SU118588A1 - Method for cultivating schizotanum crucium - Google Patents
Method for cultivating schizotanum cruciumInfo
- Publication number
- SU118588A1 SU118588A1 SU604799A SU604799A SU118588A1 SU 118588 A1 SU118588 A1 SU 118588A1 SU 604799 A SU604799 A SU 604799A SU 604799 A SU604799 A SU 604799A SU 118588 A1 SU118588 A1 SU 118588A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- cultivating
- schizotanum
- crucium
- proteolysis
- proteins
- Prior art date
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Description
. Изобретением вл етс способ культивировани трипанозом шизотрипаиум круци путем выращивани их на среде следующего состава; Д.Д. пептона200 мл М сной воды (0,5 кг м са на 1 л воды)200 мл Дистиллированной воды600 мл Сухих кров ных зерен20 г NasHPOi 12Н203 г КН2РО40,5 г Глюкозы10 г Тиамина (витамина BI)0,05 г Готовитс среда следующим образом. 1) Приготовление Д.Д. пептона. Свежие свиные желудки очищают и пленок и пропускают через м сорубку. На 1 кг фарща берут 0,5 л молодых пивных , 0,5 л дистиллированной воды и 40 мл химически чистой сол ной кислоты уд. в. 1,19. Протеолиз провод т в бутыл х . Бутыли встр хивают дл равномерного перемещивани смеси, став т в вод ную баню; смесь нагревают до 50° и держат при этой температуре до конца протеолиза белков. Через 1 час., а затем через 3 час. (от начала протеолиза белков при 50°) в каждую бутыль прибавл ют по 10 мл концентрированной сол ной кислоты дл поддержани оптимальной концентрации водородных ионов, котора постепенно падает по мере св зывани кислоты. В конце протеолиза белков, который продолжаетс при 50 от 5 до 7 час., остатки нерасщепленных белков превращаютс в однородную порошковидную массу, а гидролизат тер ет т гучесть и легко стекает со стекл нной палочки. Полна потер гидролизатом т гучести служит показателем дл прекращени протеолнза. Гидролизат отстаивают 2-3 дн , фильтруют через легкий ватный фильтр, закрывают ватно-марлевсй пробкой и оставл ют при 18-20° дл отстаивани и дальнейщего протеолиза высокомолекул рных белковых продуктов ферментами , перещедщими в фильтрат.. The invention is a method of cultivating trypanosis shizotripaium crucians by growing them on a medium of the following composition; D.D. peptone 200 ml of M of water (0.5 kg of meat per 1 l of water) 200 ml of distilled water 600 ml of dry blood grains 20 g NasHPOi 12H203 g KH2PO40.5 g Glucose 10 g Thiamine (vitamin BI) 0.05 g Prepare the medium as follows. 1) Cooking DD peptone. Fresh pork stomachs are cleaned and filmed and passed through a cutting board. For 1 kg of minced meat, take 0.5 l of young beer, 0.5 l of distilled water and 40 ml of chemically pure hydrochloric acid beats. at. 1.19. Proteolysis is carried out in bottles. The bottles were shaken to move the mixture evenly, placed in a water bath; the mixture is heated to 50 ° and kept at this temperature until the end of proteolysis of proteins. After 1 hour and then after 3 hours. (from the start of proteolysis of proteins at 50 °), 10 ml of concentrated hydrochloric acid is added to each bottle to maintain an optimal concentration of hydrogen ions, which gradually decreases as the acid binds. At the end of proteolysis of the proteins, which lasts at 50 for 5 to 7 hours, the remnants of the undigested proteins turn into a homogeneous powdery mass, and the hydrolyzate loses its thickening and easily drains from the glass rod. The total loss of hydrolyzate with gouging serves as an indicator for stopping the protease. The hydrolyzate is settled for 2-3 days, filtered through a light cotton filter, closed with a cotton-gauze stopper and left at 18-20 ° to settle and further proteolysis of high molecular weight protein products by enzymes transferred to the filtrate.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU604799A SU118588A1 (en) | 1958-07-26 | 1958-07-26 | Method for cultivating schizotanum crucium |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU604799A SU118588A1 (en) | 1958-07-26 | 1958-07-26 | Method for cultivating schizotanum crucium |
Publications (1)
Publication Number | Publication Date |
---|---|
SU118588A1 true SU118588A1 (en) | 1958-11-30 |
Family
ID=48390611
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU604799A SU118588A1 (en) | 1958-07-26 | 1958-07-26 | Method for cultivating schizotanum crucium |
Country Status (1)
Country | Link |
---|---|
SU (1) | SU118588A1 (en) |
-
1958
- 1958-07-26 SU SU604799A patent/SU118588A1/en active
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