SG11201806623VA - Bacteria over-expressing c-di-amp and therapeutic methods - Google Patents

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2016/130616 Al 18 August 2016 (18.08.2016) WIPO I PCT 11111111111111 0 11101 010 11111 0111 11111 01110111 01111101E11011 111111111111111 0111111 (51) International Patent Classification: A61K 39/04 (2006.01) A61K 48/00 (2006.01) A61K 35/74 (2006.01) A61P 35/00 (2006.01) (21) International Application Number: PCT/US2016/017248 (22) International Filing Date: 10 February 2016 (10.02.2016) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/114,610 11 February 2015 (11.02.2015) US (71) Applicant: THE JOHNS HOPKINS UNIVERSITY [US/US]; 3400 N. Charles Street, Baltimore, Maryland 21218 (US). (72) Inventors: BISHAI, William R.; 1202 Ridervale Road, Baltimore, Maryland 21204 (US). DEY, Ruchi Jain; 6316 Greenspring Ave., Apt. 208, Baltimore, Maryland 21209 (US). DEY, Bappaditya; 6316 Greenspring Ave., Apt. 208, Baltimore, Maryland 21209 (US). (74) Agent: FEULNER, Gregory; 100 N. Charles Street, 5th Floor, Baltimore, Maryland 21201 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) W O 20 16/ 1306 16 Al (54) Title: BACTERIA OVER-EXPRESSING C-DI-AMP AND THERAPEUTIC METHODS (57) : The present invention includes the discovery of a strain of Mycobacterium comprising an expression vector encoding a di-adenylate cyclase enzyme. The Mycobacterium is selected from the group consisting of Mycobacterium tuberculosis, Mycobac- terium bovis, or a combination thereof and the preferred strain of Mycobacterium is BCG. The preferred expression vector is a my - cobacterial expression vector including an hsp60 promoter and a DNA sequence of diadenylate cyclase (disA), or a functional part thereof. The strains of Mycobacterium are used in therapeutic applications including tuberculosis and cancer.?