SU1039483A1 - Method of diagnostics of latent necrotic complications of patients ill with traumatic injuries - Google Patents

Abstract

METHOD OF DIAGNOSIS OF LATENT NECROTIC complications in patients with traumatic lesions by determining the optical density in the biochemical study of blood, characterized in that, in order to accelerate the process of blood isolated acid-soluble fraction was determined by its absorbance at wavelengths of 260 and 320 nm. calculate the difference in density and by increasing this indicator relative to the norm diagnose a latent necrotic complication. (L

Description

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SA: The invention relates to medicine, namely to methods for diagnosing tissue necrosis. A known method for the diagnosis of latent necrotic processes in patients with traumatic injuries by means of a biochemical blood test 1. However, this method is not sufficient and specific for the diagnosis of necrotic processes in injuries. Closest to the proposed technical essence is a method for the diagnosis of latent necrotic processes in patients with traumatic injuries by means of a biochemical blood test. Serum is obtained from the analyzed blood, it is added to a sleepy broth in which hay bacillus is grown, and the optical density of broth 2 is examined during the cultivation of a hay bacterium. However, this method is lengthy, it is about 24 hours. The aim of the invention is to accelerate the method. The goal is achieved according to the method of diagnosing latent necrotic complications in patients with traumatic injuries by determining the optical density in a biochemical examination of blood, the acid-soluble fraction is separated from the blood, its optical density is determined at wavelengths of 260 and 320 nm, the difference is calculated density and to increase this indicator relative to the norm diagnose latent necrotic complications. The method is carried out as follows. Whole blood taken from a patient with a volume of 0.25 ml was diluted in 10 ml of a 5% perchloric acid solution (the ratio of the volumes of blood and perchloric acid was 1:40). After five minutes of incubation at room temperature, the suspension is centrifuged at 300 rpm for 5 minutes. The supernatant is discharged and the optical density is measured at a wavelength of 260 nm (Onjgo) on a spectrophotometer, and then at a wavelength of 320 nm (OGD), the difference between these parameters is then calculated, and by increasing this difference compared to the norm damage. Normally, the difference is 9 ± 2. Example 1. Patient N. P., 43 years old, was admitted to the clinic 20 minutes after receiving a work injury. On admission, a diagnosis was made: traumatic amputation of the first and third toes of the left foot. After Three hours after the patient was injured, an operation was performed. The patient studied the difference in optical densities (OP2bO-e2o / ml) .. the blood at admission was 16, and in the state of necrosis - 26. After the wound healed, the difference indicator 01 Tuyo.; 2o; almost normal values. Thus, the increase in the difference between OPGDA-2 (in the postoperative period indicated necrosis in the area of the schv. Example 2. Patient V.F., 59 years old, was admitted to the clinic 4 hours after receiving a household injury. On admission, the diagnosis was left colo-wound thighs. After 5 hours after the patient was injured, the patient underwent primary surgical treatment of the wound. The patient examined the blood on admission. On the second day, the difference in the OP raft fishery rate was 48, then after 7 days of active treatment of the wound and when the patient was discharged home (13 June), when the wound healed, the difference in OD was 22 and 14 / ml on the fifth day after the operation indicated necro: soft tissues. Example 3. Sick K.I., 54 years old, was admitted to the clinic 1.5 hours after falling on the street. fracture of both ankles of the right tibia with dislocation of the foot outward. 3.5 hours after injury, the patient was operated on. The patient's blood was examined, the difference of Ovabo-Zao / i-was 26, after the removal of sutures - 20, which indicated the presence of an open necrotic process. After 1 month Ill re-addressed in connection with pain. The study showed that OPZb-mgo / mV difference left 45, which indicated necrosis of soft tissues. After removing the plaster cast, a wound was found on the inner side of the ankle joint in the area of the former suture. Started appropriate treatment. The fact of wound injury at normal body temperature and blood and urine tests, and in the absence of pain, is indicative of irradiation, necrosis and suppuration. After re-hospitalization and wound healing, a blood test and determination of Onjgj, .. j22y indicator turned out to be 14 units, which confirmed the approach to a normal state. The method allows you to get a quick response to the sun; -the growth of the beKpo HyecKoro process and the accuracy of the result. The use of the invention provides a 34

The possibility of early diagnosis of necrotic-performing analysis eliminates any

process and allows us to predict complications in patients. Way

The complication of traumatic pains is simple and possible for common and thus ensure timely use in any medical-effective treatment facility.

1039483

Claims (1)

METHOD FOR DIAGNOSIS OF HIDDEN NECROTIC COMPLICATIONS IN PATIENTS WITH TRAUMATIC DAMAGES by determining the optical density in a biochemical blood test, characterized in that, in order to accelerate the method, an acid-soluble fraction is isolated from the blood, and its optical density is determined at wavelengths of 260 and 320 nm. calculate the difference in densities and by increasing this indicator relative to the norm, a latent necrotic complication is diagnosed.