SG11202110853SA - Systems, methods and compositions for recombinant in vitro transcription and translation utilizing thermophilic proteins - Google Patents

Systems, methods and compositions for recombinant in vitro transcription and translation utilizing thermophilic proteins

Info

Publication number
SG11202110853SA
SG11202110853SA SG11202110853SA SG11202110853SA SG11202110853SA SG 11202110853S A SG11202110853S A SG 11202110853SA SG 11202110853S A SG11202110853S A SG 11202110853SA SG 11202110853S A SG11202110853S A SG 11202110853SA SG 11202110853S A SG11202110853S A SG 11202110853SA
Authority
SG
Singapore
Prior art keywords
recombinant
compositions
systems
methods
vitro transcription
Prior art date
Application number
SG11202110853SA
Other languages
English (en)
Inventor
Michael Humbert
Alexander Koglin
Charlie Villanueva
Original Assignee
Ntxbio Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ntxbio Llc filed Critical Ntxbio Llc
Publication of SG11202110853SA publication Critical patent/SG11202110853SA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1014—Hydroxymethyl-, formyl-transferases (2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/02—Hydroxymethyl-, formyl- and related transferases (2.1.2)
    • C12Y201/02009—Methionyl-tRNA formyltransferase (2.1.2.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04001—Polyphosphate kinase (2.7.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07048—RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
SG11202110853SA 2019-04-12 2020-04-13 Systems, methods and compositions for recombinant in vitro transcription and translation utilizing thermophilic proteins SG11202110853SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962833555P 2019-04-12 2019-04-12
PCT/US2020/028005 WO2020210833A1 (en) 2019-04-12 2020-04-13 Systems, methods and compositions for recombinant in vitro transcription and translation utilizing thermophilic proteins

Publications (1)

Publication Number Publication Date
SG11202110853SA true SG11202110853SA (en) 2021-10-28

Family

ID=72751486

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202110853SA SG11202110853SA (en) 2019-04-12 2020-04-13 Systems, methods and compositions for recombinant in vitro transcription and translation utilizing thermophilic proteins

Country Status (11)

Country Link
US (1) US20220275028A1 (zh)
EP (1) EP3953366A4 (zh)
JP (1) JP2022535651A (zh)
KR (1) KR20210151928A (zh)
CN (1) CN114269767A (zh)
AU (1) AU2020273197A1 (zh)
BR (1) BR112021020322A2 (zh)
CA (1) CA3136639A1 (zh)
IL (1) IL287152A (zh)
SG (1) SG11202110853SA (zh)
WO (1) WO2020210833A1 (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113025548B (zh) * 2021-04-08 2023-06-20 西南大学 基于kosakonia sp.菌株生产2’-岩藻糖基乳糖的重组菌及其方法和应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010019888A (ko) * 1999-08-31 2001-03-15 복성해 써머스 칼도필러스 ｇｋ24로부터 분리한 코스미드 클론 3의 유전자 염기서열 및 아미노산서열
EP1279736A1 (en) * 2001-07-27 2003-01-29 Université de Nantes Methods of RNA and protein synthesis
WO2003038117A2 (en) * 2001-10-30 2003-05-08 The Board Of Trustees Of The Leland Stanford Junior University Enhanced in vitro nucleic acid synthesis using nucleoside monophosphates
JP4790843B2 (ja) * 2007-03-30 2011-10-12 富士通株式会社 能動消音装置および能動消音方法
JP5948673B2 (ja) * 2011-03-10 2016-07-06 ジーンフロンティア株式会社 リポ多糖混入量を低減した蛋白質合成用組成物、該組成物を用いた蛋白質製造方法
WO2016044328A1 (en) * 2014-09-18 2016-03-24 The Regents Of The University Of California Single-molecule phenotype analysis
ES2921017T3 (es) * 2016-12-30 2022-08-16 Natures Toolbox Inc Sistema de expresión libre de células que tiene una nueva regeneración de energía basada en polifosfato inorgánico

Also Published As

Publication number Publication date
AU2020273197A1 (en) 2021-10-28
CN114269767A (zh) 2022-04-01
BR112021020322A2 (pt) 2021-12-14
EP3953366A1 (en) 2022-02-16
WO2020210833A1 (en) 2020-10-15
US20220275028A1 (en) 2022-09-01
JP2022535651A (ja) 2022-08-10
CA3136639A1 (en) 2020-10-15
EP3953366A4 (en) 2023-07-19
KR20210151928A (ko) 2021-12-14
IL287152A (en) 2021-12-01

Similar Documents

Publication Publication Date Title
IL276715A (en) Preparations and methods for administration of membrane protein
EP3586852B8 (en) Modified cell expansion and uses thereof
EP3864152A4 (en) METHODS AND COMPOSITIONS FOR EDITING RNAs
EP3956449A4 (en) METHODS AND COMPOSITIONS FOR EDITING RNAs
EP3635943A4 (en) SYSTEM AND METHOD FOR ASYNCHRONOUS MULTIMODE MESSAGE TRANSMISSION
MX2017012206A (es) Proliferacion ex vivo de celulas epiteliales.
EP3802812A4 (en) ANTI-RNA TARGETED FUSION PROTEIN COMPOSITIONS AND METHODS OF USE THEREOF
EP3908294A4 (en) MODIFIED CELL EXPANSION AND USES THEREOF
EP3551212A4 (en) NEW RECOMBINATED KLOTHO PROTEINS AND COMPOSITIONS AND METHODS INCLUDING THEM
EP3833751A4 (en) COMPOSITIONS AND METHODS OF GENOME MODIFYING WITH CAS12A PROTEINS
IL286776A (en) Preparations and methods for preparing t cell preparations and their uses
EP3911355A4 (en) RECOMBINANT LUBRICINS, COMPOSITIONS AND METHODS OF USING THE SAME
IL291284A (en) Systems and methods for expression of proteins
EP3880785A4 (en) SLIME SYSTEMS FOR IN VITRO CELL CULTURE
EP3573627A4 (en) SYSTEMS AND METHODS OF HEMATOPOIETIC CELL EXPANSION USING HYDROGELS
EP3886877A4 (en) METHODS FOR SIMULTANEOUS EXPANSION OF SEVERAL IMMUNE CELL TYPES, RELATED COMPOSITIONS AND USES SAME IN CANCER IMMUNOTHERAPY
IL287152A (en) A method and mixture system for recombinant transcription and translation is not safe in the use of thermophilic proteins
EP3858981A4 (en) METHOD OF EXPANSION OF HEPATOCYTES IN VITRO AND APPLICATION
EP3411482A4 (en) ANTIBODIES AND ELISA KITS FOR THE ASSAY OF ALPHA KLOTHO PROTEIN
EP4013768A4 (en) COMPOSITIONS AND METHODS FOR HIGHLY EFFICIENT PROTEIN PRECIPITATION
EP3841194A4 (en) IN VITRO FERTILIZATION SYSTEM AND ASSOCIATED COMPONENTS THEREOF
EP3665188A4 (en) IMPROVED IN VITRO TRANSLATION / TRANSLATION SYSTEM (TXTL) AND ITS USES
EP3766356A4 (en) MULTI-PH BUFFER FORMULATION AND COMPOSITION TO FACILITATE PROTEIN DIGESTION, AND USE OF THEM
IL305817A (en) Systems and methods for protein expression
PT3358967T (pt) Formulações para a redução de emissões bovinas