SG11201900669WA - Engineered imine reductases and methods for the reductive amination of ketone and amine compounds - Google Patents

Engineered imine reductases and methods for the reductive amination of ketone and amine compounds

Info

Publication number
SG11201900669WA
SG11201900669WA SG11201900669WA SG11201900669WA SG11201900669WA SG 11201900669W A SG11201900669W A SG 11201900669WA SG 11201900669W A SG11201900669W A SG 11201900669WA SG 11201900669W A SG11201900669W A SG 11201900669WA SG 11201900669W A SG11201900669W A SG 11201900669WA
Authority
SG
Singapore
Prior art keywords
california
redwood city
international
penobscot
drive
Prior art date
Application number
SG11201900669WA
Inventor
Margie Borra-Garske
Oscar Alvizo
Melissa Mayo
Stephan Jenne
Auric Sowell-Kantz
Carmela Molinaro
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of SG11201900669WA publication Critical patent/SG11201900669WA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y105/00—Oxidoreductases acting on the CH-NH group of donors (1.5)
    • C12Y105/01—Oxidoreductases acting on the CH-NH group of donors (1.5) with NAD+ or NADP+ as acceptor (1.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 01 March 2018 (01.03.2018) o I iolo 011111 OM REHM IMO 011 (10) International Publication Number WO 2018/038906 Al WIPO I PCT (51) International Patent Classification: CO7H 21/00 (2006.01) C12N 9/06 (2006.01) C07K 14/435 (2006.01) Cl 2N 15/52 (2006.01) C12N 9/00 (2006.01) C12N 15/53 (2006.01) (21) International Application Number: PCT/US2017/045838 (22) International Filing Date: 08 August 2017 (08.08.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/380,165 26 August 2016 (26.08.2016) US (71) Applicant: CODEXIS, INC. [US/US]; 200 Penobscot Dri- ve, Redwood City, California 94063 (US). (72) Inventors: BORRA-GARSKE, Margie, Tabuga; 200 Penobscot Drive, Redwood City, California 94063 (US). ALVIZO, Oscar; 200 Penobscot Drive, Redwood City, California 94063 (US). MAYO, Melissa, Ann; 200 Penob- scot Drive, Redwood City, California 94063 (US). JENNE, Stephan; 200 Penobscot Drive, Redwood City, California 94063 (US). SOWELL-KANTZ, Auric, Anthony; 200 Penobscot Drive, Redwood City, California 94063 (US). MOLINARO, Carmela; 126 East Lincoln Avenue, Rah- way, New Jersey 07065 (US). TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a)) (74) Agent: MACKNIGHT, Kamrin, T.; Codexis, Inc., 200 Penobscot Drive, Redwood City, California 94063 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, 1-1 TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, O GC Cr) O (54) Title: ENGINEERED MINE REDUCTASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND AMINE COMPOUNDS 0 (57) : The present application provides engineered polypeptides having imine or oxime reductase activity, polynucleotides N encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engi- C neered polypeptides with a range of ketone amine substrate compounds to prepare secondary and tertiary amine product compounds.
SG11201900669WA 2016-08-26 2017-08-08 Engineered imine reductases and methods for the reductive amination of ketone and amine compounds SG11201900669WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662380165P 2016-08-26 2016-08-26
PCT/US2017/045838 WO2018038906A1 (en) 2016-08-26 2017-08-08 Engineered imine reductases and methods for the reductive amination of ketone and amine compounds

Publications (1)

Publication Number Publication Date
SG11201900669WA true SG11201900669WA (en) 2019-03-28

Family

ID=61245145

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10202111589RA SG10202111589RA (en) 2016-08-26 2017-08-08 Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
SG11201900669WA SG11201900669WA (en) 2016-08-26 2017-08-08 Engineered imine reductases and methods for the reductive amination of ketone and amine compounds

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG10202111589RA SG10202111589RA (en) 2016-08-26 2017-08-08 Engineered imine reductases and methods for the reductive amination of ketone and amine compounds

Country Status (8)

Country Link
US (2) US11046936B2 (en)
EP (2) EP3515926B1 (en)
JP (2) JP7316662B2 (en)
CN (1) CN109863162B (en)
CA (1) CA3032519A1 (en)
IL (1) IL264686B1 (en)
SG (2) SG10202111589RA (en)
WO (1) WO2018038906A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018038906A1 (en) * 2016-08-26 2018-03-01 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
EP3963057A4 (en) * 2019-05-01 2023-06-14 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
CN110819601B (en) * 2019-11-04 2021-07-20 江南大学 Reductive amination enzyme, coding gene, recombinant vector, recombinant cell and application thereof
CN114317472B (en) * 2021-12-31 2022-07-05 长兴制药股份有限公司 High stereoselectivity imine reductase and preparation method and application thereof

Family Cites Families (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100356926B1 (en) 1993-09-07 2003-01-10 교와 핫꼬 고교 가부시끼가이샤 Process for producing trans-4-hydroxy-l-proline
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
BR9507817A (en) 1994-06-03 1997-09-16 Novo Nordisk Biotech Inc Construction of recombinant vector enzyme recombinant host cell laccase ascomycete or deuteromycete processes to obtain a laccase enzyme to improve the yield of recombinant enzyme to polymerize a lignin or lignosulfate substrate in solution to depolymerize the kraft paste to oxidize dyes or dye precursors to dye hair and to polymerize or oxidize a phenolic compound or aniline dye composition and container containing the same
WO1996000787A1 (en) 1994-06-30 1996-01-11 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
FI104465B (en) 1995-06-14 2000-02-15 Valio Oy Protein hydrolyzates for the treatment and prevention of allergies and their preparation and use
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
AU743305C (en) 1997-01-17 2006-03-30 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
EP1036198B1 (en) 1997-12-08 2012-09-26 California Institute Of Technology Method for creating polynucleotide and polypeptide sequences
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
JP4221100B2 (en) 1999-01-13 2009-02-12 エルピーダメモリ株式会社 Semiconductor device
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
EP1130093A1 (en) 1999-01-19 2001-09-05 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7702464B1 (en) 2001-08-21 2010-04-20 Maxygen, Inc. Method and apparatus for codon determining
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
EP1165757A1 (en) 1999-03-05 2002-01-02 Maxygen, Inc. Encryption of traits using split gene sequences
US6946458B2 (en) * 1999-07-22 2005-09-20 University Of South Florida N-thiolated beta-lactams: novel antibacterial agents for methicillin-resistant Staphylococcus aureus
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6519065B1 (en) 1999-11-05 2003-02-11 Jds Fitel Inc. Chromatic dispersion compensation device
EP1272967A2 (en) 2000-03-30 2003-01-08 Maxygen, Inc. In silico cross-over site selection
US6833447B1 (en) * 2000-07-10 2004-12-21 Monsanto Technology, Llc Myxococcus xanthus genome sequences and uses thereof
ES2564570T3 (en) 2002-03-01 2016-03-23 Codexis Mayflower Holdings, Llc Methods, systems and software for the identification of functional biomolecules
US7747391B2 (en) 2002-03-01 2010-06-29 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
US20050084907A1 (en) 2002-03-01 2005-04-21 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
JP4851687B2 (en) 2002-03-09 2012-01-11 マキシジェン， インコーポレイテッド Crossover optimization for directed evolution
EP1654354A1 (en) 2003-08-11 2006-05-10 Codexis, Inc. Improved ketoreductase polypeptides and related polynucleotides
US20090118130A1 (en) 2007-02-12 2009-05-07 Codexis, Inc. Structure-activity relationships
US8504498B2 (en) 2008-02-12 2013-08-06 Codexis, Inc. Method of generating an optimized, diverse population of variants
WO2009102901A1 (en) 2008-02-12 2009-08-20 Codexis, Inc. Method of generating an optimized, diverse population of variants
US8383346B2 (en) 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
US20090312196A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
CA2726850C (en) 2008-06-13 2015-06-02 Codexis, Inc. Method of synthesizing polynucleotide variants
EP2330190A1 (en) * 2008-09-01 2011-06-08 Daicel Chemical Industries, Ltd. Process for production of optically active amine derivative
CN102197141A (en) * 2008-09-01 2011-09-21 大赛璐化学工业株式会社 Process for production of optically active amine derivative
SG179197A1 (en) 2009-09-18 2012-04-27 Codexis Inc Reduced codon mutagenesis
JP2011177029A (en) * 2010-02-26 2011-09-15 Daicel Chemical Industries Ltd Method for producing optically-active amine derivative
EP2726651B1 (en) 2011-06-28 2018-11-07 Codexis, Inc. Protein variant generation by region shuffling
UY34585A (en) 2012-01-24 2013-09-02 Aicuris Gmbh & Co Kg B-LACTAMIC COMPOUNDS REPLACED WITH AMIDINE, ITS PREPARATION AND USE
US20150050658A1 (en) 2012-03-15 2015-02-19 Codexix, Inc. Gene shuffling methods
US9193957B2 (en) * 2012-05-11 2015-11-24 Codexis, Inc. Engineered imine reductases and methods for the reductive animation of ketone and amine compounds
LT2951579T (en) 2013-01-31 2024-05-27 Codexis, Inc. Methods, systems, and software for identifying bio-molecules using models of multiplicative form
ES2774965T3 (en) 2013-09-27 2020-07-23 Codexis Inc Predictive modeling based on structure
CN106232619B (en) * 2013-11-13 2022-09-09 科德克希思公司 Engineered imine reductases and methods for reductive amination of ketone and amine compounds
US9611163B2 (en) 2014-03-05 2017-04-04 Owens-Brockway Glass Container Inc. Process and apparatus for refining molten glass
US10370648B2 (en) * 2014-11-25 2019-08-06 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
WO2018038906A1 (en) * 2016-08-26 2018-03-01 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds

Also Published As

Publication number Publication date
EP3515926A1 (en) 2019-07-31
US11987818B2 (en) 2024-05-21
SG10202111589RA (en) 2021-11-29
WO2018038906A1 (en) 2018-03-01
CN109863162A (en) 2019-06-07
CN109863162B (en) 2023-03-17
CA3032519A1 (en) 2018-03-01
US20200208123A1 (en) 2020-07-02
JP2019526252A (en) 2019-09-19
EP3515926A4 (en) 2020-04-22
US11046936B2 (en) 2021-06-29
US20210284973A1 (en) 2021-09-16
EP3515926B1 (en) 2023-10-25
IL264686A (en) 2019-04-30
JP2022065092A (en) 2022-04-26
JP7316662B2 (en) 2023-07-28
IL264686B1 (en) 2024-03-01
EP4223766A3 (en) 2023-10-18
EP4223766A2 (en) 2023-08-09

Similar Documents

Publication Publication Date Title
SG11201908391XA (en) Methods for modulating an immune response
SG11201900746RA (en) Engineered antibodies and other fc-domain containing molecules with enhanced agonism and effector functions
SG11201909957TA (en) Engineered ligase variants
SG11201407417VA (en) Encoding and reconstruction of residual data based on support information
SG11201808709VA (en) T cell receptors
SG11201909955XA (en) Formulations of anti-lag3 antibodies and co-formulations of anti-lag3 antibodies and anti-pd-1 antibodies
SG11201807573VA (en) Methods for providing single-stranded rna
SG11201810522UA (en) Anti-cd40 antibodies and their uses
SG11201811431VA (en) Multispecific antibodies against cd40 and cd137
SG11201909777YA (en) Modulatory polynucleotides
SG11201900669WA (en) Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
SG11201906885TA (en) Methods for the administration of certain vmat2 inhibitors
SG11201906763RA (en) Pyrrolo [1,2-b] pyridazine derivatives
SG11201808750PA (en) T cell receptors
SG11201808990QA (en) Compositions for topical application of compounds
SG11201804132UA (en) Eif4-a-inhibiting compounds and methods related thereto
SG11201908326YA (en) Methods of treating neurodegenerative diseases
SG11201407669QA (en) Methods for improving safety of blood-brain barrier transport
SG11201805204WA (en) Nicotine particle capsule
SG11201807596YA (en) Gitr antibodies, methods, and uses
SG11201903012RA (en) Anti-c1s antibodies and methods of use thereof
SG11201808675VA (en) Method and kit for the generation of dna libraries for massively parallel sequencing
SG11201404836QA (en) Long-acting coagulation factors and methods of producing same
SG11201901438VA (en) Solid forms of cenicriviroc mesylate and processes of making solid forms of cenicriviroc mesylate
SG11201807949WA (en) Engineered immunoglobulins with altered fcrn binding