SG11202102209XA - An improved process for the preparation of recombinant lectin protein - Google Patents

An improved process for the preparation of recombinant lectin protein

Info

Publication number
SG11202102209XA
SG11202102209XA SG11202102209XA SG11202102209XA SG11202102209XA SG 11202102209X A SG11202102209X A SG 11202102209XA SG 11202102209X A SG11202102209X A SG 11202102209XA SG 11202102209X A SG11202102209X A SG 11202102209XA SG 11202102209X A SG11202102209X A SG 11202102209XA
Authority
SG
Singapore
Prior art keywords
preparation
improved process
lectin protein
recombinant lectin
recombinant
Prior art date
Application number
SG11202102209XA
Other languages
English (en)
Inventor
Dhananjay Sathe
Sudeep Kumar
Mamata Katdare
Original Assignee
Unichem Lab Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unichem Lab Ltd filed Critical Unichem Lab Ltd
Publication of SG11202102209XA publication Critical patent/SG11202102209XA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/42—Lectins, e.g. concanavalin, phytohaemagglutinin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4726—Lectins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Botany (AREA)
  • Biomedical Technology (AREA)
  • Water Supply & Treatment (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11202102209XA 2018-09-07 2019-09-05 An improved process for the preparation of recombinant lectin protein SG11202102209XA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IN201821008382 2018-09-07
IN201921001559 2019-01-14
PCT/IB2019/057471 WO2020074977A1 (fr) 2018-09-07 2019-09-05 Procédé amélioré pour la préparation d'une protéine de lectine recombinante

Publications (1)

Publication Number Publication Date
SG11202102209XA true SG11202102209XA (en) 2021-04-29

Family

ID=68159140

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202102209XA SG11202102209XA (en) 2018-09-07 2019-09-05 An improved process for the preparation of recombinant lectin protein

Country Status (13)

Country Link
US (1) US20210324438A1 (fr)
EP (1) EP3847184A1 (fr)
JP (1) JP2021536253A (fr)
KR (1) KR20210058840A (fr)
CN (1) CN113286809A (fr)
AU (1) AU2019358330A1 (fr)
BR (1) BR112021004241A2 (fr)
CA (1) CA3111367A1 (fr)
MA (1) MA53554A (fr)
MX (1) MX2021002672A (fr)
SG (1) SG11202102209XA (fr)
WO (1) WO2020074977A1 (fr)
ZA (1) ZA202101433B (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4366699A1 (fr) 2021-07-08 2024-05-15 Unichem Laboratories Ltd Protéines recombinantes, compositions et procédés de stabilisation de celles-ci
CA3229988A1 (fr) 2021-10-01 2023-04-06 Dhananjay Sathe Proteines de liaison au glycane recombinantes et leur utilisation

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100339475C (zh) * 2003-08-14 2007-09-26 海南大学 用于重组生产雪花莲外源凝集素的大肠杆菌菌株及方法
EP3418296B1 (fr) * 2009-02-18 2024-07-03 Unichem Laboratories Limited Lectines recombinantes se liant à des cellules cancéreuses dotées d'une activité antitumorale et procédé de préparation
WO2014203261A2 (fr) * 2010-02-02 2014-12-24 Unichem Laboratories Ltd. Lectine recombinée et son procédé de préparation
EP3153581B1 (fr) * 2014-06-09 2019-08-28 Kaneka Corporation Procédé de production de protéines recombinées à l'aide de brevibacillus recombiné

Also Published As

Publication number Publication date
KR20210058840A (ko) 2021-05-24
AU2019358330A1 (en) 2021-04-08
BR112021004241A2 (pt) 2021-05-25
CN113286809A (zh) 2021-08-20
CA3111367A1 (fr) 2020-04-16
US20210324438A1 (en) 2021-10-21
EP3847184A1 (fr) 2021-07-14
ZA202101433B (en) 2022-07-27
MX2021002672A (es) 2021-06-23
WO2020074977A1 (fr) 2020-04-16
JP2021536253A (ja) 2021-12-27
WO2020074977A9 (fr) 2020-08-06
MA53554A (fr) 2021-07-14

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