SG11201907286SA - Production of fatty alcohols in rhodosporidium - Google Patents

Production of fatty alcohols in rhodosporidium

Info

Publication number
SG11201907286SA
SG11201907286SA SG11201907286SA SG11201907286SA SG11201907286SA SG 11201907286S A SG11201907286S A SG 11201907286SA SG 11201907286S A SG11201907286S A SG 11201907286SA SG 11201907286S A SG11201907286S A SG 11201907286SA SG 11201907286S A SG11201907286S A SG 11201907286SA
Authority
SG
Singapore
Prior art keywords
singapore
international
life sciences
limited
link
Prior art date
Application number
SG11201907286SA
Inventor
Lianghui Ji
Yanbin Liu
Chong Mei John Koh
Sihui Amy Yap
Si Te Ngoh
Original Assignee
Temasek Life Sciences Laboratory Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Temasek Life Sciences Laboratory Ltd filed Critical Temasek Life Sciences Laboratory Ltd
Publication of SG11201907286SA publication Critical patent/SG11201907286SA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01062—2-Acylglycerophosphocholine O-acyltransferase (2.3.1.62)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01042—Hexadecanal dehydrogenase (acylating) (1.2.1.42), i.e. fatty acyl-CoA reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01026—Sterol O-acyltransferase (2.3.1.26)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01158—Phospholipid:diacylglycerol acyltransferase (2.3.1.158)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

International Patent Classification: C12N 1/16 (2006.01) C12N 9/02 (2006.01) C12P 7/04 (2006.01) C12N 9/10 (2006.01) (21) International Application Number: PCT/SG2018/050045 (22) International Filing Date: 29 January 2018 (29.01.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/455,669 07 February 2017 (07.02.2017) US (71) Applicant: TEMASEK LIFE SCIENCES LABORATO- RY LIMITED [SG/SG]; National University of Singapore, 1 Research Link, Singapoore 117604 (SG). (72) Inventors: JI, Lianghui; c/o Temasek Life Sciences Lab- oratory Limited, National University of Singapore, 1 Re- search Link, Singapore 117604 (SG). LIU, Yanbin; c/o Temasek Life Sciences Laboratory Limited, National Uni- versity of Singapore, 1 Research Link, Singapore 117604 (SG). KOH, Chong Mei John; c/o Temasek Life Sciences Laboratory Limited, National University of Singapore, 1 Research Link, Singapore 117604 (SG). YAP, Sihui Amy; c/o Temasek Life Sciences Laboratory Limited, Nation- al University of Singapore, 1 Research Link, Singapore 117604 (SG). NGOH, Si Te; c/o Temasek Life Sciences Laboratory Limited, National University of Singapore, 1 Research Link, Singapore 117604 (SG). (74) Agent: DOWSING, Bruce John; Marks & Clerk Singa- pore LLP, Tanjong Pagar, P 0 Box 636, Singapore 910816 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) = (54) Title: PRODUCTION OF FATTY ALCOHOLS IN RHODOSPORIDIUM ( 3P 1.P A. FIG. MAI& (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 16 August 2018 (16.08.2018) WIP0 1 PCT onus onolomolomomomilionomorm# (10) International Publication Number WO 2018/147799 Al el (57) : The present invention relates to the field of fungal production fatty alcohols. More specifically, the present invention re- lates to genetically modified host cells, nucleic acid constructs and culture medium for the production of fatty alcohols in Rhodosporid- ium.
SG11201907286SA 2017-02-07 2018-01-29 Production of fatty alcohols in rhodosporidium SG11201907286SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762455669P 2017-02-07 2017-02-07
PCT/SG2018/050045 WO2018147799A1 (en) 2017-02-07 2018-01-29 Production of fatty alcohols in rhodosporidium

Publications (1)

Publication Number Publication Date
SG11201907286SA true SG11201907286SA (en) 2019-09-27

Family

ID=63107712

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201907286SA SG11201907286SA (en) 2017-02-07 2018-01-29 Production of fatty alcohols in rhodosporidium

Country Status (4)

Country Link
US (1) US11034981B2 (en)
EP (1) EP3580325A4 (en)
SG (1) SG11201907286SA (en)
WO (1) WO2018147799A1 (en)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103348008B (en) * 2010-12-23 2016-06-01 国际壳牌研究有限公司 Produce gene disruption of acyl CoA derivant
DK2718442T3 (en) * 2011-06-10 2017-08-14 Temasek Life Sciences Laboratory Ltd GENETIC MANIPULATION AND EXPRESSION SYSTEMS FOR SUBPHYLA OF PUCCINIOMYCOTINA AND USTILAGINOMYCOTINA
US10081821B2 (en) * 2014-09-08 2018-09-25 Temasek Life Sciences Laboratory Limited Methods for efficient production of polyunsaturated fatty acids (PUFA) in Rhodosporidium and Rhodotorula species
ES2579384B1 (en) * 2015-02-10 2017-07-07 Neol Biosolutions, S.A. Production of fatty alcohols
EP3277827B1 (en) * 2015-04-02 2021-03-24 Biopetrolia AB Fungal cells and methods for production of very long chain fatty acid derived products

Also Published As

Publication number Publication date
WO2018147799A1 (en) 2018-08-16
EP3580325A4 (en) 2021-04-28
US11034981B2 (en) 2021-06-15
EP3580325A1 (en) 2019-12-18
US20190360003A1 (en) 2019-11-28

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