SU1100306A1 - Culture medium for culturing entomopathogenic bacteria - Google Patents

Abstract

NUTRITIONAL ENVIRONMENT FOR CULTIVATION OF ENTOMOPATHOGENIC BACTERIA, including maize flour As a carbon source, sources of nitrogen and mineral salts and tap water, characterized in that, in order to increase the yield of bacteria, as well as cheaper nutrient medium, it contains as a source of nitrogen and mineral salts, squeezing liquid - flax processing waste With the following ratio of components, may.%: Maize flour 0.5-1 Squeezing liquid processing waste 3-4 (by dry weight) Tap water Remaining

Description

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about l

The invention relates to the microbiological industry - in particular to nutrient media for growing microorganisms producing bacterial preparations.

Known nutrient medium, predstavlivaya a cleaning fluid obtained during the processing of flax solo G1 3.

However, this nutrient medium is not suitable for the growth of entomopathogenic bacteria, since it contains mainly organic molecular acids (up to 4000-500-0 mg / l) as a carbon source. Closest to the proposed technical essence and the achieved effect is a nutrient medium for growing entomopathogenic bacteria 2, including corn flour as a source of carbon, sources of nitrogen and mineral salts and water. The medium as a source of nitrogen and mineral substances contains activated sludge (in dry. Or liquid form) in the following ratio of components:

Maize flour, 10-15 g Dry activated sludge, g 10-30 Water tap, ml Up to 1000 or. corn flour, 10-15 g of liquid activated sludge, ml 250-750 Water tap water, ml Up to 1000 Dry (liquid) activated sludge obtained from feed yeast plants, in its nutritional properties, has valuable properties: contains 50% crude protein, vitamins and mineral elements. Nutrient media containing activated sludge from feed plants are prepared as follows. The required amount of dry sludge or corn flour (or other products or by-products containing polysaccharides) is weighed and poured into the reactor, according to the calculation, tap water with a temperature of 30 40 ° C, stirred, set the pH to 7.8-8.0 using a 30% aqueous solution of caustic alkali. The mixture is boiled for 15 minutes, stirred, and the pH value, which should be 7.5-8.0, is checked. The resulting medium is sterilized at or passed through a continuous sterilization unit.

After sterilization, the pH of the medium should be in the range of 6.8-7.2. The resulting fermentation medium for the production of biomass is seeded with a liquid seed culture (inoculum) in an amount of 3-4% relative to the fermentation medium.

However, the known nutrient medium contains paraffins 1.9–2.9%, toxic and wild-smelling products formed during acid hydrolysis of wood, and nitrogen dominates mainly in the form of microbial biomass and is difficult to extract. bacteria. In addition, the environment is quite expensive.

O The aim of the invention is to increase the yield of bacteria, as well as reduce the cost of the nutrient medium.

This goal is achieved by the fact that the nutrient medium for the growth of entomopathogenic bacteria, including corn flour 6 as a carbon source, nitrogen sources and mineral salts and water, contains squeezing waste from flax processing in the following ratio of components, by weight, as a source of nitrogen and mineral salts. %:

Cornmeal 0.5-1.0

Squeeze liquid waste recycling

flax 3-4 (by dry weight)

Piped water Else

The essence of the invention is

next.

Wringing liquid contains (dry matter) in the following ratio of components, wt.%:

Crude Protein 20-40

Mineral residue

(of which 30-40%) 8-20.0 Pectic substances 2-3 Lignin 10-12

Cellulose14-25

Polysaccharides3-4

Squeezing of soaked flax is obtained under production conditions from the 1st pair of rollers of the squeeze-washing machine, which creates the pressure on the soaked flax-salt (trust) within 40-80 kg / cm across the width of the layer. The flax extract obtained under the high pressure of the rollers contains protein and pectin substances from flax stalks that do not pass freely into the washing liquid during biological lotion. When the fabric washers and the cells of swollen flax straw pass through the rollers of the squeeze-out machine, their contents are transferred to the squeezing liquid, which can serve as a microorganism in the composition. ve nutrient medium source of nitrogen and carbon.

The amount of squeezing liquid in a nutrient medium is regulated by the interests of the production of preparations on this nutrient medium. It is convenient to express this value in terms of the amount of dry matter (in a liquid or dried squeeze fluid). With a 1-2% squeezing liquid in the nutrient medium (dry matter) in the culture liquid, the increase in the titer of microbial bodies does not exceed 1.5-2.5 billion / ml, which is not enough. With increasing squeezing liquid concentration up to 6-7% (on dry

Indicators

IL BVK dry 3 May.% Corn flour 1 wt.%

Tap water up to 1000 ml

2.67 + 0.06 / 3.43 + 0.06

Note. Indicators of accumulation of microorganisms when growing nts proposed medium and on the medium, taken as a prototype; in the numerator for Bacillus thuringiensis var galleriae Jsak; in denominator, for Bacillus thuringiensic var dendro-r limus.

In addition, as studies have shown, this nutrient medium with

to the substance) and the process of vegetative cell division is slowed down, as soon as the sporulation process proceeds, the formation time of free spores lengthens up to 4-5 days and twisted. . A comparison of the proposed nutrient medium with the known (prototype) is presented in table. one.

Table 1

Proposed Wednesday

Prototype

Sludge precipitate

dry 3 May.%

Corn flour 0.5 wt.%

Piped water Up to 100%

3.01 + 0.18 / 3.26 ± 0.16 6.55 / 6.95 6.70 / 6.80

7,

7.40 / 7.80

microorganisms grown on it are characterized by high insecticides, are also better eaten by insects and caterpillars, which leads to their higher death (for example,

caterpillars of vegetable moth (galleria mellanella Z).

In tab. Figure 2 shows data showing the effect of the nutrient medium on the insecticidal nature of bacteria.

Table 2

2 million

46.7 200 thousand

ae 22.2

2 million

42.2

Vair dendrolimus

200 thousand

31.1 Example Weighed and poured into the cooking apparatus dried from

82.2

97.8 77.8 100.0

57,8

88/7

51.1

53.3

88.9 press liquid in the amount of 3% and corn flour; in the same container, according to the calculation, tap water d with a temperature of 30-40 ° C is poured, mixed, adjusted to pH 7.8-8.2 using 30% sodium hydroxide solution. The mixture is boiled for 15-20 minutes, stirred, the pH is checked, the value of which should be about 7.4-7.8. The resulting medium is sterilized at 121-128 s or is passed through a continuous sterilization unit. After sterilization, the pH of the medium should be in the range of 6.6-7.2. The obtained medium for fermentation for the purpose of biomass production is seeded with a liquid seed culture (inoculum) in an amount of 4-5% relative to the fermentation medium. A culture of Bacillus thuringiensis var galleriae and var dendrolimus is used.

The results of determining the effectiveness of the squeezing liquid in comparison with the medium of the active or production of protein-vitamin concentrate (BVK) are given in Table. 3

Table 3

Claims (1)

Nutrient medium for the cultivation of entomopathogenic bacteria, including corn flour as a carbon source, sources of nitrogen and mineral salts, and tap water, characterized in that, in order to increase the yield of bacteria, as well as reduce the cost of the nutrient medium, it contains as a source of nitrogen and mineral salts squeezed, g liquid - waste of flax processing. In the following ratio of components, May.%: Corn flour 0.5-1 Extraction liquid flax processing waste 3-4 (for sou Tap water home weight) The rest m