SG11201908777PA - Method for rapidly constructing amplicon library through one-step process - Google Patents
Method for rapidly constructing amplicon library through one-step processInfo
- Publication number
- SG11201908777PA SG11201908777PA SG11201908777PA SG11201908777PA SG 11201908777P A SG11201908777P A SG 11201908777PA SG 11201908777P A SG11201908777P A SG 11201908777PA SG 11201908777P A SG11201908777P A SG 11201908777PA
- Authority
- SG
- Singapore
- Prior art keywords
- step process
- amplicon library
- rapidly constructing
- constructing
- rapidly
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710218529.4A CN106835292B (en) | 2017-04-05 | 2017-04-05 | The method of one-step method rapid build amplification sublibrary |
| PCT/CN2018/080864 WO2018184495A1 (en) | 2017-04-05 | 2018-03-28 | Method for constructing amplicon library through one-step process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201908777PA true SG11201908777PA (en) | 2019-10-30 |
Family
ID=59142887
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201908777P SG11201908777PA (en) | 2017-04-05 | 2018-03-28 | Method for rapidly constructing amplicon library through one-step process |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US11155862B2 (en) |
| EP (1) | EP3608452A4 (en) |
| JP (1) | JP2020512405A (en) |
| KR (1) | KR102204274B1 (en) |
| CN (1) | CN106835292B (en) |
| SG (1) | SG11201908777PA (en) |
| WO (1) | WO2018184495A1 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106835292B (en) * | 2017-04-05 | 2019-04-09 | 北京泛生子基因科技有限公司 | The method of one-step method rapid build amplification sublibrary |
| CN107604045A (en) * | 2017-10-19 | 2018-01-19 | 北京泛生子基因科技有限公司 | A kind of construction method of amplification sublibrary for the mutation of testing goal gene low frequency |
| CN112301430B (en) * | 2019-07-30 | 2022-05-17 | 北京泛生子基因科技有限公司 | Library building method and application |
| CN112322704B (en) * | 2020-12-02 | 2021-07-20 | 武汉艾迪晶生物科技有限公司 | Method for rapidly detecting DNA sequence mutation in batches |
| CN113308526B (en) * | 2021-07-13 | 2022-04-08 | 北京爱普益生物科技有限公司 | Fusion primer direct amplification method human mitochondrial whole genome high-throughput sequencing kit |
| CN117661123A (en) * | 2023-12-02 | 2024-03-08 | 云准医药科技(广州)有限公司 | Single-cell library construction method for multiple sample mixing |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EA023190B9 (en) * | 2009-04-02 | 2018-03-30 | Флуидигм Корпорейшн | Multi-primer amplification method for barcoding of target nucleic acids |
| EP2559774A1 (en) * | 2011-08-17 | 2013-02-20 | Roche Diagniostics GmbH | Improved method for amplification of target nucleic acids using a multi-primer approach |
| US10138519B2 (en) * | 2012-12-28 | 2018-11-27 | Quest Diagnostics Investments Incorporated | Universal sanger sequencing from next-gen sequencing amplicons |
| JP2016515380A (en) * | 2013-03-15 | 2016-05-30 | ライフ テクノロジーズ コーポレーション | Lung cancer classification and feasibility index |
| US9708657B2 (en) * | 2013-07-01 | 2017-07-18 | Adaptive Biotechnologies Corp. | Method for generating clonotype profiles using sequence tags |
| CN103555846B (en) * | 2013-11-07 | 2015-12-30 | 中国海洋大学 | A kind of high-throughput low cost S NP classifying method based on liquid phase molecule Hybridization principle |
| EP3108009B1 (en) * | 2014-02-18 | 2021-03-24 | Illumina, Inc. | Methods and compositions for dna profiling |
| US9745611B2 (en) * | 2014-05-06 | 2017-08-29 | Genewiz Inc. | Methods and kits for identifying microorganisms in a sample |
| CN104263818B (en) * | 2014-09-02 | 2016-06-01 | 武汉凯吉盈科技有限公司 | Based on the whole blood immunity group storehouse detection method of high throughput sequencing technologies |
| AU2015319806A1 (en) | 2014-09-26 | 2017-04-20 | The Regents Of The University Of California | Methods and systems for detection of a genetic mutation |
| RU2017131622A (en) * | 2015-02-13 | 2019-03-13 | Абхиджит Аджит ПАТЕЛ | METHODS FOR HIGH-PARALLEL AND PRECISE MEASUREMENT OF NUCLEIC ACIDS |
| CN105442054B (en) * | 2015-11-19 | 2018-04-03 | 杭州谷坤生物技术有限公司 | The method that storehouse is built in the amplification of multiple target site is carried out to plasma DNA |
| CN106011230A (en) * | 2016-05-10 | 2016-10-12 | 人和未来生物科技（长沙）有限公司 | Primer composition for detecting fragmentized DNA target area and application thereof |
| CN106048008A (en) * | 2016-06-03 | 2016-10-26 | 人和未来生物科技（长沙）有限公司 | EGFR gene ultralow frequency mutation detection kit |
| CN105861724B (en) * | 2016-06-03 | 2019-07-16 | 人和未来生物科技（长沙）有限公司 | A kind of KRAS gene ultralow frequency mutation detection kit |
| CN106319064A (en) | 2016-09-13 | 2017-01-11 | 北京天科雅生物科技有限公司 | Multi-PCR-primer and method for constructing human TCRA library on basis of high-throughput sequencing |
| CN106906210A (en) | 2017-04-05 | 2017-06-30 | 北京泛生子医学检验实验室有限公司 | A kind of fusion primer combination of rapid build amplification sublibrary |
| CN106834286B (en) | 2017-04-05 | 2020-02-21 | 北京泛生子基因科技有限公司 | Primer combination for one-step method rapid construction of amplicon library |
| CN106835292B (en) | 2017-04-05 | 2019-04-09 | 北京泛生子基因科技有限公司 | The method of one-step method rapid build amplification sublibrary |
| CN107604045A (en) | 2017-10-19 | 2018-01-19 | 北京泛生子基因科技有限公司 | A kind of construction method of amplification sublibrary for the mutation of testing goal gene low frequency |
-
2017
- 2017-04-05 CN CN201710218529.4A patent/CN106835292B/en active Active
-
2018
- 2018-03-28 WO PCT/CN2018/080864 patent/WO2018184495A1/en unknown
- 2018-03-28 EP EP18780976.9A patent/EP3608452A4/en active Pending
- 2018-03-28 KR KR1020197024150A patent/KR102204274B1/en active IP Right Grant
- 2018-03-28 JP JP2020502754A patent/JP2020512405A/en active Pending
- 2018-03-28 US US16/481,938 patent/US11155862B2/en active Active
- 2018-03-28 SG SG11201908777P patent/SG11201908777PA/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US11155862B2 (en) | 2021-10-26 |
| KR102204274B1 (en) | 2021-01-15 |
| WO2018184495A1 (en) | 2018-10-11 |
| KR20190104611A (en) | 2019-09-10 |
| CN106835292A (en) | 2017-06-13 |
| EP3608452A1 (en) | 2020-02-12 |
| EP3608452A4 (en) | 2021-01-13 |
| JP2020512405A (en) | 2020-04-23 |
| US20190352711A1 (en) | 2019-11-21 |
| CN106835292B (en) | 2019-04-09 |
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