SU1105125A3 - Installation for obtaining glycolipids - Google Patents

Abstract

1. An installation for producing glycolipids, containing a bioreactor for entering nutrient n growth substances, acid, alkali and oil and exhaust air, a separator for separating oil residues from biomass, a separator for separating the glycodipid solution from the cell mass, and that, in order to increase the efficiency of the installation by more complete separation of glycolipids, it is equipped to separate glycolipids from the cellular mass of an additional H1 3 m bioreactor with a heat sink and pipelines for sche.yuchi, salt and water, wherein the input is connected to the bioreactor vkodom separator for separating oil from biomass ostatkep and vkod bioreactor sosdi1-; f -1 to the input separator DL otdelen1 glikolipshdov solution of Cellular ;; masses. .

Description

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io ;; poTi-Hiie O; uijci: h i to the defaulted iipOMbiiiiju-D; О1 ::. ;:. private gi to h :: a11ovkam d, dd 11C). WEDDING and quality; quality and quality; adding water to water 5 pings: tae - oi n ldst d ;;;;

you are CHHD1eN I NefTg.

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 D i DCH OJi DD ,, .. S. 1: 1; W-PD1 t D) D GOG;

dd dgdd dt: uge; dn hell and growth

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ddra.ootad; dl (J nszdukha, ;; 3/1, DNT OTD, D-dl SDS OSSDG -

ccw heditti d i. : g: g dptor dd stgudedepi

: -.; ... .

,; ddd.dd cd ;; .-: D ::; : hdZotadg -; with / Odddtdk; Effdutidd and;; - ;;: D.LIOJDif.;:: J where.lodlod liks h1: 1: lov,

 icMf; dzg) bsme11d 1 - {op-s; seds dffekdD1Dg-D1 vci dt {o} kp iivic M bt; her odud - l; d {and gd; .d: and: l dd.)

Ukdzddd de. GD reached; gc order

that: -DG; DCHKD DJD; KHULDe1D I 1DD1KOLD iTHJiOH (i dar) adt), co.i, yerzhaid bd-) act) udd dddda gdggatstsudddum and rotoFihix thing, sour;; u; ; Naf td and BbiHoyia will work with DU Du Du; and a separator for separation from biomass: a residue of defty, a DUY sedanor to sample the glycidal solution from the common mass, providing for the removal of gidolipids from the mass of the body mass, to get rid of the gdolipid from the mass of the cell, to get the gel from the bed, to get the gdolipid solution from the mass of the dyed mass from the common mass; truboprozod, am1-1 udd feed; ; tseudochd 5 uow and vod ;, we will arrive at the entrance of the bioreactor from the separator d sy to the separator dd of separation from the biomass of petroleum residues and the output of the breeder is connected to the entrance of the separator

separation of solution-gltgkidididov from cellular mass.

Settlement for addition of 1dididid (d vaidan) Ullspakgorom dd from paci opa gd kooddpidov from the check mass, come the entrance, the extractor is connected to the exit C: iarator - dd separation from the biomass of oil residues.

ia of FIG. 1 shows an installation for the production of GJD lipolipids (I variant); Ng1 fig, 2 - the same, L option.

The set-up (Fig. 1) contains a bioreactor 1 for the input of nutrients and cereal substances, ACID, oil generosity and output of the spent body, separator 2 dd of separation from biomas, oil residue, separator 3 of the glycolipids solution from the mass, additional bioreactor 4 with heat exchanger 5 and pipelines 6 and 7 for supplying P1e: e:) chi, water and water, mixer 8 with stirrer 9, pipelines 10-13 for the oil node, water; and air, obog; with oxygen, separator 1 for the water-drift mixture coming from well 15 to pipeline 16, pipelines 17-28 and injection well 29.

The stop works as follows.

 wells 15 to the separator G4 posgudae -; - water oil to the mixture for exhaust, dp5 defty from water. Oil and oil; and the wood pipelines 13 and 10 are supplied to the b1 jureactor 1. Through the pipeline 17, weft oil from the well does not provide enough water for dewatering. Through a pipeline, 18, take air from the bioreactor 1 and exhaust it, which; n the bottom biorector 4.

From bioreactor 1, in which microorganisms are grown, heterogeneous c, mes, 1 is directed through conduit 10 to cer, fa, pa, Top 2, in which unspent oil is separated. This oil is either directly withdrawn from the system via line 11, or is directed through line 17 to dewatering. A bioreactor 4 is connected to the separator 2, in which its temperature, pH and osmotic pressure are drastically changed to enrich the culture suspension with glycolipids. . In bioreactor 4, equipped with heat exchanger 5, air is introduced through pipes 18. In addition, alkali is introduced into bioreactor 4 through pipeline 6 and water from the field or brine is introduced through pipeline 7. The unused air exits the conduit 18. Via the withdrawal conduit 19, the oil-free culture suspension is withdrawn from biorector 4 to separator 3, in which the cell mass is separated from the glycolipids of the aqueous phase. The cell mass introduced from the separator 3 through the tubes of the wire 20 can be partially or fully returned via the pipeline 21 to the bioreactor 1 as a carbon source. The glycolipid-containing aqueous phase is directed through conduit 22 in a mixture of a body 8 with a stirrer 9, into which a dispersing agent is fed through conduit 23 - or a dissolving agent. Here the mass is stabilized by vigorous stirring. Stabilization of the mass can be accomplished by sonication. With the help of tap pipe 24 and tap pipes 2 and 26, it is possible to establish the desired ratio between glycolipid dispersion and heated water into the reservoir, which is fed via pipelines 27 and i to the injection well 29. The installation (Fig. 2) contains a bioreactor 1 for input nutrient and growth substances, acids, alkalis and oil and exhaust air, separator 2 for separating residual oil from biomass, separator for water-oil mixture 3 coming from well 4, pipeline 6, directing mass to extractor 7, pipelines 8- 11, evaporate There are 12, pipelines 13 and 14, a mixer 15 with a stirrer 16, a pipe 17 and an injection well 18. A bioreactor 1 is connected to the separator 3 for supplying the water-oil mixture, from which the heterogeneous mixture is directed to the separator 2. The aqueous phase and the residual oil come from the separator 3 through the pipeline 5 for the dehydration of oil, while the cell mass through the pipeline 6 is sent to the extractor 7, into which the extractant is fed through the pipeline 8. The extracted cell mass is removed from the extractor via line 9. It can be partially or fully returned to bioreactor 1 via line 10. The glycolipid-rich extractant is sent via line 11 to the evaporator 12. The extractant distilled therein by line 13 is returned to the extractor, and the glycolipids are returned to The discharge pipe 14 is directed to the mixer 15, in which they are mixed and dispersed in the flooding water supplied through the pipe 17 in the presence of a solvent agent. The rest of the apparatus in their positional numbers and purpose correspond to the apparatus of the apparatus shown in FIG. one . The advantage of the proposed unit for the complete separation of the glycolipids obtained in the aerated bioreactor is that the biosynthesis in it is carried out in two stages, and in the first stage cellular tissue mass is obtained in optimal conditions and as a result of glycolipids. and in the second stage, glycolipids are separated from the cellular mass by abrupt changes in temperature, pH, osmotic pressure, or extraction. With the help of the proposed installation, it was possible for the first time to achieve the almost complete isolation of the obtained glycolipids and obtain an enriched culture solution with a concentration of more than 600 mg / l. The proposed installation allows us to use the resulting glycolipids efficiently and effectively, in particular in the form of a stable dispersion. The installation also has the advantage that the cellular mass as a carbon source and for using the nutrients contained in it can be partially or completely returned to the process, and field water can be used to obtain an aqueous solution in the bioreactor.

51 K; 5 1 2 56

In addition, the proposed field and oil users can be located near the plant- (| n-h as the source of the well mix for flooding of petroleum - alkanes for its development.