton dyeing is effectively promoted by coexisting with an enzyme substrate corresponding to the enzyme used, such as polyphenols, hydrogen peroxide, ascorbic acid, bilirubin or porphyrin derivative. be able to. Although the concentration of the substrate is not particularly limited, it is usually 0.001 to 100 mg / ml, preferably 0.1 to 10 mg / ml.

The enzyme-treated cotton is taken out of the treatment bath, thoroughly washed with water, and then dried (usually 20 ° C. to 60 ° C.) to give various colors and shades according to the treatment conditions. You get cotton. The above dyeing treatment does not impair the characteristics of cotton, such as texture. Further, the main dyeing raw materials used in the above dyeing treatment are substances that are usually present in nature, and the waste liquid treatment is extremely simple compared with the case of the dyeing method using a synthetic dye, and there is a problem in work hygiene There is almost no.

[0012]

EXAMPLES The present invention will be described below with reference to examples. Example 1 A defatted green-yellow thread was immersed in a solution prepared by dissolving 1 mg / ml (150 units / ml) of horseradish-derived peroxidase in a 0.1 M phosphate buffer (pH 7) and left at room temperature overnight. After that, it was taken out, washed with water, and dried to obtain a dark green treated cotton. Using a color difference meter (CR-200 manufactured by Minolta Camera Co., Ltd.), L value, a value and b value of green-yellow treated cotton and dark green treated cotton were measured, and the results are shown in Table 1. As is clear from Table 1, the L value, the a value, and the b value of the raw cotton were all decreased by the enzyme treatment, and this optical measurement result agrees with the visual result. The relationship between the color and tone and the L value, the a value, and the b value is as shown in FIG.

Example 2 Cucumber-derived ascorbate oxidase 1 mg / m
l (300 units / mg) and 50 mM of ascorbic acid were soaked in a phosphate buffer (pH 6.5) of brown raw cotton, left at room temperature overnight, taken out, washed with water, and dried to obtain a red color. A light brown treated cotton was obtained. Table 1 shows the measurement results of L value, a value and b value of the treated cotton and the treated cotton.

Example 3 A slightly brownish deep green treated cotton was obtained by performing the same treatment as in Example 1 except that bilirubin oxidase derived from a microorganism was used as the enzyme. Table 1 shows the measurement results of L value, a value and b value of the treated cotton and the treated cotton.

Example 4 A light yellow raw cotton was obtained by performing the same treatment as in Example 2 except that green yellow yarn raw cotton was used as the treated cotton. Table 1 shows the measurement results of L value, a value and b value of the treated cotton and the treated cotton.

Example 5 Example 3 except that brown yarn raw cotton is used as the treated cotton.
By performing the same treatment as above, dark brown treated cotton was obtained. Table 1 shows the measurement results of the L value, the a value, and the b value before and after the raw cotton treatment.

EXAMPLE 6 A light reddish brown cotton cloth was obtained by treating a cotton cloth produced from brown yarn raw cotton according to Example 2.

[0018]

[Table 1]

[0019]

According to the present invention, cotton can be treated in a variety of colors according to the treatment conditions by a simple method without the problems associated with waste liquid treatment and working hygiene as in the case of the dyeing method using a synthetic dye. And can be dyed in shades.

[Brief description of drawings]

FIG. 1 is a schematic diagram showing a relationship between color and tone and L value, a value and b value.

Claims (4)

[Claims] 1. A method for dyeing cotton, which comprises treating cotton with an enzyme. 2. The method according to claim 1, wherein the enzyme is a redox enzyme. 3. The method according to claim 1, wherein an enzyme substrate is allowed to coexist during the treatment. 4. A dyed cotton obtained by the method according to any one of claims 1 to 3.