SG10201610621XA - An Expression Construct For Sensing Cell Density And Substrate Availability And Its Use In Conversion Of Hydroxycinnamic Acids - Google Patents

An Expression Construct For Sensing Cell Density And Substrate Availability And Its Use In Conversion Of Hydroxycinnamic Acids

Info

Publication number
SG10201610621XA
SG10201610621XA SG10201610621XA SG10201610621XA SG10201610621XA SG 10201610621X A SG10201610621X A SG 10201610621XA SG 10201610621X A SG10201610621X A SG 10201610621XA SG 10201610621X A SG10201610621X A SG 10201610621XA SG 10201610621X A SG10201610621X A SG 10201610621XA
Authority
SG
Singapore
Prior art keywords
conversion
cell density
expression construct
sensing cell
hydroxycinnamic acids
Prior art date
Application number
SG10201610621XA
Other languages
English (en)
Inventor
Matthew Wook Chang
Tat Ming Samuel Lo
Chueh Loo Poh
Original Assignee
Univ Nanyang Tech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Nanyang Tech filed Critical Univ Nanyang Tech
Publication of SG10201610621XA publication Critical patent/SG10201610621XA/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/62Carboxylic acid esters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93Ligases (6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y402/00Carbon-oxygen lyases (4.2)
    • C12Y402/01Hydro-lyases (4.2.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y602/00Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01Acid-Thiol Ligases (6.2.1)
    • C12Y602/010124-Coumarate-CoA ligase (6.2.1.12)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG10201610621XA 2012-06-19 2013-06-12 An Expression Construct For Sensing Cell Density And Substrate Availability And Its Use In Conversion Of Hydroxycinnamic Acids SG10201610621XA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261661593P 2012-06-19 2012-06-19

Publications (1)

Publication Number Publication Date
SG10201610621XA true SG10201610621XA (en) 2017-02-27

Family

ID=49769121

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201610621XA SG10201610621XA (en) 2012-06-19 2013-06-12 An Expression Construct For Sensing Cell Density And Substrate Availability And Its Use In Conversion Of Hydroxycinnamic Acids
SG11201408353QA SG11201408353QA (en) 2012-06-19 2013-06-12 An expression construct for sensing cell density and substrate availability and its use in conversion of hydroxycinnamic acids

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201408353QA SG11201408353QA (en) 2012-06-19 2013-06-12 An expression construct for sensing cell density and substrate availability and its use in conversion of hydroxycinnamic acids

Country Status (4)

Country Link
US (1) US10683521B2 (fr)
EP (1) EP2877582B1 (fr)
SG (2) SG10201610621XA (fr)
WO (1) WO2013191651A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201703528YA (en) * 2014-11-03 2017-05-30 Univ Nanyang Tech A recombinant expression system that senses pathogenic microorganisms
CN110951760B (zh) * 2019-12-20 2021-09-03 台州职业技术学院 一种蛋白延时表达开关及其在葡萄糖二酸生产中应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7112721B2 (en) * 2000-02-08 2006-09-26 Sakata Seed Corporation Methods and constructs for plant transformation
AU2005261862B2 (en) * 2004-07-15 2010-08-26 Dsm Ip Assets B.V. Biochemical synthesis of 1,4-butanediamine
WO2007061631A2 (fr) * 2005-11-15 2007-05-31 Glycofi, Inc Production de glycoproteines a o-glycosylation reduite
IN2014DN10111A (fr) * 2012-06-19 2015-08-21 Dsm Ip Assets Bv
US9439972B2 (en) * 2012-09-10 2016-09-13 Ad Lunam Labs, Inc. Antifungal serum
US10077475B2 (en) * 2013-01-24 2018-09-18 California Institute Of Technology FRET-based analytes detection and related methods and systems

Also Published As

Publication number Publication date
EP2877582B1 (fr) 2018-12-19
WO2013191651A1 (fr) 2013-12-27
US10683521B2 (en) 2020-06-16
US20150176039A1 (en) 2015-06-25
EP2877582A1 (fr) 2015-06-03
EP2877582A4 (fr) 2016-03-09
SG11201408353QA (en) 2015-01-29

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