SG11201903346QA - Gestational age assessment by methylation and size profiling of maternal plasma dna - Google Patents

Gestational age assessment by methylation and size profiling of maternal plasma dna

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Publication number
SG11201903346QA
SG11201903346QA SG11201903346QA SG11201903346QA SG11201903346QA SG 11201903346Q A SG11201903346Q A SG 11201903346QA SG 11201903346Q A SG11201903346Q A SG 11201903346QA SG 11201903346Q A SG11201903346Q A SG 11201903346QA SG 11201903346Q A SG11201903346Q A SG 11201903346QA
Authority
SG
Singapore
Prior art keywords
cell
international
free dna
gestational age
shatin
Prior art date
Application number
SG11201903346QA
Inventor
Yuk-Ming Dennis Lo
Rossa Wai Kwun Chiu
Kwan Chee Chan
Peiyong Jiang
Original Assignee
Univ Hong Kong Chinese
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Hong Kong Chinese filed Critical Univ Hong Kong Chinese
Publication of SG11201903346QA publication Critical patent/SG11201903346QA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) - (19) World Intellectual Property Organization - - 1111111111111111111111111111111111111111111111111111111111111111111111111110111111 ' International Bureau (10) International Publication Number (43) International Publication Date ......0\"\" WO 2018/072705 Al 26 April 2018 (26.04.2018) WIP0 I PCT (51) International Patent Classification: Yuen Road, Shatin, New Territories, Hong Kong 999077 C12Q 1/68 (2006.01) (CN). (21) International Application Number: (74) Agent: INSIGHT INTELLECTUAL PROPERTY PCT/CN2017/106663 LIMITED; 19 A, Tower A, InDo Building, No. 48A (22) International Filing Date: Zhichun Road, Haidian District, Beijing 100098 (CN). 18 October 2017 (18.10.2017) (81) Designated States (unless otherwise indicated, for every (25) Filing Language: English kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, (26) Publication Language: English CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (30) Priority Data: DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, 62/410,108 19 October 2016 (19.10.2016) US HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (71) Applicant: THE CHINESE UNIVERSITY OF HONG MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, KONG [CN/CN]; c/o Office of Research and Knowledge OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, Transfer Services, Room 301, Pi Ch'iu Building, Shatin, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, New Territories, Hong Kong 999077 (CN). TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (72) Inventors: LO, Yuk-Ming Dennis; 4th Floor, 7 King Tak (84) Designated States (unless otherwise indicated, for every Street, Homantin, Kowloon, Hong Kong 999077 (CN). kind of regional protection available): ARIPO (BW, GH, _ CHIU, Rossa Wai Kwun; House 31, Double Haven, 52 GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, Ma Lok Path, Shatin, New Territories, Hong Kong 999077 UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (CN). CHAN, Kwan Chee; Flat C, 15th Floor, Block 5, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, Peak One, Mei Tin Road, Shatin, New Territories, Hong EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, = Kong 999077 (CN). JIANG, Peiyong; Flat 7 on 1st Floor of MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, = _ Block B of Kwong Lam Court, Nos. 62-66 (No.62) Siu Lek = (54) Title: GESTATIONAL AGE ASSESSMENT BY METHYLATION AND SIZE PROFILING OF MATERNAL PLASMA DNA = (57) : Temporal variations in one or more characteristics measured from 800 a cell-free DNA sample are used to estimate a gestational age of a fetus. Exam- — 810 ple characteristics include the methylation level measured from the cell-free DNA sample, size of DNA fragments measured from the cell-free DNA sample (e.g., = Arialysits ere-free DNA isoleasks Pisa a Siuttple to determine whether the cell-free DNA molecule is proportion of fetal-derived DNA fragments longer than a specified size), and end- = = — Methylated At one mmotn Sites. ing patterns of the DNA fragments align to a reference genome. =8ID Determine arespectivertumber of cell-free DNA —_ oloteculea thar aremedaylated at each of the one ormore sites = = = — Calculate ameasund methylation level of cell-free DNA = Moleattlea in the sample = Obtain ate or more tatibtathin data pokis Shag speedy a gestational age corresponding to a calibration methylation level 11 850 in Compare the measured metbylation Level to a calibratima medtgladon level of at kart one calibration data points IN 11 © g6 1 N Estimate a gestational age of the fetus based owthe Ir----- comparison © 1-1 Fig. 8 0 N O [Continued on next page] WO 2018/072705 Al MIDEDIMOMMIDIREIHNIFIHMEIIMERVOIMIE TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))
SG11201903346QA 2016-10-19 2017-10-18 Gestational age assessment by methylation and size profiling of maternal plasma dna SG11201903346QA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662410108P 2016-10-19 2016-10-19
PCT/CN2017/106663 WO2018072705A1 (en) 2016-10-19 2017-10-18 Gestational age assessment by methylation and size profiling of maternal plasma dna

Publications (1)

Publication Number Publication Date
SG11201903346QA true SG11201903346QA (en) 2019-05-30

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US (2) US11512306B2 (en)
EP (2) EP3529377B1 (en)
CN (1) CN109890984B (en)
IL (1) IL265769B2 (en)
SG (1) SG11201903346QA (en)
WO (1) WO2018072705A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11479812B2 (en) 2015-05-11 2022-10-25 Natera, Inc. Methods and compositions for determining ploidy
CN108765371B (en) * 2018-04-25 2020-07-07 浙江大学 Segmentation method of unconventional cells in pathological section
CN109241628B (en) * 2018-09-08 2022-07-19 西北工业大学 Three-dimensional CAD model segmentation method based on graph theory and clustering
EP4065726A4 (en) * 2019-11-27 2024-04-03 Bioscreening And Diagnostics Llc Detecting congenital heart defects
CN115066504A (en) * 2020-02-05 2022-09-16 香港中文大学 Molecular analysis using long free fragments in pregnant women
CN114592074A (en) * 2022-04-12 2022-06-07 苏州市立医院 Target gene combination related to gestational age and application thereof
CN114898802B (en) * 2022-07-14 2022-09-30 臻和(北京)生物科技有限公司 Terminal sequence frequency distribution characteristic determination method, evaluation method and device based on plasma free DNA methylation sequencing data

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US8476013B2 (en) * 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
EA034241B1 (en) 2009-11-06 2020-01-21 Те Чайниз Юниверсити Ов Гонконг Method for performing prenatal diagnosis of a sequence imbalance
US8845539B2 (en) * 2010-12-22 2014-09-30 General Electric Company Methods and systems for estimating gestation age of a fetus
US9892230B2 (en) 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
FI3536807T3 (en) * 2012-09-20 2024-02-01 Univ Hong Kong Chinese Non-invasive determination of methylome of tumor from plasma
US9732390B2 (en) * 2012-09-20 2017-08-15 The Chinese University Of Hong Kong Non-invasive determination of methylome of fetus or tumor from plasma
KR20220127359A (en) 2014-07-25 2022-09-19 유니버시티 오브 워싱톤 Methods of determining tissues and/or cell types giving rise to cell-free dna, and methods of identifying a disease or disorder using same
US10319463B2 (en) 2015-01-23 2019-06-11 The Chinese University Of Hong Kong Combined size- and count-based analysis of maternal plasma for detection of fetal subchromosomal aberrations

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EP3529377B1 (en) 2023-04-05
EP3529377A1 (en) 2019-08-28
CN109890984B (en) 2024-03-29
US11512306B2 (en) 2022-11-29
IL265769A (en) 2019-06-30
WO2018072705A1 (en) 2018-04-26
IL265769B2 (en) 2023-12-01
EP4209598A1 (en) 2023-07-12
CN109890984A (en) 2019-06-14
US20180105807A1 (en) 2018-04-19
EP3529377A4 (en) 2020-05-27
US20230047963A1 (en) 2023-02-16
IL265769B1 (en) 2023-08-01

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