SU1017302A1 - Method of simulating hearing acute loss - Google Patents

Description

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This invention relates to experimental physiology and otoneurology.

There is a known method for simulating acute hearing loss by making a joint on the neck of an animal, dissecting the muscles of the neck, exposing the bull to its lower i wall, removing the chunks of Promontorium, destroying the cochlea, coagulating the proximal ends of the auditory and vestibular nerves 1.

There is also known a method of modeling acute hearing loss by a midline incision in the neck of an animal with a mid / intact incision at the level of the dorsal bone, a platysma incision, penetration deep into the special d. Djgastrlci, pulling down the subsopharyngeal nerve and the carotid artery. lower wall removal of promohtorium, destruction of the cochlea under the control of L2J vision.

However, the known methods are traumatic, accompanied by damage to the vestibular apparatus, dysfunction of the internal organs and, consequently, high animal mortality.

The aim of the invention is the isolated shutdown of the auditory analyzer, the reduction of the morbidity of the operation and the prevention of vestibulopathies.

The goal is achieved by the fact that it is safe to simulate acute hearing loss by accessing the cochlea and destroying it, making a skin incision around the base of the auricle, extending the frame to the zygomatic process, exfoliating the eardrum along the perimeter, removing the system of auditory ossicles and strainer the stirrup, the outer wall of the inert maze is thinned, and when the snail is destroyed, the integrity of the webbed labyrinth is preserved.

The method is carried out as follows.

The animal is placed on a small operating table with a head holder attached to it. The position of the operated animal is on the abdomen with limbs fixed to the table.

Anesthesia - Nembutal 0 mg per I kg weight. The position of the head is natural. Mount the head holders for the upper and lower jaws. The hair on the dorsal part of the skull between the basics of the auricums is clipped. A skin incision is made along the line around the base of the cisternal shell, starting from the front fold and ending at the back-bottom. The auricle is pulled downwards. Two clips are inserted into the skin wound, and bluntly, the skin is separated from the underlying tissues to the ear canal in a blunt way.

 In the lower pole of the wound, a cartilage-like protrusion covering the eardrum is isolated. By moving it away, the eardrum is circumferentially separated by 3 / length so that its connection is maintained only in the lower part of the bone cola (a. The high muscle above the zygomatic process is blunt and shifted with a spatula to the top. the cavity is expanded with boron. The opening of the auditory ossicles and the stirrup tensioner are removed.

Under the microscope, the bony wall of the cochlea is thinned with a thin boron to the membranous labyrinth. Bone chips are removed by suction directly at the moment of bone thinning. Remnants of the Maze bone wall are removed. Without destroying the integrity of the membranous labyrinth, crushing the needle with the blunt end of a cut needle destroys the scroll.

Next, they conduct a revision of the tympanic cavity, draining it and impose a skin suture. The duration of the operation is 20-30 minutes.

Example. The cat in the state of anesthesia is placed on the operating table. The position is on the abdomen with limbs fixed to the table, the head is fixed in the natural position by the holder to the upper and lower cheekliuses. Wool between the bases of the auricles is sheared.

A skin incision is made along the line enveloping the base of the auricle, starting from the anterior crease and ending at the back-bottom. The auricle is pulled down. Two clips are inserted into the skin wound, in a blunt way downward, the skin is separated from Subject Tissues to the ear canal. In the lower pole of the frame, xy and 1 shaped protrusion is isolated, covering the eardrum. By moving it away, the eardrum is separated along the perimeter by 3 / lengths so that its connection is maintained only in the lower part of the bone ring. The temporal muscle above the zygomatic process is separated by a blunt path and shifted upwards with a scapula. The bone ring of the entrance to the tympanic cavity is expanded with boron. The opening of the auditory ossicles, and the stirrup tensioner are removed. The stirrup pad remains in place. The cavity is dried. The following landmarks are visible: the round window, the promontory — the bed of the cochlea, the surrounding elevations — the outer walls of the vestibule and the semicircular canals. The bony wall of the cochlea under an operating microscope, ifphonchial thin boron to the membranous labyrinth. Bone chips are removed by suction directly at the time of bone thinning. After removal of the bone wall residues, the labyrinth opens the greyish-colored wall of the membranous labyrinth. Not violating its integrity, the destruction of the cochlea is performed by pressurizing the blunt end of the cut needle. The phenomena of dysfunction of the vestibular analyzer in case of the destruction of the cochlea without marking the integrity of the membranous labyrinth are not marked, i.e. a model is created with a complete acute hearing loss without interfering with the functioning of the closely connected to the auditory vestibular analyzer. After the operation, some of the animals could be taken for experiments the next day, the rest - on the 2nd - 3rd. After the destruction of the cochlea, the auditory cavity is inspected and dried. Impose a skin iv. The duration of the operation on one side. 20-30 min. According to the proposed method, 25 operations were performed. Cases of death of animals as a result of injuries incompatible with life are not noted. The proposed method makes it possible to isolate the auditory analyzer in isolation, to prevent the occurrence of vestibulopathies, to prevent damage to the great vessels and nerve trunks of the neck, and related disorders of the internal organs.

Claims (1)

METHOD FOR SIMULATING ACUTE MUTION OF HEARING by accessing and destroying the cochlea, in order to isolate the auditory analyzer off, reduce trauma and prevent vestibulopathy, a skin incision is made that goes around the base of the auricle, expand the wound to the zygomatic process, flake the eardrum around the perimeter, remove the auditory ossicle system and tension stapes, thin the outer wall of the bone labyrinth, and with the destruction of the cochlea maintain the integrity of the membranous about the maze. Μ co to> I 017302