SG11201707909YA - Method for detecting genomic variations using circularised mate-pair library and shotgun sequencing - Google Patents

Method for detecting genomic variations using circularised mate-pair library and shotgun sequencing

Info

Publication number
SG11201707909YA
SG11201707909YA SG11201707909YA SG11201707909YA SG11201707909YA SG 11201707909Y A SG11201707909Y A SG 11201707909YA SG 11201707909Y A SG11201707909Y A SG 11201707909YA SG 11201707909Y A SG11201707909Y A SG 11201707909YA SG 11201707909Y A SG11201707909Y A SG 11201707909YA
Authority
SG
Singapore
Prior art keywords
circularised
mate
shotgun sequencing
pair library
genomic variations
Prior art date
Application number
SG11201707909YA
Other languages
English (en)
Inventor
Yijun Ruan
Original Assignee
Jackson Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jackson Lab filed Critical Jackson Lab
Publication of SG11201707909YA publication Critical patent/SG11201707909YA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/301—Sonication
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/303—Applying a physical force on a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11201707909YA 2015-04-02 2016-04-01 Method for detecting genomic variations using circularised mate-pair library and shotgun sequencing SG11201707909YA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562142088P 2015-04-02 2015-04-02
PCT/US2016/025475 WO2016161236A1 (fr) 2015-04-02 2016-04-01 Procédé pour détecter des variations génomiques à l'aide de banques de paires d'appariement circularisées et d'un séquençage aléatoire

Publications (1)

Publication Number Publication Date
SG11201707909YA true SG11201707909YA (en) 2017-10-30

Family

ID=55795182

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201707909YA SG11201707909YA (en) 2015-04-02 2016-04-01 Method for detecting genomic variations using circularised mate-pair library and shotgun sequencing
SG10201909182W SG10201909182WA (en) 2015-04-02 2016-04-01 Method for detecting genomic variations using circularised mate-pair library and shotgun sequencing

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG10201909182W SG10201909182WA (en) 2015-04-02 2016-04-01 Method for detecting genomic variations using circularised mate-pair library and shotgun sequencing

Country Status (11)

Country Link
US (1) US20180135120A1 (fr)
EP (1) EP3277840A1 (fr)
JP (1) JP2018509928A (fr)
KR (1) KR20170133420A (fr)
CN (1) CN107889508A (fr)
AU (1) AU2016242953A1 (fr)
CA (1) CA2980769A1 (fr)
HK (1) HK1250171A1 (fr)
IL (1) IL254713A0 (fr)
SG (2) SG11201707909YA (fr)
WO (1) WO2016161236A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111304287B (zh) * 2018-12-13 2020-09-15 北京先声医学检验实验室有限公司 基于纳米孔测序平台的痰液样本建库方法、鉴定方法及试剂盒
JP6883600B2 (ja) * 2019-03-07 2021-06-09 シスメックス株式会社 医療関係者による遺伝子情報の解釈を支援する方法、情報管理システム、統合データ管理装置
CN110349635B (zh) * 2019-06-11 2021-06-11 华南理工大学 一种基因测序数据质量分数的并行压缩方法
CN113724788B (zh) * 2021-07-29 2023-09-12 哈尔滨医科大学 一种鉴定肿瘤细胞的染色体外环状dna组成基因的方法
US20230121442A1 (en) 2021-10-06 2023-04-20 Johnson & Johnson Consumer Inc. Method of Quantifying Product Impact on Human Microbiome
CN114438182B (zh) * 2022-02-18 2024-04-05 杭州柏熠科技有限公司 基于纳米孔测序的进境植物检疫性病毒鉴定方法及应用
CN114743598B (zh) * 2022-06-14 2022-09-02 湖南大学 一种基于信息论检测新冠病毒谱系间重组的方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2159285B1 (fr) 2003-01-29 2012-09-26 454 Life Sciences Corporation Procédés d'amplification et de séquençage d'acides nucléiques
CA2712426A1 (fr) * 2008-02-05 2009-08-13 F. Hoffmann-La Roche Ag Sequencage d'extremites par paire
US8383345B2 (en) * 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
CA2821299C (fr) * 2010-11-05 2019-02-12 Frank J. Steemers Liaison entre des lectures de sequences a l'aide de codes marqueurs apparies
US10457936B2 (en) * 2011-02-02 2019-10-29 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
WO2014142850A1 (fr) * 2013-03-13 2014-09-18 Illumina, Inc. Procédés et compositions pour le séquençage d'acide nucléique

Also Published As

Publication number Publication date
KR20170133420A (ko) 2017-12-05
CN107889508A (zh) 2018-04-06
AU2016242953A1 (en) 2017-10-12
HK1250171A1 (zh) 2018-11-30
EP3277840A1 (fr) 2018-02-07
CA2980769A1 (fr) 2016-10-06
US20180135120A1 (en) 2018-05-17
SG10201909182WA (en) 2019-11-28
IL254713A0 (en) 2017-11-30
JP2018509928A (ja) 2018-04-12
WO2016161236A1 (fr) 2016-10-06

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