SG11201907569SA - Single cell analysis - Google Patents
Single cell analysisInfo
- Publication number
- SG11201907569SA SG11201907569SA SG11201907569SA SG11201907569SA SG11201907569SA SG 11201907569S A SG11201907569S A SG 11201907569SA SG 11201907569S A SG11201907569S A SG 11201907569SA SG 11201907569S A SG11201907569S A SG 11201907569SA SG 11201907569S A SG11201907569S A SG 11201907569SA
- Authority
- SG
- Singapore
- Prior art keywords
- international
- paris
- pct
- english
- march
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0803—Disc shape
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Management, Administration, Business Operations System, And Electronic Commerce (AREA)
Abstract
International Patent Classification: C12Q 1/68 (2018.01) C12N 15/10 (2006.01) (21) International Application Number: PCT/EP2018/056551 (22) International Filing Date: 15 March 2018 (15.03.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 17305297.8 17 March 2017 (17.03.2017) EP MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) (71) Applicant: HIFIBIO SAS HIG FIDELITY BIOLOGY [FR/FR]; Pepiniere Paris Sante Cochin 29, Rue Faubourg Saint-Jacques, 75014 Paris (FR). (72) Inventors: REICHEN, Marcel; Engelstrasse 1, 8820 Wadenswil (CH). REITZ, Arnaud; 1, clos Adrienne Bol- land, 78280 Guyancourt (FR). SHEN, Bingqing; Batiment B1 44, ay. de Paris, 92320 Chatillon (FR). ELLOUZE, Sami; Appartment 9 23 rue des Tournelles, 92290 Chate- nay Malabry (FR). GERARD, Annabelle; 13 impasse de la cerisaie, 91120 Palaiseau (FR). (74) Agent: CH KILGER ANWALTSPARTNERSCHAFT MBB; FasanenstraBe 29, 10719 Berlin (DE). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, Semple droplets (54) Title: SINGLE CELL ANALYSIS elf+ b Y : Ct value X : DNA copies number r'• ei Positive droplet Quantification lard t. F** * Quante* : tr.Jarci Lirt, ,...oncentrationl Pat cYde (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 20 September 2018 (20.09.2018) WI P0 I PCT omit VIII °nolo Oil 1001111 OH °nom° oimIE (10) International Publication Number WO 2018/167218 Al Threshold (Ct) Standard cum droplet FIG. 7 (57) : The present invention concerns processes for barcoding nucleic acids from single cells and processes for genotyping single cells having a phenotype of interest.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP17305297.8A EP3375889B1 (en) | 2017-03-17 | 2017-03-17 | Single cell analysis |
| PCT/EP2018/056551 WO2018167218A1 (en) | 2017-03-17 | 2018-03-15 | Single cell analysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201907569SA true SG11201907569SA (en) | 2019-09-27 |
Family
ID=58448501
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201907569SA SG11201907569SA (en) | 2017-03-17 | 2018-03-15 | Single cell analysis |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US11427853B2 (en) |
| EP (2) | EP3375889B1 (en) |
| JP (1) | JP7050803B2 (en) |
| CN (2) | CN110431239B (en) |
| AU (1) | AU2018234084B2 (en) |
| CA (1) | CA3055005A1 (en) |
| IL (1) | IL269168A (en) |
| LT (2) | LT3375889T (en) |
| SG (1) | SG11201907569SA (en) |
| WO (1) | WO2018167218A1 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG11202107418SA (en) * | 2019-01-06 | 2021-08-30 | 10X Genomics Inc | Methods and systems for enrichment of barcodes |
| CN113767178A (en) | 2019-03-11 | 2021-12-07 | 10X基因组学有限公司 | Systems and methods for processing optically labeled beads |
| WO2021255727A1 (en) * | 2020-06-16 | 2021-12-23 | Onecell Medical Ltd. | Systems and methods for personalized adoptive cell therapy |
| KR102482205B1 (en) * | 2020-11-18 | 2022-12-28 | 주식회사 뉴로소나 | fabrication method for piezoelectric composite |
| WO2022136196A1 (en) | 2020-12-22 | 2022-06-30 | Hifibio | Microfluidic methods and systems |
| WO2023137583A1 (en) * | 2022-01-18 | 2023-07-27 | 深圳华大生命科学研究院 | Method for determining effect relationship between various substances and cells, and microwell array chip |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5412087A (en) | 1992-04-24 | 1995-05-02 | Affymax Technologies N.V. | Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces |
| US5641870A (en) | 1995-04-20 | 1997-06-24 | Genentech, Inc. | Low pH hydrophobic interaction chromatography for antibody purification |
| JP2006507921A (en) | 2002-06-28 | 2006-03-09 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | Method and apparatus for fluid dispersion |
| EP2266687A3 (en) | 2003-04-10 | 2011-06-29 | The President and Fellows of Harvard College | Formation and control of fluidic species |
| EP2662135A3 (en) | 2003-08-27 | 2013-12-25 | President and Fellows of Harvard College | Method for mixing droplets in a microchannel |
| AU2006220816A1 (en) | 2005-03-04 | 2006-09-14 | President And Fellows Of Harvard College | Method and apparatus for forming multiple emulsions |
| US9029085B2 (en) | 2007-03-07 | 2015-05-12 | President And Fellows Of Harvard College | Assays and other reactions involving droplets |
| US9446360B2 (en) | 2009-05-07 | 2016-09-20 | Universite De Strasbourg | Microfluidic system and methods for highly selective droplet fusion |
| EP2608878A4 (en) | 2010-08-23 | 2017-11-15 | President and Fellows of Harvard College | Acoustic waves in microfluidics |
| WO2013177206A2 (en) | 2012-05-21 | 2013-11-28 | Fluidigm Corporation | Single-particle analysis of particle populations |
| EP3089822B1 (en) | 2013-12-30 | 2022-04-06 | Atreca, Inc. | Analysis of nucleic acids associated with single cells using nucleic acid barcodes |
| EP3456846B1 (en) | 2014-04-21 | 2022-06-22 | President and Fellows of Harvard College | Systems and methods for barcoding nucleic acid |
| CN107873054B (en) | 2014-09-09 | 2022-07-12 | 博德研究所 | Droplet-based methods and apparatus for multiplexed single-cell nucleic acid analysis |
| FR3027396B1 (en) | 2014-10-15 | 2016-11-25 | Espci Innov | METHOD FOR ANALYZING DROP CONTENT AND ASSOCIATED APPARATUS |
| CN107614700A (en) | 2015-03-11 | 2018-01-19 | 布罗德研究所有限公司 | Genotype and phenotype coupling |
| EP3303633B1 (en) * | 2015-05-26 | 2024-02-21 | The Trustees of Columbia University in the City of New York | Rna printing and sequencing devices, and systems |
| US10745741B2 (en) * | 2015-06-26 | 2020-08-18 | European Molecular Biology Laboratory | Cell barcoding in microfluidics |
| WO2017035347A1 (en) * | 2015-08-25 | 2017-03-02 | The Broad Institute, Inc. | Screening of nucleic acid sequence variations via random loading of optically-encoded particles |
-
2017
- 2017-03-17 EP EP17305297.8A patent/EP3375889B1/en active Active
- 2017-03-17 LT LTEP17305297.8T patent/LT3375889T/en unknown
-
2018
- 2018-03-15 JP JP2019550678A patent/JP7050803B2/en active Active
- 2018-03-15 AU AU2018234084A patent/AU2018234084B2/en active Active
- 2018-03-15 CN CN201880018953.7A patent/CN110431239B/en active Active
- 2018-03-15 LT LTEPPCT/EP2018/056551T patent/LT3619319T/en unknown
- 2018-03-15 WO PCT/EP2018/056551 patent/WO2018167218A1/en active Application Filing
- 2018-03-15 US US16/494,537 patent/US11427853B2/en active Active
- 2018-03-15 CN CN202111005542.4A patent/CN114045328A/en active Pending
- 2018-03-15 EP EP18714983.6A patent/EP3619319B1/en active Active
- 2018-03-15 SG SG11201907569SA patent/SG11201907569SA/en unknown
- 2018-03-15 CA CA3055005A patent/CA3055005A1/en active Pending
-
2019
- 2019-09-08 IL IL26916819A patent/IL269168A/en unknown
-
2022
- 2022-08-26 US US17/896,470 patent/US20230063190A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3619319B1 (en) | 2021-06-23 |
| EP3619319A1 (en) | 2020-03-11 |
| US20230063190A1 (en) | 2023-03-02 |
| CA3055005A1 (en) | 2018-09-20 |
| US20200199649A1 (en) | 2020-06-25 |
| CN110431239A (en) | 2019-11-08 |
| CN110431239B (en) | 2021-07-09 |
| EP3375889B1 (en) | 2019-12-11 |
| WO2018167218A1 (en) | 2018-09-20 |
| IL269168A (en) | 2019-11-28 |
| LT3375889T (en) | 2020-06-25 |
| AU2018234084B2 (en) | 2024-06-13 |
| AU2018234084A1 (en) | 2019-09-26 |
| JP7050803B2 (en) | 2022-04-08 |
| CN114045328A (en) | 2022-02-15 |
| LT3619319T (en) | 2021-10-11 |
| US11427853B2 (en) | 2022-08-30 |
| EP3375889A1 (en) | 2018-09-19 |
| JP2020513807A (en) | 2020-05-21 |
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