SU1111054A1 - Device for fluid-proof sampling from machines operating in asceptic conditions - Google Patents

Abstract

1. DEVICE FOR SEALING SAMPLES FROM APPARATUS WORKING IN ASEPTIC CONDITIONS, containing sample collection, intermediate chamber, vacuum, pneumatic and hydraulic piping with valves and material piping, characterized in that, in order to improve device reliability and expansion, application. collection of samples installed with the possibility of vertical movement. and provided with an external mounted sleeve with an adapter, fitted with a valve in its upper part and with an overlapping membrane in the lower part and connected to an intermediate chamber, a physical pipeline located inside the intermediate chamber and provided with an outlet valve and a membrane blocking the inlet opening of the intermediate chamber, while the intermediate chamber, the adapter and the sleeve are mounted so that they can move relative to the material pipe of the pipeline. 2. The device according to claim 1, ex. (L of that, in order to visually check the tightness, the intermediate chamber is made transparent.

Description

ate

4 The invention relates to devices for hermetic sampling for the analysis of media from apparatus subjected to infection by foreign microorganisms, and can be used in microbiological, pharmaceutical and other sectors of the industry. A sampler is known that includes a cylindrical body and a stem mounted in a body so that it can be moved, the body is provided with a sealing element and the rod is provided with a sampler fij. The disadvantage of this device is the impossibility of sampling guaranteed aseptic conditions excluding the ingress of foreign microflora into the sterile cavities. The closest to the proposed technical essence and the achieved effect is a device for hermetic sampling, containing a collection of samples, an intermediate chamber, vacuum, pneumatic and hydraulic pipelines with valves, and material pipeline 2. However, the known device does not preclude asepsis disruption through the site connecting the receiving vessel to the intermediate chamber and the valves on the material piping and the receiving vessel. There is no possibility to check the sealing quality of these places. In addition, the device is unsuitable for sampling of solids, since, due to a repeated change in the direction of flow of the material upon sampling, the bulk of the particles of the product precipitate, not reaching the receiving vessel. The purpose of the invention is to increase the reliability and tightness of the device and to expand the scope of its application. The goal is achieved by the fact that in a device for hermetic sampling from devices operating under aseptic conditions, containing a collection of samples, an intermediate chamber, vacuum, pneumatic and hydraulic pipelines with valves and a material pipeline, the collection of samples is installed with the possibility of vertical movement and is equipped with mounted on the outside by its sleeve and adapter, fitted with a valve in its upper part and an overlapping membrane in the lower part and connected to an intermediate chamber, the material the pipe is located inside the intermediate chamber and is equipped with an outlet valve and a membrane blocking the inlet of the intermediate chamber, while the intermediate chamber, the adapter and the sleeve are installed with the possibility of moving relative to the material pipeline. The intermediate chamber is made of a transparent material. The drawing shows a device for hermetic sampling, a longitudinal section. The device contains a sample collector 1, which is installed with the possibility of axial movement with the help of a screw in the sleeve 3, an adapter 4, which through a flexible hose 5 and valve 6 is connected to the hydraulic pipe 7 of the supply of sterilizing solution and through the valve 8 to a vacuum pipe 9, using a clamp, for example, a bow, device 10 through the gasket 11 is connected to the intermediate chamber 12, which in its upper part through a flexible hose 13 and a valve 14 is connected to a vacuum pipe 9 and through a valve 15 to a pneumatic pipe water supply of sterile air 16 and inside which material pipe 17 is located. Adapter 4 has a valve 18 inside, for example, fluoroplastic to shut off the inlet of sample container 1 and a membrane 19 to shut off the entrance to the sleeve 3. In the intermediate chamber 12, valve 20 is installed, for example Fluoroplastic to shut off the outlet of the material pipe 17. Around the valves 18 and 20 there are through holes 21 for the passage of a sample of material. The material conduit 17 is connected to the intermediate chamber 12 through a membrane 22 for closing the upper opening of the intermediate chamber 12. A nut 23 is installed on the upper external threaded part of the material conduit 17, by means of which the intermediate chamber 12 together with adapter 4, sleeve 3 and sample container 1 has the ability axial movement — relative to the material pipe 17 as a result of moving it along the threads of the material pipe 17 and interacting through pins 24 with an annular groove 25 intermediate th camera 12. Intermediate chamber 12 is completed

from a transparent material, for example, from glass, for visual inspection of the quality of the sealing of valves and month of connections.

The device works as follows.

The pre-sterilized and sealed with valve 18 collection 1 of the sample together with the sleeve 3, screw 2, membrane 19 and adapter 4 with a clamping device 10 through the gasket 11 is connected to an intermediate chamber 12, which is sealed inside the material pipe 17 The intermediate chamber 12 is connected by a flexible hose 13 through a valve 14 to a vacuum pipe 9 and through a valve 15 to a pneumatic pipeline for supplying sterile air 16. Adapter 4 by a flexible hose 5 soy dinene through, valve 6 with hydraulic line 7 of the supply of sterilizing solution and through valve 8 with vacuum pipe 9. With open .6 and 14 and closed 8 and 15 valves, the entire space of the device with membrane and membranes 19 and 22 is filled with sterilizing solution. The valve 6 is closed and a vacuum is created above the surface of the solution. By the presence of air bubbles in the transparent chamber 12, rising from the gasket 11, or by their absence, the tightness of the connection between the adapter 4 and the intermediate chamber 12 is judged. After checking the tightness, the valve 14 is closed and a certain period of time is maintained for complete sterilization of the internal cavity. intermediate chamber 12. After this time has elapsed, valves 15 and 8 are opened, the sterilizing solution from intermediate chamber 12 and adapter 4 is removed through vacuum line 9 and This strip is flushed with sterile air. Then the valves 8 and 15 are closed. Using screw 2, sample container 1 moves down along the axis of sleeve 3 and moves away from valve 18, thereby opening the inlet of sample container 1. By briefly opening the valve 14, the internal cavity of the intermediate chamber 12, the adapter 4 and the collection 1 of the sample are evacuated, after which

help nut 23 intermediate chamber 12, adapter 4 and sleeve 3 with

the sample collector 1 is displaced downward relative to the material conduit-17, and the valve 20 opens its outlet. The product sample under the action of pressure drop between the apparatus and the sampler and under the action of gravity fills

collection of 1 sample. After sampling, the sample collector 1 with screw 2 presses against the valve 18 and its inlet orifice closes, and when the nut 23 rotates through the thread of the material pipe 17, the pins 24, slide along the groove 25, lift the device upwards and the valve 20 blocks the outlet of the material pipe 17 The sterilization cycle is repeated,

and at the same time the tightness of closing the material pipe 17 by valve 20 and sample container 1 by valve 18 is checked similarly to checking the tightness of the connection of adapter 4 and intermediate chamber 12. After the sterilization cycle and the tightness test are completed, collection of sample 1 together with sleeve 3, screw 2 and adapter 4 disconnect comes from

the intermediate chamber 12 and the flexible hose 5 and is transported to the place of analysis of the sample.

The application of the invention ensures the preservation of airtightness and the observance of aseptic conditions when sampling from sealed apparatus.

During preliminary tests, a prototype of the device was attached to a ZNRV-1D vacuum pump, a vessel with a sterilizing solution.

(hydrogen peroxide, alcohol), biological filter and sealing device. Tests were conducted using both a bulk (yeast) product and a liquid (hydrolysis

squirrel).,

Tests have shown that the proposed device provides for monitoring the tightness of the sampling point, to the apparatus and the reliability of closing the valves, the ability to take samples of not only liquid but also bulk products, as well as small losses of expensive product due to reduction of internal cavities connecting the apparatus with the sample collection.

Claims (2)

1. DEVICE FOR SEALING SAMPLING FROM APPARATUS WORKING IN ASEPTIC CONDITIONS, containing a sample collector, an intermediate chamber, vacuum, pneumatic and hydraulic pipelines with valves and a material pipeline, characterized in that, in order to increase the reliability of the device and expand its scope, the sampler is mounted with the possibility of vertical movement. and equipped with an external sleeve installed with an adapter provided with a valve in its upper part and an overlapping membrane in the lower part and connected to the intermediate chamber, the material pipe is located inside the intermediate chamber and is equipped with an outlet valve and an overlapping membrane of the inlet of the intermediate chamber, while the intermediate chamber, the adapter and sleeve are installed with the possibility of movement relative to the material pipeline. 2. The device according to π. 1, characterized in that, for the purpose of visual inspection of the tightness, the intermediate chamber is made transparent. 1111 (