SG11202108524SA - Methods for scarless introduction of targeted modifications into targeting vectors - Google Patents
Methods for scarless introduction of targeted modifications into targeting vectorsInfo
- Publication number
- SG11202108524SA SG11202108524SA SG11202108524SA SG11202108524SA SG11202108524SA SG 11202108524S A SG11202108524S A SG 11202108524SA SG 11202108524S A SG11202108524S A SG 11202108524SA SG 11202108524S A SG11202108524S A SG 11202108524SA SG 11202108524S A SG11202108524S A SG 11202108524SA
- Authority
- SG
- Singapore
- Prior art keywords
- scarless
- introduction
- methods
- targeting vectors
- targeted modifications
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/106—Plasmid DNA for vertebrates
- C12N2800/107—Plasmid DNA for vertebrates for mammalian
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Thermotherapy And Cooling Therapy Devices (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962829327P | 2019-04-04 | 2019-04-04 | |
| PCT/US2020/026405 WO2020206134A1 (en) | 2019-04-04 | 2020-04-02 | Methods for scarless introduction of targeted modifications into targeting vectors |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11202108524SA true SG11202108524SA (en) | 2021-09-29 |
Family
ID=70476358
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202108524SA SG11202108524SA (en) | 2019-04-04 | 2020-04-02 | Methods for scarless introduction of targeted modifications into targeting vectors |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US11111504B2 (en) |
| EP (1) | EP3775201B1 (en) |
| JP (1) | JP7065260B2 (en) |
| KR (1) | KR102487901B1 (en) |
| CN (1) | CN113795588A (en) |
| AU (1) | AU2020253531C1 (en) |
| CA (1) | CA3133359C (en) |
| ES (1) | ES2923629T3 (en) |
| IL (1) | IL286917B (en) |
| RU (1) | RU2771374C1 (en) |
| SG (1) | SG11202108524SA (en) |
| WO (1) | WO2020206134A1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3775201B1 (en) | 2019-04-04 | 2022-06-15 | Regeneron Pharmaceuticals, Inc. | Methods for scarless introduction of targeted modifications into targeting vectors |
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| US6596541B2 (en) | 2000-10-31 | 2003-07-22 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
| AU2884102A (en) | 2000-12-07 | 2002-06-18 | Sangamo Biosciences Inc | Regulation of angiogenesis with zinc finger proteins |
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| AU2003290518A1 (en) | 2002-09-06 | 2004-04-23 | Fred Hutchinson Cancer Research Center | Methods and compositions concerning designed highly-specific nucleic acid binding proteins |
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-
2020
- 2020-04-02 EP EP20722744.8A patent/EP3775201B1/en active Active
- 2020-04-02 US US16/838,621 patent/US11111504B2/en active Active
- 2020-04-02 ES ES20722744T patent/ES2923629T3/en active Active
- 2020-04-02 SG SG11202108524SA patent/SG11202108524SA/en unknown
- 2020-04-02 CN CN202080018518.1A patent/CN113795588A/en active Pending
- 2020-04-02 KR KR1020217027439A patent/KR102487901B1/en active IP Right Grant
- 2020-04-02 JP JP2021550180A patent/JP7065260B2/en active Active
- 2020-04-02 RU RU2021126206A patent/RU2771374C1/en active
- 2020-04-02 IL IL286917A patent/IL286917B/en unknown
- 2020-04-02 WO PCT/US2020/026405 patent/WO2020206134A1/en unknown
- 2020-04-02 CA CA3133359A patent/CA3133359C/en active Active
- 2020-04-02 AU AU2020253531A patent/AU2020253531C1/en active Active
-
2021
- 2021-04-30 US US17/245,437 patent/US11499164B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CA3133359C (en) | 2023-04-11 |
| AU2020253531A1 (en) | 2021-09-02 |
| WO2020206134A1 (en) | 2020-10-08 |
| US20200318134A1 (en) | 2020-10-08 |
| IL286917A (en) | 2021-10-31 |
| IL286917B (en) | 2022-09-01 |
| EP3775201B1 (en) | 2022-06-15 |
| AU2020253531B2 (en) | 2022-03-17 |
| EP3775201A1 (en) | 2021-02-17 |
| JP2022522452A (en) | 2022-04-19 |
| CN113795588A (en) | 2021-12-14 |
| RU2771374C1 (en) | 2022-05-04 |
| US11499164B2 (en) | 2022-11-15 |
| JP7065260B2 (en) | 2022-05-11 |
| CA3133359A1 (en) | 2020-10-08 |
| US11111504B2 (en) | 2021-09-07 |
| ES2923629T3 (en) | 2022-09-29 |
| WO2020206134A9 (en) | 2020-10-29 |
| KR102487901B1 (en) | 2023-01-12 |
| US20210254099A1 (en) | 2021-08-19 |
| KR20210148089A (en) | 2021-12-07 |
| AU2020253531C1 (en) | 2022-06-16 |
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