SG11201908376UA - Methods and compositions for inducing immune responses against clostridium difficile - Google Patents

Abstract

MolWE: ,312 Mature CDTb (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 20 September 2018 (20.09.2018) WIP0 I PCT Iiiimmomiolollmonolomolomolimillomoiflomom (10) International Publication Number WO 2018/170238 A2 (51) International Patent Classification: A61K 39/08 (2006.01) (21) International Application Number: PCT/US2018/022597 (22) International Filing Date: 15 March 2018 (15.03.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/471,636 15 March 2017 (15.03.2017) US 62/474,434 21 March 2017 (21.03.2017) US (71) Applicant: NOVAVAX, INC. [US/US]; 20 Firstfield Road, Gaithersburg, Maryland 20878 (US). (72) Inventors: TIAN, Ting-Hui; c/o Novavax, Inc., 20 First- field Road, Gaithersburg, Maryland 20878 (US). LIU, Ye; c/o Novavax, Inc., 20 Firstfield Road, Gaithersburg, Maryland 20878 (US). SMITH, Gale; c/o Novavax, Inc., 20 Firstfield Road, Gaithersburg, Maryland 20878 (US). GLENN, Gregory; c/o Novavax, Inc., 20 Firstfield Road, Gaithersburg, Maryland 20878 (US). FLYER, David; c/o Novavax, Inc., 20 Firstfield Road, Gaithersburg, Maryland 20878 (US). (74) Agent: TUSCAN, Michael et al.; Cooley LLP, 1299 Penn- sylvania Avenue, N.W., Suite 700, Washington, District of Columbia 20004-2400 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (54) Title: METHODS AND COMPOSITIONS FOR INDUCING IMMUNE RESPONSES AGAINST CLOSTRIDIUM DIFFICILE Fig. 1: C. difficile triple toxin vaccine constructs Triple toxin 1420 CDTb Mature CDTb Tcca RBD cc O N 00 O C iRARRRKKR With a Turin cleavage site Mal Wt: 206,421 Triple toxin 1470 (57) : Disclosed herein are methods and compositions for treating or preventing bacterial infection. In particular, the methods and compositions are directed towards C. difficile infection. In particular aspects, the compositions are vaccines containing multimeric polypeptides containing portions of multiple toxins from bacteria. The polypeptides induce effective immune responses thus treating or preventing infection. [Continued on next page] WO 2018/170238 A2 IMIOH01101 DIVIRE1013311011010111OH11111i# TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(11)) Published: without international search report and to be republished upon receipt of that report (Rule 48.2(g)) with sequence listing part of description (Rule 5.2(a))