SG11202108524SA - Methods for scarless introduction of targeted modifications into targeting vectors - Google Patents
Methods for scarless introduction of targeted modifications into targeting vectorsInfo
- Publication number
- SG11202108524SA SG11202108524SA SG11202108524SA SG11202108524SA SG11202108524SA SG 11202108524S A SG11202108524S A SG 11202108524SA SG 11202108524S A SG11202108524S A SG 11202108524SA SG 11202108524S A SG11202108524S A SG 11202108524SA SG 11202108524S A SG11202108524S A SG 11202108524SA
- Authority
- SG
- Singapore
- Prior art keywords
- scarless
- introduction
- methods
- targeting vectors
- targeted modifications
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/106—Plasmid DNA for vertebrates
- C12N2800/107—Plasmid DNA for vertebrates for mammalian
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962829327P | 2019-04-04 | 2019-04-04 | |
| PCT/US2020/026405 WO2020206134A1 (en) | 2019-04-04 | 2020-04-02 | Methods for scarless introduction of targeted modifications into targeting vectors |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11202108524SA true SG11202108524SA (en) | 2021-09-29 |
Family
ID=70476358
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202108524SA SG11202108524SA (en) | 2019-04-04 | 2020-04-02 | Methods for scarless introduction of targeted modifications into targeting vectors |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US11111504B2 (en) |
| EP (1) | EP3775201B1 (en) |
| JP (1) | JP7065260B2 (en) |
| KR (1) | KR102487901B1 (en) |
| CN (1) | CN113795588A (en) |
| AU (1) | AU2020253531C1 (en) |
| CA (1) | CA3133359C (en) |
| ES (1) | ES2923629T3 (en) |
| IL (1) | IL286917B (en) |
| RU (1) | RU2771374C1 (en) |
| SG (1) | SG11202108524SA (en) |
| WO (1) | WO2020206134A1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7065260B2 (en) | 2019-04-04 | 2022-05-11 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | Methods for Scarless Introduction of Targeted Modifications to Targeting Vectors |
Family Cites Families (69)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6312892B1 (en) | 1996-07-19 | 2001-11-06 | Cornell Research Foundation, Inc. | High fidelity detection of nucleic acid differences by ligase detection reaction |
| US6599692B1 (en) | 1999-09-14 | 2003-07-29 | Sangamo Bioscience, Inc. | Functional genomics using zinc finger proteins |
| US20030104526A1 (en) | 1999-03-24 | 2003-06-05 | Qiang Liu | Position dependent recognition of GNN nucleotide triplets by zinc fingers |
| US6586251B2 (en) | 2000-10-31 | 2003-07-01 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
| US7105348B2 (en) | 2000-10-31 | 2006-09-12 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
| US6596541B2 (en) | 2000-10-31 | 2003-07-22 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
| JP2004537260A (en) | 2000-12-07 | 2004-12-16 | サンガモ バイオサイエンシーズ， インコーポレイテッド | Regulation of angiogenesis by zinc finger proteins |
| CA2435394C (en) | 2001-01-22 | 2018-01-09 | Sangamo Biosciences, Inc. | Modified zinc finger binding proteins |
| WO2002057308A2 (en) | 2001-01-22 | 2002-07-25 | Sangamo Biosciences, Inc. | Zinc finger polypeptides and their use |
| US8106255B2 (en) | 2002-01-23 | 2012-01-31 | Dana Carroll | Targeted chromosomal mutagenasis using zinc finger nucleases |
| US8206965B2 (en) | 2002-03-15 | 2012-06-26 | Cellectis S.A. | Hybrid and single chain meganucleases and use thereof |
| AU2003218382B2 (en) | 2002-03-21 | 2007-12-13 | Sangamo Therapeutics, Inc. | Methods and compositions for using zinc finger endonucleases to enhance homologous recombination |
| AU2003298574B2 (en) | 2002-09-05 | 2008-04-24 | California Institute Of Technology | Use of chimeric nucleases to stimulate gene targeting |
| AU2003290518A1 (en) | 2002-09-06 | 2004-04-23 | Fred Hutchinson Cancer Research Center | Methods and compositions concerning designed highly-specific nucleic acid binding proteins |
| US7888121B2 (en) | 2003-08-08 | 2011-02-15 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
| US8409861B2 (en) | 2003-08-08 | 2013-04-02 | Sangamo Biosciences, Inc. | Targeted deletion of cellular DNA sequences |
| RU2248983C1 (en) * | 2003-08-18 | 2005-03-27 | Позмогова Галина Евгеньевна | Peptide vector, method for its preparing, nucleotide sequence, recombinant plasmid dna and strain escherichia coli b-8389 vkpm for its preparing, method for genetic modification of mammalian and human cells |
| EP1591521A1 (en) | 2004-04-30 | 2005-11-02 | Cellectis | I-Dmo I derivatives with enhanced activity at 37 degrees C and use thereof |
| AU2005287278B2 (en) | 2004-09-16 | 2011-08-04 | Sangamo Biosciences, Inc. | Compositions and methods for protein production |
| WO2006097784A1 (en) | 2005-03-15 | 2006-09-21 | Cellectis | I-crei meganuclease variants with modified specificity, method of preparation and uses thereof |
| ATE466933T1 (en) | 2005-03-15 | 2010-05-15 | Cellectis | I-CREI MEGANUCLEASE VARIANTS WITH MODIFIED SPECIFICITY AND METHOD FOR THEIR PRODUCTION AND USE |
| WO2008076290A2 (en) | 2006-12-14 | 2008-06-26 | Dow Agrosciences Llc | Optimized non-canonical zinc finger proteins |
| US9273310B2 (en) * | 2007-10-08 | 2016-03-01 | Synthetic Genomics, Inc. | Methods for cloning and manipulating genomes |
| DK2255013T3 (en) | 2008-02-15 | 2016-09-12 | Synthetic Genomics Inc | Methods for in vitro joining and combinatorial assembly of nucleic acid molecules. |
| EP2206723A1 (en) | 2009-01-12 | 2010-07-14 | Bonas, Ulla | Modular DNA-binding domains |
| US20110239315A1 (en) | 2009-01-12 | 2011-09-29 | Ulla Bonas | Modular dna-binding domains and methods of use |
| JP5932632B2 (en) | 2009-03-20 | 2016-06-15 | サンガモ バイオサイエンシーズ， インコーポレイテッド | Modification of CXCR4 using modified zinc finger protein |
| US8772008B2 (en) | 2009-05-18 | 2014-07-08 | Sangamo Biosciences, Inc. | Methods and compositions for increasing nuclease activity |
| US20120178647A1 (en) | 2009-08-03 | 2012-07-12 | The General Hospital Corporation | Engineering of zinc finger arrays by context-dependent assembly |
| PL2510096T5 (en) | 2009-12-10 | 2018-06-29 | Regents Of The University Of Minnesota | Tal effector-mediated dna modification |
| US9689012B2 (en) | 2010-10-12 | 2017-06-27 | Cornell University | Method of dual-adapter recombination for efficient concatenation of multiple DNA fragments in shuffled or specified arrangements |
| GB2496849B (en) * | 2011-11-19 | 2016-06-08 | Oxford Genetics Ltd | Improved DNA plasmid system |
| US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| WO2013141680A1 (en) | 2012-03-20 | 2013-09-26 | Vilnius University | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
| EA038924B1 (en) | 2012-05-25 | 2021-11-10 | Те Риджентс Оф Те Юниверсити Оф Калифорния | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| CN105441440B (en) | 2012-10-23 | 2020-12-15 | 基因工具股份有限公司 | Composition for cleaving target DNA comprising guide RNA specific for target DNA and CAS protein-encoding nucleic acid or CAS protein, and use thereof |
| KR102531576B1 (en) | 2012-12-06 | 2023-05-11 | 시그마-알드리치 컴퍼니., 엘엘씨 | Crispr-based genome modification and regulation |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| ES2883590T3 (en) | 2012-12-12 | 2021-12-09 | Broad Inst Inc | Supply, modification and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications |
| RU2766685C2 (en) | 2012-12-17 | 2022-03-15 | Президент Энд Фэллоуз Оф Харвард Коллидж | Rna-guided human genome engineering |
| WO2014131833A1 (en) | 2013-02-27 | 2014-09-04 | Helmholtz Zentrum München Deutsches Forschungszentrum Für Gesundheit Und Umwelt (Gmbh) | Gene editing in the oocyte by cas9 nucleases |
| CN112301024A (en) | 2013-03-15 | 2021-02-02 | 通用医疗公司 | Increasing specificity of RNA-guided genome editing using RNA-guided FokI nuclease (RFN) |
| CN115261411A (en) | 2013-04-04 | 2022-11-01 | 哈佛学院校长同事会 | Therapeutic uses of genome editing with CRISPR/Cas systems |
| WO2015048577A2 (en) | 2013-09-27 | 2015-04-02 | Editas Medicine, Inc. | Crispr-related methods and compositions |
| WO2015116686A1 (en) | 2014-01-29 | 2015-08-06 | Agilent Technologies, Inc. | Cas9-based isothermal method of detection of specific dna sequence |
| ES2784754T3 (en) * | 2014-06-06 | 2020-09-30 | Regeneron Pharma | Methods and compositions to modify a target locus |
| SI3354732T1 (en) | 2014-06-23 | 2020-07-31 | Regeneron Pharmaceuticals, Inc. | Nuclease-mediated dna assembly |
| US20150376587A1 (en) | 2014-06-25 | 2015-12-31 | Caribou Biosciences, Inc. | RNA Modification to Engineer Cas9 Activity |
| US20170198268A1 (en) | 2014-07-09 | 2017-07-13 | Gen9, Inc. | Compositions and Methods for Site-Directed DNA Nicking and Cleaving |
| US20160053272A1 (en) | 2014-07-18 | 2016-02-25 | Whitehead Institute For Biomedical Research | Methods Of Modifying A Sequence Using CRISPR |
| US11071289B2 (en) | 2014-08-14 | 2021-07-27 | Biocytogen Boston Corp | DNA knock-in system |
| EP3450558B1 (en) | 2014-08-27 | 2020-09-30 | New England Biolabs, Inc. | Synthon formation |
| WO2016100389A1 (en) | 2014-12-16 | 2016-06-23 | Synthetic Genomics, Inc. | Compositions of and methods for in vitro viral genome engineering |
| CN107208113A (en) * | 2014-12-19 | 2017-09-26 | 瑞泽恩制药公司 | Method and composition for carrying out targeting genetic modification by the multiple targeting of single step |
| WO2016106236A1 (en) | 2014-12-23 | 2016-06-30 | The Broad Institute Inc. | Rna-targeting system |
| WO2016109255A1 (en) | 2014-12-30 | 2016-07-07 | University Of South Florida | Methods and compositions for cloning into large vectors |
| WO2016130697A1 (en) | 2015-02-11 | 2016-08-18 | Memorial Sloan Kettering Cancer Center | Methods and kits for generating vectors that co-express multiple target molecules |
| US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| EP3433354B1 (en) * | 2016-03-23 | 2023-12-27 | Synthetic Genomics, Inc. | Generation of synthetic genomes |
| BR112018069795A2 (en) | 2016-03-30 | 2019-01-29 | Intellia Therapeutics Inc | lipid nanoparticle formulations for crispr / cas components |
| US20190330659A1 (en) | 2016-07-15 | 2019-10-31 | Zymergen Inc. | Scarless dna assembly and genome editing using crispr/cpf1 and dna ligase |
| US20180105806A1 (en) | 2016-09-07 | 2018-04-19 | Massachusetts Institute Of Technology | Method for rna-guided endonuclease-based dna assembly |
| EP3551757A1 (en) | 2016-12-08 | 2019-10-16 | Intellia Therapeutics, Inc. | Modified guide rnas |
| JP7308380B2 (en) | 2017-01-10 | 2023-07-14 | クリスティアーナ ケア ジーン エディティング インスティテュート，インコーポレイテッド | Methods for in vitro site-directed mutagenesis using gene editing technology |
| CN110312797A (en) | 2017-02-10 | 2019-10-08 | 齐默尔根公司 | The modular universal plasmid layout strategy of assembling and editor for multiple DNA constructs of multiple hosts |
| JP7466905B2 (en) * | 2017-07-18 | 2024-04-15 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | Scarless genome editing by two-step homology-directed repair |
| JP7025552B2 (en) | 2018-07-30 | 2022-02-24 | オリシロジェノミクス株式会社 | How to edit DNA in a cell-free system |
| JP2021533773A (en) | 2018-08-15 | 2021-12-09 | ザイマージェン インコーポレイテッド | Application of CRISPRi in high-throughput metabolic operations |
| JP7065260B2 (en) | 2019-04-04 | 2022-05-11 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | Methods for Scarless Introduction of Targeted Modifications to Targeting Vectors |
-
2020
- 2020-04-02 JP JP2021550180A patent/JP7065260B2/en active Active
- 2020-04-02 US US16/838,621 patent/US11111504B2/en active Active
- 2020-04-02 CA CA3133359A patent/CA3133359C/en active Active
- 2020-04-02 AU AU2020253531A patent/AU2020253531C1/en active Active
- 2020-04-02 KR KR1020217027439A patent/KR102487901B1/en active IP Right Grant
- 2020-04-02 IL IL286917A patent/IL286917B/en unknown
- 2020-04-02 RU RU2021126206A patent/RU2771374C1/en active
- 2020-04-02 CN CN202080018518.1A patent/CN113795588A/en active Pending
- 2020-04-02 WO PCT/US2020/026405 patent/WO2020206134A1/en unknown
- 2020-04-02 EP EP20722744.8A patent/EP3775201B1/en active Active
- 2020-04-02 SG SG11202108524SA patent/SG11202108524SA/en unknown
- 2020-04-02 ES ES20722744T patent/ES2923629T3/en active Active
-
2021
- 2021-04-30 US US17/245,437 patent/US11499164B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2022522452A (en) | 2022-04-19 |
| JP7065260B2 (en) | 2022-05-11 |
| AU2020253531B2 (en) | 2022-03-17 |
| RU2771374C1 (en) | 2022-05-04 |
| ES2923629T3 (en) | 2022-09-29 |
| US11111504B2 (en) | 2021-09-07 |
| CA3133359A1 (en) | 2020-10-08 |
| KR20210148089A (en) | 2021-12-07 |
| CA3133359C (en) | 2023-04-11 |
| US20210254099A1 (en) | 2021-08-19 |
| EP3775201B1 (en) | 2022-06-15 |
| US20200318134A1 (en) | 2020-10-08 |
| KR102487901B1 (en) | 2023-01-12 |
| EP3775201A1 (en) | 2021-02-17 |
| AU2020253531A1 (en) | 2021-09-02 |
| CN113795588A (en) | 2021-12-14 |
| WO2020206134A1 (en) | 2020-10-08 |
| IL286917A (en) | 2021-10-31 |
| WO2020206134A9 (en) | 2020-10-29 |
| IL286917B (en) | 2022-09-01 |
| AU2020253531C1 (en) | 2022-06-16 |
| US11499164B2 (en) | 2022-11-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IL284651B (en) | 3' utr sequences for stabilization of rna | |
| SG11202001208XA (en) | Rna targeting methods and compositions | |
| IL281586A (en) | Compositions and methods for manufacturing gene therapy vectors | |
| IL265953A (en) | Targeted gene insertion for improved immune cells therapy | |
| IL254811A0 (en) | Compositions and methods of targeting mutant k-ras | |
| IL272942A (en) | Method of targeting exosomes | |
| ZA201803723B (en) | Compositions and methods for efficient targeting of transgenes | |
| IL273841A (en) | Antibodies targeting cd137 and methods of use thereof | |
| IL268912A (en) | Gene therapy constructs and methods for treatment of hearing loss | |
| GB202309871D0 (en) | Compositions and methods for the targeting of bcl11a | |
| EP4077370A4 (en) | Manabodies targeting tumor antigens and methods of using | |
| EP3069606A4 (en) | Interleukin 2 receptor gamma gene targeting vector, production of immune cell-deficient transgenic cloned mini pig having vector introduced therein, preparation method therefor and use thereof | |
| SG11202101819QA (en) | Gene therapy for the treatment of galactosemia | |
| IL286917A (en) | Methods for scarless introduction of targeted modifications into targeting vectors | |
| IL286560A (en) | Methods of preparing cell-binding agent-drug conjugates | |
| GB202014751D0 (en) | Targeting vector | |
| EP3787693A4 (en) | Methods of gene therapy | |
| IL281767A (en) | Antibodies targeting cd137 and methods of use thereof | |
| SG11202102582TA (en) | Method and system of targeting epitopes for neoantigen-based immunotherapy | |
| EP4064827A4 (en) | Methods of transformation | |
| GB201711065D0 (en) | Method of testing a gene therapy vector |