SU1008656A1 - Octatione determination method - Google Patents

Abstract

METHOD FOR DETERMINING OCTATION by treating the sample with chemical reagents, about tl and. In order to increase the specificity of the method, the sample is dissolved in ethanol, a buffer solution is added at a pH of 6.2-6.5, then a solution of brss 1tmolovogo blue is added and the appearance of blue staining of the reaction mixture The presence of octation is determined.

Description

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ate

Yes

The invention relates to the analysis of organic compounds, relates to the determination of medical preparations and can be applied in the work of control and analytical laboratories of chemical and pharmaceutical plants and pharmacy departments.

A known method for determining octation by treating a sample with chemical reagents.

However, the known method flies around with low sensitivity and is not specific.

The purpose of the invention is to increase the specificity of the method.

The goal is achieved by the fact that according to the method of determining the octation by treating the projections with chemical reagents, the sample is dissolved in ethanol, the buffer solution is added at a pH of 6.2-6.5, then a solution of bromoimol blue is added and the blue color of the reaction mixture is added the presence of octation is determined.

The determination of octation is carried out at pH 6.2 (acetate buffer solution). The octation with a reagent under these conditions forms a blue-colored compound ((v c3tKc 600–610 nm). The reagent solution (without octathione) has a yellow-green color (420,430 nm). A good response to octation with bromthymol blue is high-contrast, as in the formation of an octathion associate with a reagent, the bathochromic shift of the maximum of the absorption spectrum by 170 nm with respect to the absorption spectrum of the reagent occurs.

The method for determining octation is specific, as other medicinal substances derived from quaternary ammonium bases (prozerin, benzamone, dicoline, dimecolin, naphtamon, metacin, benzohexonium, pentamine, diplacin, ditilin, oxazil, qualified, quaterone, cotarnine, mercury, quarnone, cotarnine, mercury, quarnone, cotarnine, diphroline, dithilin, oxazil, qualified, quaterone, cotarnine, quartone, cotarnine, diphroline, dithiline, oxazil, qualified, quaterone, cotarnine, diphroline, dimekolin, nimethanol octation determination. These data indicate a high specificity of the qualitative reaction to octation.

The sensitivity of the method 20 / MKC / ml. The method does not require hard-to-reach reagents, expensive devices, express (a blue color appears immediately after the addition of the reagent).

Example. 5 mg of pharmacopoeial preparation (octation) are dissolved in 5 ml of ethanol and diluted with distilled water to 25 ml. In a 5 ml graduated graduated sludge take 1 ml of the resulting solution, add 0.1 ml of a solution of bromothymol blue (concentration 10 mol / l), 0.5 ml of acetate buffer solution (pH 6.2) and distilled water to a volume of 5 ml. In the presence of octation in the test drug, the solution becomes blue. The control solution (without octation) is colored yellow-green.

Claims (1)

METHOD FOR DETERMINING OCTATION by processing the sample with chemical reagents, about l and. h and u. " and with the fact that, in order to increase the specificity of the method, the sample is dissolved in ethanol, a buffer solution is added at a pH of 6.2-6.5, then a solution of bromothymol blue is added, and the presence of a blue color in the reaction mixture determines the presence of octathion.