CN1928068B - Lilaceous streptomycete and application thereof - Google Patents
Lilaceous streptomycete and application thereof Download PDFInfo
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- CN1928068B CN1928068B CN2005100295193A CN200510029519A CN1928068B CN 1928068 B CN1928068 B CN 1928068B CN 2005100295193 A CN2005100295193 A CN 2005100295193A CN 200510029519 A CN200510029519 A CN 200510029519A CN 1928068 B CN1928068 B CN 1928068B
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Abstract
The present invention provides one kind of lilac Streptomyces lavendulae strain SPRI-94166 in the preservation number of CGMCC No. 1368. The strain may be fermentation cultured to obtain culture, which may be used in preventing and controlling various kinds of weeds, such as pigweed, cockspur grass, goose grass, nut grass, moleplant, etc.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of new Lilaceous streptomycete SPRI-94166 and application.
Background technology
Because domestic geographical environment, the weather condition of China vary, Microbial resources are considerably abundant.People seek new microbial strains by the region at various different latitude, height above sea level and weather.Usually, morphological specificity, cultural characteristic and the physiological and biochemical property etc. by bacterial strain also usually adopt the sequence of the 16srDNA that detects microbial strains to come the clearly classification position of this bacterial classification to the microbial strains discriminating of classifying simultaneously.
By cultivating, in the meta-bolites that obtains, seek various activeconstituentss, and and then research and develop into various medicines (comprising medicine, agricultural chemicals, veterinary drug etc.) gathering the microbial strains get throughout the country.This technology is widely used, and as medical antibiotic penicillin, Streptomycin sulphate etc., the Avrmectin etc. of jingganmycin, the multiple acarid of control and the noctuid of the rice sheath blight disease of preventing and treating is arranged aspect agricultural, for animals.
Summary of the invention
Purpose of the present invention just is to provide a kind of Lilaceous streptomycete SPRI-94166.
Another purpose of the present invention is to provide the application of described Lilaceous streptomycete SPRI-94166.
A kind of Lilaceous streptomycete SPRI-94166 of the present invention belongs to lilac grey streptomycete (Streptomyces Lavendulae), and on May 16th, 2005 in Chinese common micro-organisms preservation center preservation, preserving number is CGMCC No.1368.
Lilaceous streptomycete SPRI-94166 of the present invention gets for the contriver separates from the soil that gather in China Guangxi in June, 1994.The sequence of the morphological specificity of this bacterial strain, cultural characteristic, physiological and biochemical property and 16SrDNA and bacterium classification result are as follows:
1, morphological specificity: synthesize an agar at Gao Shi with the inserted sheet method, cultivate the form of observing mycelia and spore respectively with opticmicroscope and electron microscope after 10～15 days on the glucose asparagine agar, the result shows, this bacterium substrate mycelium does not have tabula, does not rupture, no conidium, aerial mycelium branch head, and it is luxuriant to grow, fibrillae of spores is longer, there are 2-5 ring spiral, spore ellipse, smooth surface in the top.
2, physiological and biochemical property and utilization of carbon source see Table 1:
The physiological and biochemical property of table 1, SPRI-94166 bacterial strain and utilization of carbon source
| Utilization of carbon source | The result | Physiological and biochemical property | The result |
| D-glucose | + | Gelatine liquefication | + |
| The D-wood sugar | + | Milk solidifies | - |
| D-N.F,USP MANNITOL | + | Milk peptonizes | + |
| D-fructose | + | The starch hydrolysis | - |
| L-arabinose | + | Grow on the Mierocrystalline cellulose | - |
| The L-inositol | - | Nitrate reduction | + |
| The L-rhamnosyl | - | H2S produces | + |
| Silk floss sugar | - | Class melanochrome produces | + |
| Sucrose | + | Tyrosine oxidase produces | + |
3, cell walls is formed: 94166 bacterial strains contain LL-DAP (LL-DIAMINOPIMELIC ACID diaminopimelic acid), glycine, atypism sugar, and the cell walls chemical composition belongs to the I type.
4, the 16srDNA sequence of 94166 bacterial strains of the present invention is shown in SEQ ID NO:1.
According to form, physiological and biochemical property and 16srDNA sequential analysis, this bacterial strain belongs to the lilac grey monoid (Lavendulae) of streptomyces (Streptomyces), so be decided to be lilac grey streptomycete (Streptomyces Lavendulae).
Lilac grey streptomycete of the present invention, its cultural method both can be deep-layer liquid and had cultivated, and also can be circle and cultivated.In order to help payable cultivation, the spore suspension of above-mentioned production bacterium should be inoculated on the substratum, the gas mixing that works of going forward side by side is mixed.
Nutrition source in the substratum be there is no special regulation, can make and contain carbon source, nitrogenous source and other nutrition sources that is usually used in microorganism culturing in the substratum.Wherein carbon source can be starch, dextrin, glucose, glycerine, sucrose, N.F,USP MANNITOL etc., and nitrogenous source can be meat peptone, soybean cake powder, peanut powder, yeast, meat extract, rice bran, corn steep liquor, wheat skin, ammonium salt, urea, nitrate and other organic or inorganic nitrogenous compounds.Other nutrition sources can suitably add some inorganic salts, for example metal-salts such as salt, phosphoric acid salt and potassium, calcium, manganese, iron.Can add in case of necessity that some move, plant, the mineral wet goods is as defoamer.
Culture condition such as temperature, PH, time be there is no strict restriction, be as the criterion with the production that is suitable for producing bacterium, and to select the highest condition of active constituent output for well.For example, the PH scope of substratum can be 6-8, with near neutral for well; Culture temperature is at 20-35 ℃, and air flow is with (ventilation ratio: refer to that per minute passes through the volume of air of nutrient solution and the ratio of nutrient solution volume) between the ventilation ratio 1: 0.3 to 1 to 4.The component of these substratum, PH, culture temperature, aeration condition etc. all should carry out suitable adjustment according to practical situation, to obtain best effect.
Lilac grey streptomycete of the present invention is cultivated by fermentation, and the culture that obtains can be used for preventing and kill off multiple farmland weed, specifically, can prevent and kill off various weeds such as Eclipta prostrata, Amaranthus retroflexus, lamb's-quarters, barnyard grass grass, Herba Eleusines Indicae, Rhizoma Cyperi, Semen Euphorbiae, nutgrass flatsedge.
Description of drawings
Fig. 1 is the stereoscan photograph of the strain growth form of Lilaceous streptomycete SPRI-94166 (Streptomyces Lavendulae) CGMCC No.1368.
Embodiment
Below by specific embodiment, further specify the present invention.
Lilaceous streptomycete SPRI-94166 of the present invention submits Chinese common micro-organisms preservation center preservation on May 16th, 2005, and preserving number is CGMCC No.1368.
The starting material that below relate to all can obtain by commercially available, and below the concentration that relates to be weight concentration.
The cultivation of embodiment 1, Lilaceous streptomycete SPRI-94166
Lilaceous streptomycete SPRI-94166 Streptomyces caviscabies CGMCC No.1369 is synthesized an agar with the inserted sheet method at Gao Shi, on the glucose asparagine agar, in 28 ℃ of incubators, cultivated 10～15 days, take out the form of observing mycelia and spore respectively with opticmicroscope and electron microscope, the electron microscope scanning result as shown in Figure 1.
As seen from Figure 1, the bacterium substrate mycelium of Lilaceous streptomycete SPRI-94166 of the present invention does not have tabula, does not rupture, no conidium, aerial mycelium branch head, and it is luxuriant to grow, and fibrillae of spores is longer, and there are 2-5 ring spiral, spore ellipse, smooth surface in the top.
The fermentation of embodiment 2, Lilaceous streptomycete SPRI-94166
Ratio with glucose 3%, starch 5%, groundnut meal 1%, soybean cake powder 2%, peptone 0.1%, yeast extract paste 0.4%, sodium-chlor 0.2%, sal epsom 0.5%, ammonium nitrate 0.2%, dipotassium hydrogen phosphate 0.05% is made substratum, PH is adjusted to 7.2, sterilization.Lilac grey streptomycete (Streptomyces Lavendulae) the SPRI-94166 CGMCC No.1368 bacterial classification inoculation that grows in the Gao Shi inclined-plane in this substratum, was cultivated 36-40 hour down at 28 ℃; With this culture is seed, is inoculated in the fermentation culture that above-mentioned substratum forms with the ratio of 5-20%, and 28 ℃ of aeration-agitations were cultivated 90-96 hour.
The application of embodiment 3, Lilaceous streptomycete SPRI-94166
In 20ml water, add the 1-2g agar powder, be heated to 60-100 ℃, agar powder is fully dissolved.Add embodiment 2 while hot and cultivate the fermented liquid 1ml that obtains, shake up, respectively the seeds of weeds such as Eclipta prostrata, Amaranthus retroflexus, lamb's-quarters, barnyard grass grass, Herba Eleusines Indicae, Rhizoma Cyperi, Semen Euphorbiae, nutgrass flatsedge is sprinkling upon the surface of solidifying agar after to be cooled the solidifying, simultaneously prepare eight groups of agar that do not add fermented liquid as stated above, and be sprinkled into above-mentioned eight kinds of weed seeds respectively as blank.Cultivated 7 days at 28 ℃, observe weed seed and germinate and growing state, the results are shown in Table 2.
The fermented liquid of table 2, Lilaceous streptomycete SPRI-94166 is to the Plant diseases test-results
Result according to embodiment 3 shows, Lilaceous streptomycete SPRI-94166 of the present invention (StreptomycesLavendulae) CGMCC No.1368 cultivates by fermentation, contain herbicidal active compounds in its culture, can be used for preventing and kill off multiple farmland weed, specifically, can prevent and kill off various weeds such as Eclipta prostrata, Amaranthus retroflexus, lamb's-quarters, barnyard grass grass, Herba Eleusines Indicae, Rhizoma Cyperi, Semen Euphorbiae, nutgrass flatsedge.Can further be developed as a kind of novel agricultural herbicide.
Sequence table
＜110〉Shanghai Pesticide Research Institute
＜120〉a kind of Lilaceous streptomycete and application thereof
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<160>1
<170>PatentIn?version?3.3
<210>1
<211>1481
<212>DNA
<213>Streptomyces?lavendulae
<220>
<221>16srDNA
<222>(1)..(1481)
<400>1
gacgaacgct?ggcggcgtgc?ttaacacatg?caagtcgaac?gatgaaccgg?cttcggtcgg 60
ggattagtgg?cgaacgggtg?agtaacacgt?gggcaatctg?cccttcactc?tgggacaagc 120
cctggaaacg?gggtctaata?ccggatatga?cacggggtcg?catgatctcc?gtgtggaaag 180
ctccggcggt?gaaggatgag?cccgcggcct?atcagcttgt?tggtgaggta?gtggctcacc 240
aaggcgacga?cgggtagccg?gcctgagagg?gcgaccggcc?acactgggac?tgagacacgg 300
cccagactcc?tacgggaggc?agcagtgggg?aatattgcac?aatgggcgaa?agcctgatgc 360
agcgacgccg?cgtgagggat?gacggccttc?gggttgtaaa?cctctttcag?cagggaagaa 420
gcgagagtga?cggtacctgc?agaagaagcg?ccggctaact?acgtgccagc?agccgcggta 480
atacgtaggg?cgcgagcgtt?gtccggaatt?attgggcgta?aagagctcgt?aggcggcttg 540
tcacgtcgga?tgtgaaagcc?cggggcttaa?ccccgggtct?gcattcgata?cgggcaggct 600
agagttcggt?aggggagatc?ggaattcctg?gtgtagcggt?gaaatgcgca?gatatcagga 660
ggaacaccgg?tggcgaaggc?ggatctctgg?gccgatactg?acgctgagga?gcgaaagcgt 720
ggggagcgaa?caggattaga?taccctggta?gtccacgccg?taaacgttgg?gaactaggtg 780
tgggcgacat?tccacgtcgt?ccgtgccgca?gctaacgcat?taagttcccc?gcctggggag 840
tacggccgca?aggctaaaac?tcaaaggaat?tgacgggggc?ccgcacaagc?agcggagcat 900
gtggcttaat?tcgacgcaac?gcgaagaacc?ttaccaaggc?ttgacataca?ccggaaagca 960
ttagagatag?tgcccccctt?gtggtcggtg?tacaggtggt?gcatggctgt?cgtcagctcg 1020
tgtcgtgaga?tgttgggtta?agtcccgcaa?cgagcgcaac?ccttgttctg?tgttgccagc 1080
atgcctttcg?gggtgatggg?gactcacagg?agactgccgg?ggtcaactcg?gaggaaggtg 1140
gggacgacgt?caagtcatca?tgccccttat?gtcttgggct?gcacacgtgc?tacaatggcc 1200
ggtacaatga?gctgcgatgc?cgcgaggtgg?agcgaatctc?aaaaagccgg?tctcagttcg 1260
gattggggtc?tgcaactcga?ccccatgaag?tcggagttgc?tagtaatcgc?agatcagcat 1320
tgctgcggtg?aatacgttcc?cgggccttgt?acacaccgcc?cgtcacgtca?cgaaagtcgg 1380
taacacccga?agccggtggc?ccaacccctt?gtgggaggga?atcgtcgaag?gtgggactgg 1440
cgattgggac?gaagtcgtaa?caaggtagcc?gtaccggaag?g 1481
Claims (4)
1. a Lilaceous streptomycete SPRI-94166 (Streptomyces Lavendulae) is characterized in that, this microorganism classification ownership is the lilac grey streptomyces, and its preserving number is CGMCC No.1368.
2. the application of the described Lilaceous streptomycete SPRI-94166 of claim 1 is characterized in that, is used to produce the weedicide of preventing and kill off following weeds: Eclipta prostrata, Amaranthus retroflexus, lamb's-quarters, barnyard grass grass, Herba Eleusines Indicae, Rhizoma Cyperi, Semen Euphorbiae, nutgrass flatsedge.
3. application as claimed in claim 2 is characterized in that, described Lilaceous streptomycete SPRI-94166 produces weedicide by fermentation culture.
4. application as claimed in claim 3 is characterized in that, the fermentation culture conditions of described Lilaceous streptomycete SPRI-94166 is:
Cultivated 90-96 hour 28 ℃ of following aeration-agitations; Substratum is formed wt%: glucose 3%, starch 5%, groundnut meal 1%, soybean cake powder 2%, peptone 0.1%, yeast extract paste 0.4%, sodium-chlor 0.2%, sal epsom 0.5%, ammonium nitrate 0.2%, dipotassium hydrogen phosphate 0.05%.
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| CN2005100295193A CN1928068B (en) | 2005-09-08 | 2005-09-08 | Lilaceous streptomycete and application thereof |
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| CN2005100295193A CN1928068B (en) | 2005-09-08 | 2005-09-08 | Lilaceous streptomycete and application thereof |
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| CN1928068A CN1928068A (en) | 2007-03-14 |
| CN1928068B true CN1928068B (en) | 2011-03-09 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5694708B2 (en) * | 2009-08-18 | 2015-04-01 | 三井化学アグロ株式会社 | A-87774 compounds or salts thereof, processes for producing them, and agricultural chemicals containing them as active ingredients |
| CN103540556B (en) * | 2013-11-12 | 2015-04-15 | 青岛明月蓝海生物科技有限公司 | Streptomyces lavendulae and application of Streptomyces lavendulae to preparation of algae microbial fertilizer |
| CN105941485A (en) * | 2016-05-14 | 2016-09-21 | 尤馨雅 | Efficient garden herbicide |
| CN106962405A (en) * | 2017-03-27 | 2017-07-21 | 陕西博秦生物工程有限公司 | A kind of application of close rotation streptomycete in parasitics weeds are prevented and kill off |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1486613A (en) * | 2003-08-14 | 2004-04-07 | 南京农业大学 | Batch production process of biological herbicide |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1486613A (en) * | 2003-08-14 | 2004-04-07 | 南京农业大学 | Batch production process of biological herbicide |
Non-Patent Citations (5)
| Title |
|---|
| 周世宁.番茄内生链霉菌S5的分离及其除草活性.农业生物技术学报13 4.2005,13(4),全文. |
| 曹理想 |
| 谭红铭 |
| 邱志琦 |
| 邱志琦;曹理想;谭红铭;周世宁.番茄内生链霉菌S5的分离及其除草活性.农业生物技术学报13 4.2005,13(4),全文. * |
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Granted publication date: 20110309 Termination date: 20170908 |