SU1034358A1 - Strain of rhizobium bacteria of soe rhizobium japonicum 634b for use in preparing bacterial composition of nitragin for soe - Google Patents

Abstract

Strain of soybean nodule bacteria Rhizobium japonicum 6346 (collection of the All-Union Scientific Research Institute of Agricultural Microbiology, used to make the bacterial preparation nitragin under soybeans.

Description

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The invention relates to the field of microbiological agents for increasing the yield of legumes and represents a new strain of soybean nodule bacteria Rhlzobium jaronicum 6346, intended for the industrial manufacture of the preparation of nodule bacteria natragin under soybean.

An effective agrotechnical method for increasing the yield of soybean is the presowing treatment of its seed or highly active breeding strains of nodule bacteria in the form of various forms of nitragin tl 1

A known strain of soybean nodule bacteria Rh, japonicum 646, on which soybean nitragin 23 is made.

However, it has low aero-fixing activity and weak competitiveness.

The aim of the invention is to obtain a new strain of soybean nodule bacteria, surpassing the known strain in terms of nitrogen-fixing and competitive ability.

To achieve this goal, a strain of soybean nodule bacteria {Ihizoblum japonicum 6346 collection of the All-Union Research Institute of Agricultural Microbiology) is used to make the bacterial preparation nitragin for soybeans.

An example. Bacteria 6346 divisions) from soybean nodules of the variety Kolkhida 4, which grew on meadow soils of Western Georgia, Grow on nutrient media of the following composition: Lupine agar, g / l: O., 5; KHjPO 0.5; MgS04 0.2; NaCl 0.2; CaSO 0.1; (NH4) 2Mo04 traces; mannitol 20.0; lupine flour 10.0; agar 20.0 pH of the medium 6.8-7.0. Pea agar, g / l: pea 100.0, sucrose 20.0, agar 20.0 pH 6.8-7.0.

Morphological and cultural signs.

The size of the cells-W-daily culture on lupine agar 2,0-3,0x1,01, 2 microns. The sticks, at a young age, are mobile, monotrichi. Bacteroids have the form of larger, slender sticks. Gramotr: Slow growing culture. Stroke on lupine agar is abundant mucous white shiny. Colonies on pea agar on day 7-8 are small, up to 1 mm round, convex white mucous membranes. Physiological and biochemical characteristics:

Milk with litmus is not peptonized, poorly alkalinized. Gelatin is not diluted. Aerobes, optimum temperature 26-28 ° C. Attitude to carbohydrates. Use many carbohydrates with alkalinization of the environment. Galactose, rhamnose, maltose, sucrose, mannitol, dextrin, lactose are actively absorbed, weaker than glucose and very weakly xylose. Do not absorb fiber and starch. Relation to nitrogen sources. Grows well on media with sodium nitrate, ammonium salts, and tyrosine. On MPA do not grow. Signs of a resistant strain 1. The strain is not pathogenic. Strain 6346 is stored on lupine agar and transplanted once every 6 months. It is stored in the collection of nodule bacteria of the All-Union Scientific Research Institute of Agricultural Microbiology and has a registration number of 6346.

The nitrogen-fixing activity of strain 6346 was studied in vegetation experiments and determined from the height of the crop and the accumulation of protein in it. In the same experiments, the competitive ability of strain 6346 was determined by the percentage of nodules formed by it during mixed inoculation with strain 646. The results of the experiments are presented in the table.

Nitrogen-fixing activity and competitive ability of strains of soy nodule bacteria 646 and 6346

As can be seen from the table, strain 6346 actively fixes atmospheric nitrogen in symbiosis with soybean, as a result of which the yield of ash mass of plants, bacteria-induced strains of this, was 54.1% higher than during the inoculation | 1 with a sample-type of 646. using strain 6346 oastenn were not only more powerful, but also had a more intense green okoasku because the symbiotic nitrogen from the air flowed into them more intensely than in plants bacterized with strain 646. Protein accumulation by plants, bacterized with strain 6346, more than2 happy - higher than in the embodiment with shtanmom prototype. Bacteria of strain 6346 form the overwhelming majority of root nodules (85%) with mixed inoculation with strain 646, the competitive ability of strain 6346 is higher than in the prototype strain,

Simultaneously with an increase in yield, the use of strain 6346 improves grain quality due to the more intense accumulation of protein in it, the content of which in the soybean grain, the bacterized strain 6346, is 1.1% higher than during bacterization by the prototype strain. one,

The pgamma 6346 bacteria have good technological properties, which allows it to be used for the industrial production of the bacterial preparation of nitragin for soybeans.

By the decision of the Interdepartmental Commission of the Geographical Network of Experiments with Nitragin from February 10, 1981, the strain Rh. Japanonic 6346 is recommended for use in the industrial manufacture of nitragin for soybeans.

Claims (1)

A STRAW OF STRAWBERRY SOY BACTERIA А DAROSHIM 634B USED TO PRODUCE A NITRAGINE BACTERIAL PRODUCT UNDER SOY (57) A strain of nodule bacteria of soybeans of Koboobygish daropgsish 6346 of an agricultural scientific preparation. 1034358