SG11201806879SA - A simple one-step real-time molecular system for microrna detection - Google Patents
A simple one-step real-time molecular system for microrna detectionInfo
- Publication number
- SG11201806879SA SG11201806879SA SG11201806879SA SG11201806879SA SG11201806879SA SG 11201806879S A SG11201806879S A SG 11201806879SA SG 11201806879S A SG11201806879S A SG 11201806879SA SG 11201806879S A SG11201806879S A SG 11201806879SA SG 11201806879S A SG11201806879S A SG 11201806879SA
- Authority
- SG
- Singapore
- Prior art keywords
- nucleic acid
- singapore
- international
- acid sequence
- target nucleic
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization M1111101110101011111 HO 11111 01101011111011011101100 111110 ill OEN 11 International Bureau .. .... ..Yejd (10) International Publication Number (43) ..... .....1 International Publication Date WO 2017/146653 Al 31 August 2017 (31.08.2017) WIPO I PCT (51) International Patent Classification: Molecular and Cell Biology, 61 Biopolis Drive, Proteos, C12Q 1/68 (2006.01) Singapore 138673 (SG). TANG, Melissa Xue Mei; c/o In- (21) International Application Number: stitute of Molecular and Cell Biology, 61 Biopolis Drive, PCT/SG2017/050086 Proteos, Singapore 138673 (SG). (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; (22) International Filing Date: P.O. Box 1531, Robinson Road Post Office, Singapore 24 February 2017 (24.02.2017) 903031 (SG). (25) Filing Language: English (81) Designated States (unless otherwise indicated, for every (26) Publication Language: English kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (30) Priority Data: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, 10201601384T 24 February 2016 (24.02.2016) SG DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, Connexis North Tower, Singapore 138632 (SG). MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, (72) Inventors: AW, Sherry; c/o Institute of Molecular and RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, Cell Biology, 61 Biopolis Drive, Proteos, Singapore TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, 138673 (SG). TEO, Yin Nah; c/o Institute of Molecular ZA, ZM, ZW. and Cell Biology, 61 Biopolis Drive, Proteos, Singapore _ 138673 (SG). COHEN, Stephen Michael; c/o Institute of (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, [Continued on next page] = = (54) Title: A SIMPLE ONE-STEP REAL-TIME MOLECULAR SYSTEM FOR MICRORNA DETECTION o 0 (57) : The present application discloses an isolated nucleic acid mo- lecule for detecting the presence of a target nucleic acid sequence and uses t• thereof, wherein said molecule is capable of forming bimolecular interactions = Niev,, with a target nucleic acid sequence and forms a ternary complex stabilising a detection agent when bound. The detection agent is preferably DFHBI ((Z)-4- Variable (3,5-difluoro-4-hydroxybenzylidene)-1,2-dimethy1-1 H-imidazol-5(4H)-one. = Variable Stem-loop P4 The target nucleic acid is preferably a miRNA sequence. Preferably, the isol- = Stem-loop ,-)3 ated nucleic acid sequence comprises two domains that complementarily bind to the 5' and 3' ends of the target nucleic acid sequence. The isolated nucleic acid sequence also comprises regions of nucleic acids that do not bind with the target nucleic acid sequence and are capable of forming secondary struc- = — = .. tures, such as stems, stem loops, t-RNA type structures, cloverleaves, tetra- loops and pseudoknots. The application further discloses corresponding meth- ods of determining a disease in a subject by using the isolated nucleic acid se - quence to detect one or more disease-specific target nucleic acids. = = — G G - C Stem P2 u'll 9 — € 3 —c. - . _ 1-1 Stem- en ir) loop P1 ,tD ,tD 41 G G 7r 11 --.... Figure 8 IN Il © ei O WO 2017/146653 Al MIDEDIMOMOIDEIR010130101fillEINUMEMOIMIE GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, Declarations under Rule 4.17: TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, DE, LT, of inventorship (Rule 4.17w)) Published: LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). SE, GA, with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a))
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SG10201601384T | 2016-02-24 | ||
PCT/SG2017/050086 WO2017146653A1 (en) | 2016-02-24 | 2017-02-24 | A simple one-step real-time molecular system for microrna detection |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11201806879SA true SG11201806879SA (en) | 2018-09-27 |
Family
ID=59685435
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201806879SA SG11201806879SA (en) | 2016-02-24 | 2017-02-24 | A simple one-step real-time molecular system for microrna detection |
SG10201913287QA SG10201913287QA (en) | 2016-02-24 | 2017-02-24 | A simple one-step real-time molecular system for microrna detection |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG10201913287QA SG10201913287QA (en) | 2016-02-24 | 2017-02-24 | A simple one-step real-time molecular system for microrna detection |
Country Status (3)
Country | Link |
---|---|
US (1) | US11174505B2 (en) |
SG (2) | SG11201806879SA (en) |
WO (1) | WO2017146653A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SG11202111264TA (en) * | 2019-04-12 | 2021-11-29 | Agency Science Tech & Res | A ribozyme comprising a target-binding domain |
CN115807079A (en) * | 2021-09-14 | 2023-03-17 | 四川大学 | Kit for detecting peripheral blood microRNA |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5925517A (en) * | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
US11453646B2 (en) * | 2011-07-27 | 2022-09-27 | Cornell University | Methods for RNA detection and quantification |
US9664676B2 (en) | 2013-09-06 | 2017-05-30 | Cornell University | RNA sequences that induce fluorescence of small molecule fluorophores |
-
2017
- 2017-02-24 SG SG11201806879SA patent/SG11201806879SA/en unknown
- 2017-02-24 SG SG10201913287QA patent/SG10201913287QA/en unknown
- 2017-02-24 US US16/079,992 patent/US11174505B2/en active Active
- 2017-02-24 WO PCT/SG2017/050086 patent/WO2017146653A1/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2017146653A1 (en) | 2017-08-31 |
US20190382830A1 (en) | 2019-12-19 |
US11174505B2 (en) | 2021-11-16 |
SG10201913287QA (en) | 2020-02-27 |
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