SG11201909983QA - Method of determining expression level of t315i variant of abl1 - Google Patents
Method of determining expression level of t315i variant of abl1Info
- Publication number
- SG11201909983QA SG11201909983QA SG11201909983QA SG11201909983QA SG 11201909983Q A SG11201909983Q A SG 11201909983QA SG 11201909983Q A SG11201909983Q A SG 11201909983QA SG 11201909983Q A SG11201909983Q A SG 11201909983QA
- Authority
- SG
- Singapore
- Prior art keywords
- abl1
- reverse
- primer
- variant
- expression level
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Biomedical Technology (AREA)
- Plant Pathology (AREA)
Abstract
OF DISCLOSURE The disclosure provides .a method comprising () reverse- transcribing an RNA sample obtained from the subject in the presence of .a modified nucleic acid complementary to a Tegion comprising the position of the.T315I mutation of a wild-type ABL1 mRNA'by using (a) a reverse-primer that hybridizes to an ABL1 mRNA in a region downstream from the position of the T315I mutation and (b) a reverse primer that hybridizes to an ABL1 mRNA in a region upstream from the position of the T315I mutation in one container; and (2) calculating the expression level of the T315I variant of ABL1 on the basis of the ratio of the reverse transcript generated with the primer (a) to the reverse transcript'generated with the primer (b), as well as a kit for condtcting the method. 46
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2017087578 | 2017-04-26 | ||
PCT/JP2018/016748 WO2018199136A1 (en) | 2017-04-26 | 2018-04-25 | Method for measuring expression level of abl1 t315i mutation |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11201909983QA true SG11201909983QA (en) | 2019-11-28 |
Family
ID=63919184
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG10201912849TA SG10201912849TA (en) | 2017-04-26 | 2018-04-25 | Method of determining expression level of t315i variant of abl1 |
SG11201909983Q SG11201909983QA (en) | 2017-04-26 | 2018-04-25 | Method of determining expression level of t315i variant of abl1 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG10201912849TA SG10201912849TA (en) | 2017-04-26 | 2018-04-25 | Method of determining expression level of t315i variant of abl1 |
Country Status (6)
Country | Link |
---|---|
JP (1) | JPWO2018199136A1 (en) |
KR (1) | KR20200002933A (en) |
CN (1) | CN110997938A (en) |
PH (1) | PH12019502417A1 (en) |
SG (2) | SG10201912849TA (en) |
WO (1) | WO2018199136A1 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111321200A (en) * | 2020-02-28 | 2020-06-23 | 广州安镝声生物医药科技有限公司 | Extracellular ABL1 kinase activity detection kit and application thereof |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030211483A1 (en) * | 2002-05-09 | 2003-11-13 | Schroeder Benjamin G. | Methods for the enrichment of low-abundance polynucleotides |
JP5221248B2 (en) * | 2008-08-26 | 2013-06-26 | 株式会社日立ハイテクノロジーズ | Method for preparing a cDNA library with a reduced content of cDNA clones derived from highly expressed genes |
TWI541507B (en) * | 2009-09-11 | 2016-07-11 | 香港中文大學 | Methods for assessing liver pathologies |
KR101875199B1 (en) * | 2010-11-11 | 2018-07-06 | 주식회사 파나진 | Methods and kits for the detection of BCR-ABL fusion gene mutant using PNA mediated Real-time PCR clamping |
CN102676638B (en) * | 2011-03-08 | 2014-06-04 | 苏州大学附属第一医院 | Method and kit for detecting drug-resistance mutation site of ABL kinase domain of BCR/ABL fusion gene |
JP6531312B2 (en) * | 2014-10-17 | 2019-06-19 | 東洋鋼鈑株式会社 | Method for detecting BCR-ABL inhibitor resistance related mutation and data acquisition method for predicting BCR-ABL inhibitor resistance using the same |
CN105349681A (en) * | 2015-12-07 | 2016-02-24 | 湖南圣维基因科技有限公司 | BCR-ABL fusion gene T315I mutation fluorescent PCR detection kit |
RU2609641C1 (en) * | 2015-12-07 | 2017-02-02 | Федеральное Государственное Бюджетное Учреждение Науки Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук (Имб Ран) | Method of analysis of somatic mutations in the bcr/abl chimeric gene using rt-pcr and subsequent hybridization with oligonucleotide biological microchip (biochip) |
CN105441568B (en) * | 2016-01-06 | 2019-01-08 | 武汉海吉力生物科技有限公司 | For detecting primer, probe and the kit of mankind BCR-ABL fusion T315I mutation |
-
2018
- 2018-04-25 JP JP2019514562A patent/JPWO2018199136A1/en active Pending
- 2018-04-25 KR KR1020197034072A patent/KR20200002933A/en unknown
- 2018-04-25 SG SG10201912849TA patent/SG10201912849TA/en unknown
- 2018-04-25 CN CN201880042856.1A patent/CN110997938A/en active Pending
- 2018-04-25 SG SG11201909983Q patent/SG11201909983QA/en unknown
- 2018-04-25 WO PCT/JP2018/016748 patent/WO2018199136A1/en active Application Filing
-
2019
- 2019-10-25 PH PH12019502417A patent/PH12019502417A1/en unknown
Also Published As
Publication number | Publication date |
---|---|
KR20200002933A (en) | 2020-01-08 |
PH12019502417A1 (en) | 2020-10-19 |
SG10201912849TA (en) | 2020-02-27 |
WO2018199136A1 (en) | 2018-11-01 |
CN110997938A (en) | 2020-04-10 |
JPWO2018199136A1 (en) | 2020-05-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
MX2021011031A (en) | Fed-batch in vitro transcription process. | |
EP4328322A3 (en) | Probe library construction | |
MX2019005199A (en) | Methods for assessing risk using total and specific cell-free dna. | |
SG10201908277TA (en) | Method of determining the risk of developing breast cancer by detecting the expression levels of micrornas (mirnas) | |
SG10201804519RA (en) | Methods and systems for detecting genetic variants | |
EP4306659A3 (en) | A method and kit for determining the tissue or cell origin of dna | |
WO2014052260A3 (en) | Systems, methods and interfaces for evaluating an online entity presence | |
MX344181B (en) | Determining liquid phase turbidity of multiphase wastewater. | |
EP4269610A3 (en) | Probe and method for detecting transcript resulting from fusion gene and/or exon skipping | |
NZ725789A (en) | Dre4 nucleic acid molecules that confer resistance to coleopteran pests | |
MX2016008261A (en) | Ras opposite (rop) and related nucleic acid molecules that confer resistance to coleopteran and/or hemipteran pests. | |
BR112017007253A2 (en) | system and method for ammonia oxidation | |
MX2017007534A (en) | Parental rnai suppression of chromatin remodeling genes to control coleopteran pests. | |
EP4265740A3 (en) | Methods for predicting risk of metastasis in cutaneous melanoma | |
MX2016008263A (en) | Rnapii-140 nucleic acid molecules that confer resistance to coleopteran pests. | |
PH12017501090A1 (en) | Parental rnai suppression of kruppel gene to control coleopteran pests | |
MX2020002826A (en) | Detection of recombinase polymerase amplification using dual-hapten probe. | |
MX2017011445A (en) | Rna polymerase ii33 nucleic acid molecules to control insect pests. | |
SG11201909983QA (en) | Method of determining expression level of t315i variant of abl1 | |
EP4012049A3 (en) | Methods for determining responsiveness to mek/erk inhibitors | |
WO2017074780A3 (en) | Nucleic acid molecules that confer resistance to coleopteran pests | |
WO2013088140A3 (en) | Tenascin-c and use thereof in rheumatoid arthritis | |
NZ603088A (en) | Methods for identifying compositions that alter wildtype expression of genes and proteins in a plant cell | |
MX2017007536A (en) | Parental rnai suppression of kruppel gene to control hemipteran pests. | |
Kondrlová et al. | GIS application in sediment delivery estimation: comparison of two model approaches |