SE8505568D0 - EXPRESSION VECTORS AND METHOD FOR THEIR PRODUCTION - Google Patents
EXPRESSION VECTORS AND METHOD FOR THEIR PRODUCTIONInfo
- Publication number
- SE8505568D0 SE8505568D0 SE8505568A SE8505568A SE8505568D0 SE 8505568 D0 SE8505568 D0 SE 8505568D0 SE 8505568 A SE8505568 A SE 8505568A SE 8505568 A SE8505568 A SE 8505568A SE 8505568 D0 SE8505568 D0 SE 8505568D0
- Authority
- SE
- Sweden
- Prior art keywords
- operon
- hybrid
- coding
- rrna
- shortened
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Endocrinology (AREA)
- Diabetes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
An expression vector comprising a hybrid operon which combines parts of an E.coli rRNA operon and a part of a protein coding operon, which may be constructed by removing most of the structural part of an E.coli rRNA operon carried by a suitable plasmid to obtain a shortened rRNA operon, combining said shortened rRNA operon with a part of a protein-coding operon to produce an effective hybrid expression control region and finally inserting a DNA fragment coding for a desired polypeptide into the combined operon to ensure gene expression under the control of said hybrid expression control region.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
HU844363A HU194309B (en) | 1984-11-26 | 1984-11-26 | Process for producing expressive vectors |
Publications (2)
Publication Number | Publication Date |
---|---|
SE8505568D0 true SE8505568D0 (en) | 1985-11-25 |
SE8505568L SE8505568L (en) | 1986-05-27 |
Family
ID=10967756
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SE8505568A SE8505568L (en) | 1984-11-26 | 1985-11-25 | EXPRESSION VECTOR AND METHOD OF PRODUCING THEREOF |
Country Status (15)
Country | Link |
---|---|
JP (1) | JPS61166395A (en) |
AU (1) | AU5034785A (en) |
BE (1) | BE903695A (en) |
DE (1) | DE3541783A1 (en) |
DK (1) | DK547285A (en) |
FI (1) | FI854655A (en) |
FR (1) | FR2573773A1 (en) |
GB (1) | GB2167760A (en) |
HU (1) | HU194309B (en) |
IL (1) | IL77152A0 (en) |
IT (1) | IT1191642B (en) |
NL (1) | NL8503257A (en) |
PL (1) | PL256453A1 (en) |
SE (1) | SE8505568L (en) |
ZA (1) | ZA859033B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2592541Y2 (en) * | 1991-02-15 | 1999-03-24 | 愛知機械工業株式会社 | Lubrication structure of manual transmission |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5756495A (en) * | 1980-09-24 | 1982-04-05 | Kyowa Hakko Kogyo Co Ltd | Novel dna-introduction vector and recombinant dna |
IL65775A (en) * | 1981-05-18 | 1985-05-31 | Genentech Inc | Microbial hybrid promotors and plasmids and e.coli comprising them |
GB2135678B (en) * | 1983-02-17 | 1986-08-28 | Univ New York State Res Found | Ribosomal rna operon and method of incorporating same in plasmids and bacteria |
-
1984
- 1984-11-26 HU HU844363A patent/HU194309B/en not_active IP Right Cessation
-
1985
- 1985-11-22 AU AU50347/85A patent/AU5034785A/en not_active Abandoned
- 1985-11-22 BE BE1/011380A patent/BE903695A/en not_active IP Right Cessation
- 1985-11-25 SE SE8505568A patent/SE8505568L/en not_active Application Discontinuation
- 1985-11-26 PL PL25645385A patent/PL256453A1/en unknown
- 1985-11-26 DE DE19853541783 patent/DE3541783A1/en not_active Withdrawn
- 1985-11-26 IT IT22982/85A patent/IT1191642B/en active
- 1985-11-26 DK DK547285A patent/DK547285A/en not_active Application Discontinuation
- 1985-11-26 GB GB08529127A patent/GB2167760A/en not_active Withdrawn
- 1985-11-26 ZA ZA859033A patent/ZA859033B/en unknown
- 1985-11-26 IL IL77152A patent/IL77152A0/en unknown
- 1985-11-26 FI FI854655A patent/FI854655A/en not_active Application Discontinuation
- 1985-11-26 NL NL8503257A patent/NL8503257A/en not_active Application Discontinuation
- 1985-11-26 JP JP60263974A patent/JPS61166395A/en active Pending
- 1985-11-26 FR FR8517437A patent/FR2573773A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
NL8503257A (en) | 1986-06-16 |
IT1191642B (en) | 1988-03-23 |
FR2573773A1 (en) | 1986-05-30 |
AU5034785A (en) | 1986-06-05 |
GB8529127D0 (en) | 1986-01-02 |
FI854655A (en) | 1986-05-27 |
DK547285D0 (en) | 1985-11-26 |
DE3541783A1 (en) | 1986-06-12 |
ZA859033B (en) | 1986-08-27 |
HU194309B (en) | 1988-01-28 |
GB2167760A (en) | 1986-06-04 |
SE8505568L (en) | 1986-05-27 |
PL256453A1 (en) | 1986-12-30 |
HUT39475A (en) | 1986-09-29 |
BE903695A (en) | 1986-05-22 |
DK547285A (en) | 1986-05-27 |
JPS61166395A (en) | 1986-07-28 |
FI854655A0 (en) | 1985-11-26 |
IL77152A0 (en) | 1986-04-29 |
IT8522982A0 (en) | 1985-11-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ES524816A0 (en) | PROCEDURE FOR THE PRODUCTION OF POLYPETHYDES THROUGH YEAST. | |
PL272698A1 (en) | Method for expressing human proapolyprotein a-j | |
ATE384798T1 (en) | POLYPEPTIDES WITH PHYTASE ACTIVITY AND NUCLEIC ACIDS ENCODING THEM | |
NO883592D0 (en) | ENZYME. | |
CN1070227A (en) | The DNA sequence of encoding gelonin polypeptide | |
KR910004803A (en) | The parenchymal cell growth factor of the liver, the gene encoding it, the method of producing the factor, and the transformant producing the factor | |
SE8101665L (en) | POLYPEPTIDFRAMSTELLNING | |
Negro et al. | Synthesis, purification, and characterization of human ciliary neuronotrophic factor from E. coli | |
SE8505568D0 (en) | EXPRESSION VECTORS AND METHOD FOR THEIR PRODUCTION | |
CA2197075A1 (en) | Method of Gene Therapy Using DNA Vectors Without Selection Marker Gene | |
EE03074B1 (en) | Recombinant eukaryotic cells, DNA sequences required for their production, method for constructing eukaryotic cells and method for production of secreted proteins and biomass | |
GB2229184B (en) | A gene coding for glucuronide permease | |
ES2111537T3 (en) | RECOMBINANT HUMAN PLACENTA RIBONUCLEASE INHIBITOR AND PRODUCTION METHOD. | |
AU2002365538A1 (en) | Production of eukaryotic proteins and nucleic acid molecules in c. elegans | |
DE69922071D1 (en) | Tumor suppressor gene Del-27 and the protein encoded thereby, process for their production and use | |
FR2450262A1 (en) | Plasmid(s) contg. DNA fragment attached to operon - for transformation into host bacterium on yeast to produce nutrient proteins, esp. ovalbumin from E. coli (NL 11.12.79) albumin | |
KR890010197A (en) | Expression vectors, transformed strains, and methods for producing the same, which are used to produce useful peptides from E. coli cells by genetic engineering methods | |
JPS6474988A (en) | Synthetic gene of cystatin c, corresponding recombinant plasmid and recombinant | |
IT1254316B (en) | Process for the preparation of acyl phosphatase using genetically modified cells and recombinant vectors and modified organisms which can be obtained using the process |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
NAV | Patent application has lapsed |
Ref document number: 8505568-9 Effective date: 19871030 |