SU130630A1 - Nutrient medium for tissue culture - Google Patents
Nutrient medium for tissue cultureInfo
- Publication number
- SU130630A1 SU130630A1 SU646318A SU646318A SU130630A1 SU 130630 A1 SU130630 A1 SU 130630A1 SU 646318 A SU646318 A SU 646318A SU 646318 A SU646318 A SU 646318A SU 130630 A1 SU130630 A1 SU 130630A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- nutrient medium
- tissue culture
- casein
- protein
- hydrolyzate
- Prior art date
Links
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
Дл культивировани тканей примен етс среда ЛЬ 199 (ПаркераК в состав которой, помимо солевых компонентов, вход т аминокислоты, витамины, основные колшоненты нуклеиновых кислот, источники липидов и другие вещества, всего в количестве 63 ингредиентов. Така среда вл етс дорогой и сложна в приготовлении. Известно применение в качестве источника аминокислот (при приготовлении питательных сред дл культивированн тканей) энзиматического гидролизата лактальбумииа или казеина. Однако ири использовании казеина приходитс добавл ть гидролизаты других белков, например, глютеина, или другие вещества (глютамин, цистеин и р д витаминов). Кроме того, дл получени в лабораторных услови х гидролизатов лактальбумина и казеина требуетс предварительное выделение белков и их очистка, а также использование больщнх количеств спирта, эфира, бидистиллированной воды и др. веществ.For the cultivation of tissues, an environment of L199 is used (ParkerK, which, in addition to salt components, includes amino acids, vitamins, basic nucleic acid sources, sources of lipids, and other substances, a total of 63 ingredients. Such an environment is expensive and difficult to prepare It is known to use the enzymatic hydrolyzate of lactalbium or casein as a source of amino acids (in the preparation of culture media for cultured tissues). However, when using casein, it is necessary to add hydro lysates of other proteins, e.g. glutein, or other substances (glutamine, cysteine, and a number of vitamins). In addition, in order to obtain in the laboratory hydrolysates of lactalbumin and casein, the preliminary isolation of proteins and their purification, as well as the use of alcohol , bidistilled water and other substances.
Эти затруднени устран ютс при изготовлении предлагаемой питательной среды путем использовани гидролизата белков обезжиренного молока (обрата) без выделени их в чистом виде.These difficulties are eliminated in the manufacture of the proposed nutrient medium by using skim milk protein hydrolyzate (skim milk) (skim milk) without isolating them in pure form.
Предлагаема питательна среда состоит из солевого раствора Э,ла , гидролизата белков обрата (0,1-0,15%) и сыворотки крови живочного . Сыворотку в количестве до 5% прибавл к-т в питательную среау перед ее употреблением.The proposed nutrient medium consists of saline E, la, hydrate of trabber protein (0.1-0.15%) and blood serum. Serum in the amount of up to 5% added to-t in a nutrient medium before its use.
Дл приготовлени гидролизата белков обрата к натуральному ибрату добавл ют 0,5 г трипсйпа и по 100 ЕД иа 1 мл обрата пенициллин; и стрептомицина. Смесь выдерживают в термостате при 37° в течение 20-24 час, довод т до кипени , фильтруют и высущивают.For the preparation of hydrolyzate of the protein of the shake, 0.5 g of trypsyp and 100 U of 1 ml of shabby penicillin are added to the natural ibrate; and streptomycin. The mixture is kept in a thermostat at 37 ° for 20-24 hours, brought to a boil, filtered and dried.
Гидролмзат белков обрата может примен тьс и в жидком виде.The hydrolm protein of the trabta can also be used in liquid form.
№ 130630- 2 Предмет изобретени No. 130630- 2 Subject of the invention
Питательна среда дл культивировани тканей, отличающа с тем, что в качестве источника аминного азота и других питательных веществ при приготовлении питательных сред, вместо гидролизата казеина и лактальбумина, нримен ют гидролизах белков обезжиренного молока (обрата), без выделени их в чистом виде.Nutrient medium for tissue culture, characterized in that, as a source of amino nitrogen and other nutrients, when preparing nutrient media, instead of casein and lactalbumin hydrolyzate, hydrolysis of skimmed milk proteins (skim milk) is used, without isolating them in its pure form.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU646318A SU130630A1 (en) | 1959-12-03 | 1959-12-03 | Nutrient medium for tissue culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU646318A SU130630A1 (en) | 1959-12-03 | 1959-12-03 | Nutrient medium for tissue culture |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU874278231A Addition SU1464146A2 (en) | 1987-07-06 | 1987-07-06 | Device for controlling gas pressure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU130630A1 true SU130630A1 (en) | 1960-11-30 |
Family
ID=48401743
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU646318A SU130630A1 (en) | 1959-12-03 | 1959-12-03 | Nutrient medium for tissue culture |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU130630A1 (en) |
-
1959
- 1959-12-03 SU SU646318A patent/SU130630A1/en active
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