SG11201810864QA - Enzymatic glycosylation of steviol glycosides and other compounds with glucose-1-phosphate - Google Patents

Enzymatic glycosylation of steviol glycosides and other compounds with glucose-1-phosphate

Info

Publication number
SG11201810864QA
SG11201810864QA SG11201810864QA SG11201810864QA SG11201810864QA SG 11201810864Q A SG11201810864Q A SG 11201810864QA SG 11201810864Q A SG11201810864Q A SG 11201810864QA SG 11201810864Q A SG11201810864Q A SG 11201810864QA SG 11201810864Q A SG11201810864Q A SG 11201810864QA
Authority
SG
Singapore
Prior art keywords
international
penobscot
phosphate
redwood city
california
Prior art date
Application number
SG11201810864QA
Inventor
Jonathan Vroom
Stephanie Galanie
Oscar Alvizo
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of SG11201810864QA publication Critical patent/SG11201810864QA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01007—Sucrose phosphorylase (2.4.1.7)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

OHOH OH OH HO HO H PO 4 H 2 HO H30PO4 OH OH (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 21 December 2017 (21.12.2017) WIP0 I PCT IiiimmoimiolollmolommiololuiniolommovoimIE (10) International Publication Number WO 2017/218324 Al (51) International Patent Classification: C12N 9/10 (2006.01) C12N 15/54 (2006.01) C12N 15/52 (2006.01) Cl 2P 19/00 (2006.01) (21) International Application Number: PCT/US2017/036701 (22) International Filing Date: 09 June 2017 (09.06.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/350,450 15 June 2016 (15.06.2016) US (71) Applicant: CODEXIS, INC. [US/US]; 200 Penobscot Dri- ve, Redwood City, California 94063 (US). (72) Inventors: VROOM, Jonathan; 200 Penobscot Dri- ve, Redwood City, California 94063 (US). GALANIE, Stephanie, Sue; 200 Penobscot Drive, Redwood City, Cal- ifornia 94063 (US). ALVIZO, Oscar; 200 Penobscot Dri- ve, Redwood City, California 94063 (US). (74) Agent: MACKNIGHT, Kamrin, T.; Codexis, Inc., 200 Penobscot Drive, Redwood City, California 94063 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) (54) Title: ENZYMATIC GLYCOSYLATION OF STEVIOL GLYCOSIDES AND OTHER COMPOUNDS WITH GLU- COSE-1-PHOSPHATE a-Glucose-1-phosphate Stevioside Rebaudioside A Phosphate 1-1 © (57) : The present invention provides glycosyl transferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention C also provides methods of using these GT enzymes to generate products with 0-glucose linkages. FIG. 1
SG11201810864QA 2016-06-15 2017-06-09 Enzymatic glycosylation of steviol glycosides and other compounds with glucose-1-phosphate SG11201810864QA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662350450P 2016-06-15 2016-06-15
PCT/US2017/036701 WO2017218324A1 (en) 2016-06-15 2017-06-09 Enzymatic glycosylation of steviol glycosides and other compounds with glucose-1-phosphate

Publications (1)

Publication Number Publication Date
SG11201810864QA true SG11201810864QA (en) 2019-01-30

Family

ID=60664213

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201810864QA SG11201810864QA (en) 2016-06-15 2017-06-09 Enzymatic glycosylation of steviol glycosides and other compounds with glucose-1-phosphate

Country Status (14)

Country Link
US (1) US20190127772A1 (en)
EP (1) EP3472308A4 (en)
JP (1) JP2019517807A (en)
KR (1) KR20190017045A (en)
CN (1) CN109563493A (en)
AU (1) AU2017284103B2 (en)
BR (1) BR112018075816A2 (en)
CA (1) CA3027180A1 (en)
EA (1) EA201990034A1 (en)
IL (1) IL263596A (en)
MX (1) MX2018015517A (en)
PH (1) PH12018502655A1 (en)
SG (1) SG11201810864QA (en)
WO (1) WO2017218324A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020028039A1 (en) * 2018-07-30 2020-02-06 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
CA3142636A1 (en) * 2019-07-02 2021-01-07 Codexis, Inc. Engineered sucrose phosphorylase variant enzymes
KR102546737B1 (en) * 2020-11-25 2023-06-22 씨제이제일제당 주식회사 Composition comprising transglycosylated steviol glycoside
CN114317476B (en) * 2021-12-30 2023-07-14 南京诺云生物科技有限公司 Biocatalysis production process of glucosyl glycerine and sucrose phosphorylase thereof
CN114317477B (en) * 2021-12-30 2023-07-14 南京诺云生物科技有限公司 Sucrose phosphorylase and glucose-1-phosphoric acid production process
CN114231509B (en) * 2021-12-31 2023-07-25 南京诺云生物科技有限公司 Sucrose phosphorylase and glucosyl glycerol production process
CN114350737B (en) * 2022-01-14 2023-09-15 台州仙琚药业有限公司 Preparation method of 5 alpha, 6 alpha-epoxy-11 beta, 17 alpha-dihydroxypregna-3, 20-diethylene glycol ketal

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2815023B2 (en) * 1989-10-17 1998-10-27 農林水産省食品総合研究所長 Cellobiose production method
WO2009015268A2 (en) * 2007-07-24 2009-01-29 Wisconsin Alumni Research Foundation Engineered glycosyltransferases with expanded substrate specificity
US20130004979A1 (en) * 2010-06-11 2013-01-03 Wisconsin Alumni Research Foundation Glycosyltransferase reversibility for sugar nucleotide synthesis and microscale scanning
WO2013176738A1 (en) * 2012-05-22 2013-11-28 Purecircle Sdn Bhd High-purity steviol glycosides
JP6033632B2 (en) * 2012-10-15 2016-11-30 国立大学法人 新潟大学 Method for producing acidic β-glucosyl disaccharide using cellobionate phosphorylase
BR112015019160A2 (en) * 2013-02-11 2017-08-22 Dalgaard Mikkelsen Michael PRODUCTION OF STEVIOL GLYCOSIDES IN RECOMBINANT HOSTERS
KR101404728B1 (en) * 2013-02-28 2014-06-09 씨제이제일제당 (주) A method for preparation of Rebaudioside A from stevioside

Also Published As

Publication number Publication date
PH12018502655A1 (en) 2019-10-07
BR112018075816A2 (en) 2019-04-02
CA3027180A1 (en) 2017-12-21
EP3472308A4 (en) 2020-07-29
EA201990034A1 (en) 2019-06-28
WO2017218324A1 (en) 2017-12-21
IL263596A (en) 2019-02-03
CN109563493A (en) 2019-04-02
JP2019517807A (en) 2019-06-27
MX2018015517A (en) 2019-03-18
AU2017284103A1 (en) 2018-12-13
EP3472308A1 (en) 2019-04-24
US20190127772A1 (en) 2019-05-02
KR20190017045A (en) 2019-02-19
AU2017284103B2 (en) 2019-07-11

Similar Documents

Publication Publication Date Title
SG11201810864QA (en) Enzymatic glycosylation of steviol glycosides and other compounds with glucose-1-phosphate
SG11201906480QA (en) Engineered glycosyltransferases and steviol glycoside glucosylation methods
SG11201900930UA (en) Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides
SG11201811431VA (en) Multispecific antibodies against cd40 and cd137
SG11201810605UA (en) Selectively altering microbiota for immune modulation
SG11201909870SA (en) Compositions and methods of treating amyotrophic lateral sclerosis (als)
SG11201908680YA (en) Methods and compositions for preparing nucleic acid libraries
SG11201909949XA (en) Targeted immunotolerance
SG11201901206SA (en) Trimer stabilizing hiv envelope protein mutations
SG11201807252QA (en) Anti-lag-3 antibodies
SG11201803918VA (en) Production of steviol glycosides in recombinant hosts
SG11201809912UA (en) Hybrid carriers for nucleic acid cargo
SG11201803169WA (en) Cellulase variants and polynucleotides encoding same
SG11201804373VA (en) Compositions and methods for immunooncology
SG11201810777WA (en) Purification of multispecific antibodies
SG11201805120YA (en) Zika virus vaccine
SG11201804814YA (en) Materials and methods for delivering nucleic acids to cochlear and vestibular cells
SG11201803958WA (en) Nucleic acids encoding repetitive amino acid sequences rich in proline and alanine residues that have low repetitive nucleotide sequences
SG11201900400QA (en) Bispecific antibody-like binding proteins specifically binding to cd3 and cd123
SG11201808856TA (en) Production of steviol glycosides in recombinant hosts
SG11201809499UA (en) Processes for preparing phosphorodiamidate morpholino oligomers
SG11201809908TA (en) Stabilized glycopeptide antibiotic formulations
SG11201809483UA (en) Production of steviol glycosides in recombinant hosts
SG11201809594WA (en) Nicotine particles and compositions
SG11202000333UA (en) Bicyclic ketone compounds and methods of use thereof