RU1824384C - Strain of rhizobium leguminosarum bv viceae bacteria for producing bacterial fertilizer for pea - Google Patents

Abstract

Usage: microbiological industry, production of means for increasing the yield of legumes and relates to a new strain of nodule bacteria for preparing nitragin for peas. SUMMARY OF THE INVENTION: Rhlzobium legumlnosarum bv strain. vlceae was isolated from the nodules of pea plants grown on sod-podzolic soil. It was deposited in the VNIISKhM collection under No. 1070. The fertilizer obtained on the basis of this strain allows increasing the pea yield by 13.5-17%. 3 tab.

Description

The invention relates to microbiological means for increasing the yield of legumes and represents a new strain of pea nodule bacteria Rhizobium leguminosarum bv.viceae 1070 for the preparation of nitragin (rhizotorfin) for peas.

The aim of the invention is to obtain a strain of pea nodule bacteria having the ability, in comparison with the reference strain 250a, not only to provide a more significant increase in yield, but also to improve its quality by increasing the protein content in the grain and green mass of peas.

The inventive strain 1070 was isolated from the nodules of pea plants of the Ramonsky cultivar, which grew in the Borodno region of the Kiev region of the sod-podzolic soil.

Strain 1070 at number 31 is stored in the collection of the Institute of Microbiology and Virology of the Academy of Sciences of the Ukrainian SSR (Kiev), This number 1070 was assigned to it in 1988 after the donation of the All-Union Collection of Nodule Bacteria of the All-Russian Research Institute of Agricultural Microbiology (Leningrad). Identified by the Bergi Brief Identifier.

Morphological and cultural characteristics.

The strain Rhizobium leguminosarum bv.viceae 1070 grows well at a temperature of 26-28 ° C in a liquid and solid medium of the following composition, g / l: beans (peas) 100; sucrose 20; NaCl 1.0; agar 15.0 pH 6.8-7.0. In a liquid medium, the culture is grown on shakers at 220 rpm.

When inoculated on bean agar, growth is plentiful, slimy. The size of the colonies of two diurnal cultures does not exceed 5 mm. Milk-colored colonies with a shiny surface and even edges. At a later age, the colonies mucus and spread. Culture is fast growing, not

with

to

4

with

00

4

sporiferous, gram-negative. The cell size is 3.5 x 0.6 μm, the shape is rod-shaped. The nature of the flagellum is perigrihial.

The claimed strain grows well on mannitol-yeast agar of the following composition, g / l: sodium phosphate disubstituted 0.5; magnesium sulfate 0.4; sodium chloride 0.1; chalk 0.1; mannitol 10.0; yeast extract 1.0, pH 7.0-7.2. Colonies of two-day cultures are superficial, convex, white with even edges. Diameter 2-4 mm. The cell size is 2.5x0.5 μM. Mucus is weak. When withdrawn from the medium, the colonies do not tighten.

Strain 1070 grows weakly on Ashby's nitrogen-free solid medium. When sowing with a stroke on the third day, separate dense, small, round, 1-2 mm diameter brilliant colonies of milky white color grow. In the process of growth, on the 5-7th day, their size slightly increases. Colonies go nuts behind the loop.

Physiological and biochemical properties.

Aerobe. The optimal pH value of 6.8-7.2. On MPA and MPB, culture does not grow. It does not dilute gelatin. Litmus milk alkalizes, forming a serum zone in the form of a ring.

Attitude to carbohydrates. It absorbs glucose, lactose, mannose, maltose, fructose, galactose and mannitol. Slightly weaker glycerin and sorbitol with the formation of acid. On a Norris liquid medium, when grown on a rocking chair, acidification occurs to pH 5.0. Starch does not absorb.

The relation to nitrogen sources was established on the regulatory (factory) environment of the following composition,%: molasses 2.0; Corn extract 1.0; monosubstituted potassium phosphate 0.025; potassium phosphate bisubstituted 0,025; magnesium phosphate 0.02; chalk 0.20; pH 6.9-7.2; ammonium sulfate 0.1.

In successive series of experiments, ammonium sulfate was replaced by ammonium nitrate, potassium nitrate, sodium nitrate, and calcium nitrate. The growth pattern indicates that the claimed strain assimilates these compounds. Slowly restores nitrates.

Strain 1070 has no special marker features. Non-pathogenic and non-toxic. Replanted once every 6 months. It is stored on bean agar in a refrigerator at 4-6 ° C.

Strain 1070 is able to tolerate sodium sulfate and sodium chloride salinization in the range from 0.1 to 3%. which creates the prerequisites for its use on saline soils. In addition, the inventive strain tolerates conditions of insufficient moisture, which indicates its high environmental adaptability.

The effectiveness of strain 1070 was verified in vegetation and field experiments. The data on the effect of the strain on the pea crop of the Uladovskiy jubilee and Albatros varieties under the conditions of the vegetation experiment are presented in Table. 1. As can be seen from the above data, the claimed strain

has a higher nitrogen-fixing activity than the prototype strain. This property has a positive effect on pea yield and protein content. In the variants using the claimed strain 1070 for inoculation with the correspondingly taken varieties, it was 16.5 and 13.9% larger than when using the reference strain.

In the growing experiments with pea varieties Streletsky and Malinovka, the claimed strain also showed higher efficiency compared to the reference strain (Table 2).

Rhizobium strain test

legumlnosarum bv.viceae 1070 in field experiments was carried out over a number of years. Data on the effect of presowing inoculation of pea seeds of the Uladovskiy jubilee variety on yield and quality of green mass

are presented in table. 3.

Thus, field trials confirmed the high efficiency of the claimed strain. Compared with the prototype strain, he increased the yield

green pea mass by 28 and 40 kg / ha and contributed to an increase in the collection of crude protein by 7.3 and 27.9 kg / ha, respectively.

Claims (1)

The claims Bacterial strain Rhizobium leguminosarum bv.viceae VNIISM 1070 for the preparation of fertilizer for peas. i a 6 liters 1 table 2 Table 3