SG11201605088WA - Method for testing mutant gene through real-time polymerase chain reaction using dna polymerase with inhibited activity of 5'-flap endonuclease - Google Patents

Method for testing mutant gene through real-time polymerase chain reaction using dna polymerase with inhibited activity of 5'-flap endonuclease

Info

Publication number
SG11201605088WA
SG11201605088WA SG11201605088WA SG11201605088WA SG11201605088WA SG 11201605088W A SG11201605088W A SG 11201605088WA SG 11201605088W A SG11201605088W A SG 11201605088WA SG 11201605088W A SG11201605088W A SG 11201605088WA SG 11201605088W A SG11201605088W A SG 11201605088WA
Authority
SG
Singapore
Prior art keywords
real
chain reaction
mutant gene
flap endonuclease
dna polymerase
Prior art date
Application number
SG11201605088WA
Inventor
Jae Jong Kim
Sun Ho Cha
Si Kyu Lim
Original Assignee
Genotech Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genotech Corp filed Critical Genotech Corp
Publication of SG11201605088WA publication Critical patent/SG11201605088WA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/307—Single strand endonuclease

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
SG11201605088WA 2014-01-08 2015-01-08 Method for testing mutant gene through real-time polymerase chain reaction using dna polymerase with inhibited activity of 5'-flap endonuclease SG11201605088WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020140002306A KR101598398B1 (en) 2014-01-08 2014-01-08 Mutant Detection Method by Real-Time Polymerase Chain Reaction using selective working of 5'-flap endonuclease activity
PCT/KR2015/000167 WO2015105336A1 (en) 2014-01-08 2015-01-08 Method for testing mutant gene through real-time polymerase chain reaction using dna polymerase with inhibited activity of 5'-flap endonuclease

Publications (1)

Publication Number Publication Date
SG11201605088WA true SG11201605088WA (en) 2016-07-28

Family

ID=53524113

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201605088WA SG11201605088WA (en) 2014-01-08 2015-01-08 Method for testing mutant gene through real-time polymerase chain reaction using dna polymerase with inhibited activity of 5'-flap endonuclease

Country Status (12)

Country Link
US (2) US10337052B2 (en)
EP (1) EP3093349B1 (en)
JP (1) JP6312277B2 (en)
KR (1) KR101598398B1 (en)
CN (1) CN106068329B (en)
AU (1) AU2015205143B2 (en)
BR (1) BR112016015895A2 (en)
CA (1) CA2936063C (en)
MX (1) MX2016009008A (en)
RU (1) RU2016126512A (en)
SG (1) SG11201605088WA (en)
WO (1) WO2015105336A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108531561A (en) * 2017-03-01 2018-09-14 云南序源生物技术开发有限公司 A kind of quick detection is used to identify the kit and method in the SNP features site of Y chromosome haplotype pedigree
WO2019168261A1 (en) * 2018-02-28 2019-09-06 (주)제노텍 Method for analyzing qualitative or quantitative mutant genotype and real-time pcr kit for performing same method
CN110408679B (en) * 2019-07-30 2022-12-16 江西师范大学 Electrochemical acute leukemia gene Pax-5a detection method based on enzyme-assisted circulation signal amplification

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60125243T2 (en) * 2000-07-03 2007-05-31 Applera Corp., Foster City POLYNUCLEOTIDE SEQUENCE ASSAY
EP1540003A4 (en) * 2002-09-19 2006-06-21 Applera Corp Methods and composition for detecting targets
AU2003273778A1 (en) * 2002-10-21 2004-05-04 Exiqon A/S Oligonucleotide analogues for detecting and analyzing nucleic acids
WO2005092038A2 (en) * 2004-03-22 2005-10-06 The Johns Hopkins University Methods for the detection of nucleic acid differences
US7846704B2 (en) * 2004-07-23 2010-12-07 National Institute Of Advanced Industrial Science And Technology Flap endonuclease mutants
JP2008067649A (en) * 2006-09-14 2008-03-27 Osaka Univ Kit for diagnosing fecundity, method for detecting mutation, polynucleotide and polypeptide
AU2009225835B2 (en) * 2008-03-15 2013-02-14 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules during amplification reactions
CN101440407A (en) * 2008-12-12 2009-05-27 深圳华大基因科技有限公司 Method for detecting nucleotide mutant site
CN101671674B (en) * 2009-03-27 2010-09-22 郑立谋 Annular primer for amplification of nucleic acid and application thereof
US8940487B2 (en) * 2010-02-09 2015-01-27 UmiTaq Bio Methods and compositions for universal detection of nucleic acids
KR101164172B1 (en) * 2010-02-12 2012-07-11 중앙대학교 산학협력단 Method for Detecting Amantadine Resistant Influenza A Virus Using Real-Time Polymerase Chain Reaction
KR20120046018A (en) * 2010-10-04 2012-05-09 삼성테크윈 주식회사 Real time pcr detection of single nucleotide polymorphisms
CN102140509B (en) * 2010-12-28 2014-05-28 中国人民解放军第四军医大学 Gene mutation detection method based on nucleic acid amplification on solid carrier
KR20130091939A (en) * 2012-02-09 2013-08-20 주식회사 씨젠 Methods for detecting nucleotide variation

Also Published As

Publication number Publication date
CA2936063C (en) 2019-02-19
JP2017501735A (en) 2017-01-19
EP3093349A1 (en) 2016-11-16
US20190316188A1 (en) 2019-10-17
EP3093349B1 (en) 2019-02-20
US10337052B2 (en) 2019-07-02
RU2016126512A3 (en) 2019-02-19
JP6312277B2 (en) 2018-04-18
CN106068329A (en) 2016-11-02
MX2016009008A (en) 2017-06-06
AU2015205143A1 (en) 2016-07-21
KR20150082848A (en) 2015-07-16
EP3093349A4 (en) 2017-09-06
BR112016015895A2 (en) 2017-09-19
WO2015105336A1 (en) 2015-07-16
US20160326577A1 (en) 2016-11-10
KR101598398B1 (en) 2016-02-29
CN106068329B (en) 2020-03-27
RU2016126512A (en) 2018-12-06
CA2936063A1 (en) 2015-07-16
AU2015205143B2 (en) 2017-12-14

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