SG11201909712TA - Ketoreductase polypeptides and polynucleotides - Google Patents

Ketoreductase polypeptides and polynucleotides

Info

Publication number
SG11201909712TA
SG11201909712TA SG11201909712TA SG11201909712TA SG 11201909712T A SG11201909712T A SG 11201909712TA SG 11201909712T A SG11201909712T A SG 11201909712TA SG 11201909712T A SG11201909712T A SG 11201909712TA
Authority
SG
Singapore
Prior art keywords
redwood city
international
rahway
phosphite dehydrogenase
penobscot
Prior art date
Application number
Inventor
Jeffrey Moore
Jack Liang
Jonathan Penfield
Jovana Nazor
Nikki Dellas
Vesna Mitchell
Da Duan
Iman Farasat
Agustina Rodriguez-Granillo
Grant Murphy
Nicholas Marshall
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of SG11201909712TA publication Critical patent/SG11201909712TA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/002—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by oxidation/reduction reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/182—Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01002—Alcohol dehydrogenase (NADP+) (1.1.1.2), i.e. aldehyde reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01184—Carbonyl reductase (NADPH) (1.1.1.184)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y120/00—Oxidoreductases acting on phosphorus or arsenic in donors (1.20)
    • C12Y120/01—Oxidoreductases acting on phosphorus or arsenic in donors (1.20) with NAD+ or NADP+ as acceptor (1.20.1)
    • C12Y120/01001—Phosphonate dehydrogenase (1.20.1.1)

Abstract

selective oxidation 2. selective reduction NAD S-selective NADH KRED-NADH-102 R-selective KRED-101 NADP NADPH NADH Oxidase H2O - 02 (1a) .0 , 110H (1) (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 01 November 2018 (01.11.2018) WIPO I PCT o iimion ono o oloim imio (10) International Publication Number WO 2018/200214 A2 (51) International Patent Classification: C12P 7/04 (2006.01) C12N 9/00 (2006.01) (21) International Application Number: PCT/US2018/027450 (22) International Filing Date: 13 April 2018 (13.04.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/491,161 27 April 2017 (27.04.2017) US (71) Applicant: CODEXIS, INC. [US/US]; 200 Penobscot Dri- ve, Redwood City, CA 94063 (US). (72) Inventors: MOORE, Jeffrey, C.; 126 East Lincoln Av- enue, Rahway, NJ 07065 (US). LIANG, Jack; 200 Penob- scot Drive, Redwood City, CA 94063 (US). PENFIELD, Jonathan; 11269 Skyline Ct., Truckee, CA 96161 (US). NAZOR, Jovana; 200 Penobscot Drive, Redwood City, CA 94063 (US). DELLAS, Nikki; 200 Penobscot Drive, Redwood City, CA 94063 (US). MITCHELL, Vesna; 200 Penobscot Drive, Redwood City, CA 94063 (US). DUAN, Da; 200 Penobscot Drive, Redwood City, CA 94063 (US). FARASAT, Iman; 126 East Lincoln Avenue, Rahway, NJ 07065 (US). RODRIGUEZ-GRANILLO, Agustina; 126 East Lincoln Avenue, Rahway, NJ 07065 (US). MURPHY, Grant; 126 East Lincoln Avenue, Rahway, NJ 07065 (US). MARSHAL, Nicholas; 126 East Lincoln Avenue, Rahway, NJ 07065 (US). (74) Agent: MACKNIGHT, Kamrin, T.; Codexis, Inc., 200 Penobscot Drive, Redwood City, CA 94063 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, = (54) Title: KETOREDUCTASE POLYPEPTIDES AND POLYNUCLEOTIDES OH Na2PO4 Na2P03 phosphite dehydrogenase PDH-101 FIG. 1 W O 20 18/ 200214 A2 (57) : The present invention provides engineered ketoreductase and phosphite dehydrogenase enzymes having improved prop- erties as compared to a naturally occurring wild-type ketoreductase and phosphite dehydrogenase enzymes, as well as polynucleotides encoding the engineered ketoreductase and phosphite dehydrogenase enzymes, host cells capable of expressing the engineered ketore- ductase and phosphite dehydrogenase enzymes, and methods of using the engineered ketoreductase and phosphite dehydrogenase en- zymes to synthesize a chiral catalyst used in the synthesis of antiviral compounds, such as nucleoside inhibitors. The present invention further provides methods of using the engineered enzymes to deracemize a chiral alcohol in a one-pot, multi-enzyme system. [Continued on next page] WO 2018/200214 A2 IMIOHOIMIREIHHIMINIRIlinThin KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: without international search report and to be republished upon receipt of that report (Rule 48.2(g)) with sequence listing part of description (Rule 5.2(a))
SG11201909712T 2017-04-27 2018-04-13 Ketoreductase polypeptides and polynucleotides SG11201909712TA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762491161P 2017-04-27 2017-04-27
PCT/US2018/027450 WO2018200214A2 (en) 2017-04-27 2018-04-13 Ketoreductase polypeptides and polynucleotides

Publications (1)

Publication Number Publication Date
SG11201909712TA true SG11201909712TA (en) 2019-11-28

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Family Applications (1)

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SG11201909712T SG11201909712TA (en) 2017-04-27 2018-04-13 Ketoreductase polypeptides and polynucleotides

Country Status (7)

Country Link
US (3) US11021729B2 (en)
EP (1) EP3615058A4 (en)
JP (1) JP7045725B2 (en)
CN (2) CN110831618B (en)
CA (1) CA3061133A1 (en)
SG (1) SG11201909712TA (en)
WO (1) WO2018200214A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111718913B (en) * 2019-03-20 2023-06-20 尚科生物医药（上海）有限公司 Ketoreductase polypeptide and method for preparing (S) -1- (2-iodo-5-fluorophenyl) ethanol by catalyzing same
JP7375052B2 (en) * 2019-06-13 2023-11-07 アシムケム ラボラトリーズ （ティエンジン） カンパニー リミテッド Ketoreductase mutant and method for producing chiral alcohol
CN111394324B (en) * 2020-06-08 2020-09-08 凯莱英生命科学技术(天津)有限公司 Ketoreductase mutant and application thereof
CN112941043B (en) * 2021-05-17 2021-09-10 中国科学院天津工业生物技术研究所 Carbonyl reductase mutant and application thereof in preparation of chiral beta' -hydroxy-beta-amino acid ester
CN115851635A (en) * 2021-08-10 2023-03-28 深圳瑞德林生物技术有限公司 Preparation method of S-nicotine
WO2024010785A1 (en) * 2022-07-08 2024-01-11 Merck Sharp & Dohme Llc Ketoreductase enzymes for the synthesis of 1,3-diol substituted indanes

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CA3061133A1 (en) 2018-11-01
JP2020517284A (en) 2020-06-18
JP7045725B2 (en) 2022-04-01
CN110831618A (en) 2020-02-21
US20210254119A1 (en) 2021-08-19
CN117511890A (en) 2024-02-06
US11746369B2 (en) 2023-09-05
CN110831618B (en) 2023-08-25
WO2018200214A3 (en) 2019-11-28
EP3615058A2 (en) 2020-03-04
EP3615058A4 (en) 2021-06-02
WO2018200214A2 (en) 2018-11-01
US11021729B2 (en) 2021-06-01
US20240068005A1 (en) 2024-02-29
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