SG11201601890UA - Application of oligo-dt molecules to avoid generation of high molecular pcr products induced by polya-carrier - Google Patents

Application of oligo-dt molecules to avoid generation of high molecular pcr products induced by polya-carrier

Info

Publication number
SG11201601890UA
SG11201601890UA SG11201601890UA SG11201601890UA SG11201601890UA SG 11201601890U A SG11201601890U A SG 11201601890UA SG 11201601890U A SG11201601890U A SG 11201601890UA SG 11201601890U A SG11201601890U A SG 11201601890UA SG 11201601890U A SG11201601890U A SG 11201601890UA
Authority
SG
Singapore
Prior art keywords
polya
oligo
molecules
carrier
application
Prior art date
Application number
SG11201601890UA
Other languages
English (en)
Inventor
Frank Bergmann
Stefanie Froehner
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of SG11201601890UA publication Critical patent/SG11201601890UA/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11201601890UA 2013-09-13 2014-09-10 Application of oligo-dt molecules to avoid generation of high molecular pcr products induced by polya-carrier SG11201601890UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13184307 2013-09-13
PCT/EP2014/069294 WO2015036434A1 (en) 2013-09-13 2014-09-10 Application of oligo-dt molecules to avoid generation of high molecular pcr products induced by polya-carrier

Publications (1)

Publication Number Publication Date
SG11201601890UA true SG11201601890UA (en) 2016-04-28

Family

ID=49165596

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201601890UA SG11201601890UA (en) 2013-09-13 2014-09-10 Application of oligo-dt molecules to avoid generation of high molecular pcr products induced by polya-carrier

Country Status (9)

Country Link
US (1) US9611505B2 (es)
EP (1) EP3044326B1 (es)
JP (1) JP6556728B2 (es)
CN (1) CN105531381B (es)
CA (1) CA2923083C (es)
ES (1) ES2657988T3 (es)
HK (1) HK1219301A1 (es)
SG (1) SG11201601890UA (es)
WO (1) WO2015036434A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2912197B1 (en) 2012-10-24 2019-08-07 Takara Bio USA, Inc. Template switch-based methods for producing a product nucleic acid
EP4389888A3 (en) * 2013-10-17 2024-09-04 Takara Bio USA, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
US9719136B2 (en) 2013-12-17 2017-08-01 Takara Bio Usa, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
GB201805676D0 (en) * 2018-04-05 2018-05-23 Imperial Innovations Ltd Compositions

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0710296A4 (en) * 1993-07-23 1999-05-26 Bio Rad Laboratories LINEAR AMPLIFICATION OF NUCLEIC ACIDS
ATE308554T1 (de) * 1994-07-07 2005-11-15 Geron Corp Säugetier-telomerase
NZ337445A (en) * 1997-02-26 2000-12-22 Gen Hospital Corp Transgenic animals and cell lines for screening drugs effective for the treatment or prevention of alzheimer's disease
US7683035B1 (en) * 1999-02-23 2010-03-23 Qiagen, Gmbh Method of stabilizing and/or isolating nucleic acids
WO2005012499A2 (en) * 2003-08-01 2005-02-10 Integrated Dna Technologies, Inc. Methods for amplifying polymeric nucleic acids
GB0818609D0 (en) * 2008-10-10 2008-11-19 Univ Hull apparatus and method
US8618253B2 (en) * 2010-05-25 2013-12-31 Samsung Techwin Co., Ltd. Modified RNAse H and detection of nucleic acid amplification
WO2012054975A1 (en) * 2010-10-28 2012-05-03 Clinical Genomics Pty. Ltd. Method of microvesicle enrichment
KR101870311B1 (ko) * 2012-03-09 2018-06-25 (주)바이오니아 핫스타트 역전사반응 또는 핫스타트 역전사 중합효소 연쇄반응용 조성물
AU2013232131B2 (en) * 2012-03-13 2018-09-20 Integrated Dna Technologies, Inc. Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase

Also Published As

Publication number Publication date
HK1219301A1 (zh) 2017-03-31
ES2657988T3 (es) 2018-03-07
CA2923083C (en) 2018-07-17
EP3044326B1 (en) 2017-12-06
US20150079600A1 (en) 2015-03-19
US9611505B2 (en) 2017-04-04
CN105531381A (zh) 2016-04-27
WO2015036434A1 (en) 2015-03-19
JP6556728B2 (ja) 2019-08-07
CA2923083A1 (en) 2015-03-19
EP3044326A1 (en) 2016-07-20
CN105531381B (zh) 2019-08-09
JP2016529914A (ja) 2016-09-29

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